Zika Virus
Dzina la malonda
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Kachilombo ka Zika ndi kagulu ka Flaviviridae, ndi kachilombo ka RNA komwe kamakhala ndi chingwe chimodzi chokhala ndi mainchesi 40-70nm. Ili ndi envelopu, ili ndi 10794 nucleotides, ndipo imayika 3419 amino acid. Malinga ndi genotype, imagawidwa mumtundu waku Africa komanso mtundu waku Asia. Matenda a Zika ndi matenda opatsirana odziletsa okha omwe amayamba chifukwa cha kachilombo ka Zika, kamene kamafala kwambiri polumidwa ndi udzudzu wa Aedes aegypti. Matendawa amakhala makamaka malungo, zidzolo, arthralgia kapena conjunctivitis, ndipo nthawi zambiri sapha. Malinga ndi World Health Organization, neonatal microcephaly ndi Guillain-Barre syndrome (Guillain-Barré syndrome) angagwirizane ndi matenda a Zika virus.
Channel
| FAM | Zika virus nucleic acid |
| Mtengo ROX | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | ≤30 ℃ & kutetezedwa ku kuwala |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | seramu yatsopano |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Makopi / ml |
| Mwatsatanetsatane | Gwiritsani ntchito zida kuti muwone zitsanzo za seramu zomwe zili ndi kachilombo ka Zika, ndipo zotsatira zake zimakhala zoipa. Zotsatira zoyeserera zosokoneza zikuwonetsa kuti kuchuluka kwa bilirubin mu seramu sikupitilira 168.2μmol/ml, kuchuluka kwa hemoglobin komwe kumapangidwa ndi hemolysis sikupitilira 130g/L, kuchuluka kwa lipid m'magazi sikuposa 65mmol/ml, kuchuluka kwa IgG mu seramu sikuposa 5mg/mL, kuzindikirika kwa virus kapena chikungue kulibe kachilomboka. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus ndi human genomic serum samples amasankhidwa kuti ayesedwe, ndipo zotsatira zake zikuwonetsa kuti palibe kusinthana pakati pa zida izi ndi tizilombo toyambitsa matenda tatchulazi. |
| Zida Zogwiritsira Ntchito | ABI 7500 Real-Time PCR SystemsABI 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kapena Purification Reagent (YDP315-R) ndi Tiangen Biotech(Beijing) Co.,Ltd.M'zigawoiyenera kuchotsedwa molingana ndi malangizo a m'zigawo, ndipo voliyumu yotulutsa yovomerezeka ndi 140 μL ndipo voliyumu yovomerezeka ndi 60 μL.
Njira 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). M'zigawozo ziyenera kuchotsedwa malinga ndi malangizo. Voliyumu yotulutsa ndi 200 μL, ndipo voliyumu yovomerezeka ndi 80μL.
