Mitundu 4 ya Ma virus Opumira Nucleic Acid

Kufotokozera Kwachidule:

Zidazi zimagwiritsidwa ntchito pozindikira za SARS-CoV-2, kachilombo ka fuluwenza A, kachilombo ka fuluwenza B ndi kupuma kwa syncytial virus nucleic acid mu zitsanzo za swab za oropharyngeal.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT075-Fluorescence PCR yowumitsidwa ya HWTS-RT075-Fluorescence PCR

Epidemiology

Matenda a Corona Virus 2019, omwe amatchedwa "COVID-19", amatanthauza chibayo chomwe chimayambitsidwa ndi matenda a SARS-CoV-2.SARS-CoV-2 ndi coronavirus yamtundu wa β.COVID-19 ndi matenda opatsirana kwambiri opatsirana, ndipo anthu nthawi zambiri amatengeka.Pakadali pano, gwero la matendawa ndi odwala omwe ali ndi kachilombo ka SARS-CoV-2, ndipo anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero la matenda.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1-14, makamaka masiku 3-7.Kutentha thupi, chifuwa chowuma ndi kutopa ndizo zizindikiro zazikulu.Odwala ochepa anali ndi zizindikiro monga kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsegula m'mimba, ndi zina zotero.

Influenza, yomwe imadziwika kuti "flu", ndi matenda opatsirana omwe amayamba chifukwa cha kachilombo ka fuluwenza.Ndi opatsirana kwambiri.Amafala makamaka ndi kutsokomola ndi kuyetsemula.Nthawi zambiri imayamba masika ndi nyengo yozizira.Mavairasi a chimfine amagawidwa mu fuluwenza A (IFV A), fuluwenza B (IFV B), ndi Influenza C (IFV C) mitundu itatu, onse a ma virus omata, amayambitsa matenda a anthu makamaka kwa ma virus a fuluwenza A ndi B, ndi amodzi. - kachilombo ka RNA kamene kamakhala kozungulira.Chimfine A ndi matenda pachimake kupuma, kuphatikizapo H1N1, H3N2 ndi subtypes ena, amene amakonda masinthidwe ndi kufalikira padziko lonse."Shift" amatanthauza kusintha kwa kachilombo ka fuluwenza A, zomwe zimapangitsa kuti kachilombo katsopano ka "subtype".Ma virus a fuluwenza B amagawidwa m'mizere iwiri, Yamagata ndi Victoria.Kachilombo ka fuluwenza B kamakhala ndi antigenic drift, ndipo imazemba kuyang'anira chitetezo cha mthupi cha munthu ndikuchichotsa kudzera mukusintha kwake.Komabe, liwiro la chimfine cha B ndilocheperako kuposa kachilombo ka fuluwenza A.Kachilombo ka fuluwenza B kamayambitsanso matenda a kupuma kwa anthu ndikuyambitsa miliri.

Respiratory syncytial virus (RSV) ndi kachilombo ka RNA, komwe kamachokera ku banja la paramyxoviridae.Amafalitsidwa ndi madontho a mpweya ndi kukhudzana kwambiri ndipo ndiye tizilombo toyambitsa matenda a m'munsi mwa kupuma kwa makanda.Makanda omwe ali ndi kachilombo ka RSV amatha kukhala ndi bronchiolitis ndi chibayo, zomwe zimakhudzana ndi mphumu mwa ana.Makanda kwambiri zizindikiro, kuphatikizapo kutentha thupi, rhinitis, pharyngitis ndi laryngitis, ndiyeno bronchiolitis ndi chibayo.Ana ochepa odwala angakhale ovuta ndi otitis TV, pleurisy ndi myocarditis, etc. Chapamwamba kupuma thirakiti matenda ndi chizindikiro chachikulu cha matenda akuluakulu ndi ana okulirapo.

Channel

FAM SARS-CoV-2
VIC (HEX) RSV
CY5 IFV A
Mtengo ROX IFV B
NED

Ulamuliro Wamkati

Magawo aukadaulo

Kusungirako 2-8 ° C
Alumali moyo 12 miyezi
Mtundu wa Chitsanzo Oropharyngeal swab
Ct ≤38
LoD SARS-CoV-2: 150Copies/mL

Influenza A virus/Influenza B virus/Respiratory syncytial virus: 300Copies/mL

Mwatsatanetsatane Palibe cross-reactivity with human virus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, kachilombo ka m'mapapo a anthu, kachilombo ka epstein-barr, kachilombo ka chikuku, kachilombo ka cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium TB, utsi aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ndi cryptococcus wobadwa kumene ndi munthu genomic nucleic acid.
Zida Zogwiritsira Ntchito Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems

Kuyenda Ntchito

Njira 1.

Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Njira 2.

Zomwe zimalangizidwa m'zigawo: Nucleic Acid Extraction kapena Purification Reagent(YDP302) yolembedwa ndi Tiangen Biotech(Beijing) Co.,Ltd.


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