Staphylococcus Aureus ndi Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira bwino za staphylococcus aureus ndi methicillin-resistant staphylococcus aureus nucleic acid mu zitsanzo za sputum, zitsanzo za m'mphuno ndi pakhungu ndi minofu yofewa mu vitro.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-OT062 Staphylococcus Aureus ndi Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)

Satifiketi

CE

Epidemiology

Staphylococcus aureus ndi amodzi mwa mabakiteriya ofunikira amtundu wa nosocomial.Staphylococcus aureus (SA) ndi ya staphylococcus ndipo imayimira mabakiteriya a Gram-positive, omwe amatha kupanga mitundu yambiri ya poizoni ndi ma enzymes owononga.Mabakiteriya ali ndi mawonekedwe a kugawa kwakukulu, mphamvu yamphamvu ya pathogenicity komanso kukana kwakukulu.Thermostable nuclease jini (nuc) ndi jini yotetezedwa kwambiri ya staphylococcus aureus.

Channel

FAM jini ya mecA yosamva methicillin
Mtengo ROX

Ulamuliro Wamkati

CY5 staphylococcus aureus nuc gene

Magawo aukadaulo

Kusungirako ≤-18 ℃ & kutetezedwa ku kuwala
Alumali moyo 12 miyezi
Mtundu wa Chitsanzo sputum, zitsanzo za matenda a pakhungu ndi minofu yofewa, ndi zitsanzo za swab za m'mphuno
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL mabakiteriya osamva methicillin.Chidacho chikazindikira mtundu wa LoD, 1000/mL staphylococcus aureus imatha kudziwika.
Mwatsatanetsatane Kafukufuku woyandikana nawonso akuwonetsa kuti zida izi sizikhala ndi matenda ena a methyolocckus, acticilialla-stionolocae, klebillalla chibayo ife mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Zida Zogwiritsira Ntchito Applied Biosystems 7500 Real-Time PCR Systems

Zithunzi za QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR dongosolo

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Kuyenda Ntchito

Njira 1.

Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. itha kugwiritsidwa ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Onjezani 200µL ya saline wamba pamadzi okonzedwa, ndipo masitepe otsatirawa atengedwe molingana ndi malangizo, ndipo voliyumu yoyeserera ndi 80µL.

Njira 2.

Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 1mL ya saline wamba pamadzi mutatha kutsuka ndi saline wamba, kenaka sakanizani bwino.Centrifuge pa 13,000r/mphindi kwa mphindi 5, chotsani supernatant (sungani 10-20µL ya supernatant), ndipo tsatirani malangizo a m'zigawo zotsatira.

Regent yovomerezeka yochotsa: Nucleic Acid Extraction kapena Purification Reagent (YDP302) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd.Kuchotsa kuyenera kuchitidwa mosamalitsa molingana ndi gawo 2 la buku la malangizo.Ndibwino kuti mugwiritse ntchito madzi a RNase ndi DNase kuti mutengeko ndi mphamvu ya 100µL.

 


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