Kachilombo ka Zika
Dzina la chinthu
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Kachilombo ka Zika ndi ka mtundu wa Flaviviridae, ndi kachilombo ka RNA kokhala ndi chingwe chimodzi chokhala ndi mainchesi a 40-70nm. Kali ndi chivundikiro, kali ndi ma nucleotide 10794, ndipo kamalemba ma amino acid 3419. Malinga ndi mtundu wa majini, kamagawidwa m'magulu a Africa ndi Asia. Matenda a Zika ndi matenda opatsirana omwe amadziletsa okha omwe amayamba chifukwa cha kachilombo ka Zika, komwe kamafalikira makamaka kudzera mu kulumidwa ndi udzudzu wa Aedes aegypti. Zizindikiro zake zachipatala makamaka ndi malungo, ziphuphu, arthralgia kapena conjunctivitis, ndipo nthawi zambiri siipha. Malinga ndi World Health Organization, ma neonatal microcephaly ndi Guillain-Barre syndrome (Guillain-Barré syndrome) zitha kugwirizanitsidwa ndi kachilombo ka Zika.
Njira
| FAM | Asidi ya nucleic ya kachilombo ka Zika |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤30℃ & yotetezedwa ku kuwala |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | seramu watsopano |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Makopi 500/mL |
| Kufotokozera Mwapadera | Gwiritsani ntchito chida ichi kuti mupeze zitsanzo za seramu zomwe zili ndi kachilombo ka Zika, ndipo zotsatira zake zimakhala zoipa. Zotsatira za mayeso oletsa zimasonyeza kuti pamene kuchuluka kwa bilirubin mu seramu sikupitirira 168.2μmol/ml, kuchuluka kwa hemoglobin komwe kumapangidwa ndi hemolysis sikupitirira 130g/L, kuchuluka kwa mafuta m'magazi sikupitirira 65mmol/ml, kuchuluka konse kwa IgG mu seramu sikupitirira 5mg/mL, palibe chomwe chimakhudza kachilombo ka dengue, kachilombo ka Zika kapena chikungunya. Kachilombo ka Hepatitis A, kachilombo ka Hepatitis B, kachilombo ka Hepatitis C, kachilombo ka Herpes, kachilombo ka Eastern equine encephalitis, Hantavirus, kachilombo ka Bunya, kachilombo ka West Nile ndi zitsanzo za seramu ya genomic ya anthu zimasankhidwa kuti ziyesedwe mosiyanasiyana, ndipo zotsatira zake zikusonyeza kuti palibe kusinthana pakati pa chida ichi ndi tizilombo toyambitsa matenda tatchulidwa pamwambapa. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a ABI 7500 Real-TimeMachitidwe a PCR a ABI 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Kiti Yaing'ono ya QIAamp Viral RNA (52904), Nucleic Acid Extraction kapena Purification Reagent (YDP315-R) ndi Tiangen Biotech(Beijing) Co.,Ltd.Kuchotsaayenera kuchotsedwa motsatira malangizo ochotsera, ndipo kuchuluka koyenera kochotsera ndi 140 μL ndipo kuchuluka koyenera kochotsera ndi 60 μL.
Njira yachiwiri.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Chochotsacho chiyenera kuchotsedwa motsatira malangizo. Voliyumu ya chitsanzo chochotsera ndi 200 μL, ndipo voliyumu yovomerezeka ya elution ndi 80μL.
