Adenovirus Universal
Dzina la malonda
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Human adenovirus (HAdV) ndi ya mtundu wa Mammalian adenovirus, yomwe ndi kachilombo ka DNA kozungulira kawiri kopanda envelopu.Adenoviruses omwe apezeka mpaka pano akuphatikizapo 7 subgroups (AG) ndi mitundu 67, yomwe 55 serotypes ndi tizilombo toyambitsa matenda kwa anthu.Mwa iwo, omwe angayambitse matenda am'mapapo am'mimba ndi gulu B (Mtundu 3, 7, 11, 14, 16, 21, 50, 55), Gulu C (Mtundu 1, 2, 5, 6, 57) ndi Gulu E. (Mtundu wa 4), ndipo ukhoza kutsogolera ku matenda otsekula m'mimba ndi Gulu F (Mtundu 40 ndi 41)[1-8].Mitundu yosiyanasiyana imakhala ndi zizindikiro zosiyanasiyana zachipatala, koma makamaka matenda a kupuma.Matenda opumira omwe amayamba chifukwa cha matenda opumira m'thupi la munthu amakhala 5% ~ 15% ya matenda opuma padziko lonse lapansi, ndi 5% -7% ya matenda apadziko lonse lapansi okhudza kupuma kwaubwana[9].Adenovirus imapezeka m'madera osiyanasiyana ndipo imatha kutenga kachilomboka chaka chonse, makamaka m'madera odzaza anthu, omwe nthawi zambiri amakhala ndi miliri, makamaka m'masukulu ndi m'misasa ya asilikali.
Channel
| FAM | adenovirus padziko lonsenucleic acid |
| Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Nasopharyngeal swab,Pakhosi pakhosi |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Makopi / ml |
| Mwatsatanetsatane | a) Yesani mbiri yoyipa yamakampani omwe ali ndi zida, ndipo zotsatira zake zimakwaniritsa zofunikira. b) Gwiritsani ntchito zidazi kuti muzindikire ndipo palibe kuyanjananso ndi tizilombo toyambitsa matenda (monga kachilombo ka Influenza A, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) kapena mabakiteriya (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, etc.). |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhouukadaulo wa bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems |
Kuyenda Ntchito
(1) Analimbikitsa m'zigawo reagent:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Kuchotsa kuyenera kuchitidwa motsatira malangizo.Chitsanzo chotengedwa ndi odwala'nasopharyngeal swab kapena pakhosi swab zitsanzo zosonkhanitsidwa pamalopo.Onjezani zitsanzo muzotulutsa zotulutsa za Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kusakaniza bwino, ikani kutentha kwa mphindi 5, chotsani ndikutembenuza ndikusakaniza bwino kuti mupeze DNA ya chitsanzo chilichonse.
(2) Analimbikitsa m'zigawo reagent:Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).opaleshoni ayenera kuchitidwa mosamalitsa malinga ndi malangizo.Voliyumu yotulutsidwa ndi 200μL, ndianalimbikitsa elution voliyumuis80μl pa.
(3) Analimbikitsa m'zigawo reagent: Nucleic Acid m'zigawo kapena Purification Reagent (YDP315ndi Tiangen Biotech (Beijing) Co., Ltd., ndintchito ayenera kuchitidwa mosamalitsa malinga ndi malangizo.Voliyumu yotulutsidwa ndi 200μL, ndianalimbikitsa elution voliyumuis80μl pa.
