Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira bwino kwa MERS coronavirus nucleic acid mu nasopharyngeal swabs ndi Middle East Respiratory Syndrome (MERS) coronavirus.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

Middle East Respiratory Syndrome coronavirus (MERS-CoV), β-coronavirus yomwe imayambitsaMatenda opumira mwa anthu, adadziwika koyamba mwa wodwala wakufa wazaka 60 waku Saudi Arabia pa 24th July, 2012. Kuwonetsera kwachipatala kwa matenda a MERS-CoV kumachokera ku chikhalidwe cha asymptomatic kapena zizindikiro za kupuma pang'ono mpaka matenda aakulu opuma kupuma ngakhale imfa.

Channel

FAM MERS kachilombo ka RNA
VIC (HEX)

Ulamuliro Wamkati

Magawo aukadaulo

Kusungirako

≤-18 ℃ Mumdima

Alumali moyo

9 miyezi

Mtundu wa Chitsanzo

Mwatsopano anasonkhanitsidwa nasopharyngeal swabs

CV

≤5.0%

Ct

≤38

LoD

1000 Makopi / ml

Mwatsatanetsatane

Palibe cholumikizirana ndi ma coronaviruses aumunthu a SARSr-CoV ndi ma virus ena omwe wamba.

Zida Zomwe Zingagwiritsidwe Ntchito:

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Kuyenda Ntchito

Njira 1.

Zopangira zopangira zovomerezeka: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd.

Njira 2.

Zopangira zopangira zovomerezeka: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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