Adenovirus Universal
Dzina la chinthu
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Adenovirus ya anthu (HAdV) ili m'gulu la adenovirus ya Mammalian, yomwe ndi kachilombo ka DNA kokhala ndi mizere iwiri popanda kufalikira. Ma Adenovirus omwe apezeka mpaka pano akuphatikizapo magulu ang'onoang'ono 7 (AG) ndi mitundu 67, yomwe ma serotype 55 ndi omwe amachititsa anthu kudwala. Pakati pawo, matenda opatsirana m'mapapo ndi gulu B (Mitundu 3, 7, 11, 14, 16, 21, 50, 55), Gulu C (Mitundu 1, 2, 5, 6, 57) ndi Gulu E (Mtundu 4), ndipo matenda opatsirana m'mimba ndi Gulu F (Mitundu 40 ndi 41) [1-8]. Mitundu yosiyanasiyana ili ndi zizindikiro zosiyanasiyana zachipatala, koma makamaka matenda opatsirana m'mapapo. Matenda opuma omwe amayamba chifukwa cha matenda opatsirana m'mapapo m'thupi la munthu ndi 5% ~ 15% ya matenda opuma padziko lonse lapansi, ndi 5% - 7% ya matenda opuma padziko lonse lapansi [9]. Kachilombo ka Adenovirus kamapezeka m'malo osiyanasiyana ndipo kamatha kufalikira chaka chonse, makamaka m'malo odzaza anthu, omwe nthawi zambiri amafalikira m'deralo, makamaka m'masukulu ndi m'misasa ya asilikali.
Njira
| FAM | adenovirus yapadziko lonseasidi wa mu nyukiliyasi |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Nsalu ya m'mphuno，Chitsulo cha pakhosi |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makope 300/mL |
| Kufotokozera Mwapadera | a) Yesani zizindikiro zovomerezeka za kampani pogwiritsa ntchito zida, ndipo zotsatira za mayeso zikukwaniritsa zofunikira. b) Gwiritsani ntchito chida ichi kuti mupeze ndipo palibe njira yolumikizirana ndi tizilombo tina toyambitsa matenda opuma (monga kachilombo ka Influenza A, kachilombo ka Influenza B, kachilombo ka Respiratory syncytial, kachilombo ka Parainfluenza, Rhinovirus, Human metapneumovirus, ndi zina zotero) kapena mabakiteriya (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, ndi zina zotero). |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCRs (FQD-96AHangzhouUkadaulo wa Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Machitidwe a BioRad CFX96 Real-Time PCR, Machitidwe a BioRad CFX Opus 96 Real-Time PCR |
Kuyenda kwa Ntchito
(1) Chotsukira chomwe chimalimbikitsidwa:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kuchotsa kuyenera kuchitika motsatira malangizo. Chitsanzo chochotsedwacho ndi odwala.'Zitsanzo za swab ya nasopharyngeal kapena swab ya pakhosi zomwe zasonkhanitsidwa pamalopo. Onjezani zitsanzozo mu reagent yotulutsa chitsanzo ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kuti zisakanizike bwino, ziyikeni kutentha kwa chipinda kwa mphindi 5, tulutsani kenako mutembenuzire ndikusakaniza bwino kuti mupeze DNA ya chitsanzo chilichonse.
(2) Chotsukira chomwe chimalimbikitsidwa:Macro & Micro-Test Zachilombo Kiti ya DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ntchitoyi iyenera kuchitika motsatira malangizo. Kuchuluka kwa chitsanzo chomwe chatengedwa ndi 200μLndivoliyumu yovomerezeka ya elutionis80μL.
(3) Reagent yovomerezeka yochotsera: Nucleic Acid Extraction kapena Purification Reagent (YDP)315) ndi Tiangen Biotech (Beijing) Co.,Ltd., ndiNtchitoyi iyenera kuchitika motsatira malangizo. Kuchuluka kwa chitsanzo chomwe chatulutsidwa ndi 200μLndivoliyumu yovomerezeka ya elutionis80μL.
