Munthu CYP2C19 Gene Polymorphism

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative kuzindikira kwa polymorphism ya CYP2C19 jini CYP2C19*2 (rs4244285, c.681G>A), CYP2C19*3 (rs4986893, c.636G>A), CYP2C19*2685 (rs4810, c. > T) mu genomic DNA yamagazi athunthu amunthu.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)

Satifiketi

CE/TFDA

Epidemiology

CYP2C19 ndi imodzi mwama enzymes ofunikira omwe amasokoneza ma enzymes m'banja la CYP450.Magawo ambiri amkati komanso pafupifupi 2% yamankhwala amapangidwa ndi CYP2C19, monga kagayidwe ka antiplatelet aggregation inhibitors (monga clopidogrel), proton pump inhibitors (omeprazole), anticonvulsants, ndi zina zotero. mankhwala okhudzana.Kusintha kwa mfundo izi za *2 (rs4244285) ndi *3 (rs4986893) kumayambitsa kutayika kwa ntchito ya enzyme yosungidwa ndi jini ya CYP2C19 komanso kufooka kwa gawo la gawo lapansi la metabolic, ndikuwonjezera kuchuluka kwa magazi, kuti ayambitse zovuta zoyipa zokhudzana ndi ndende ya magazi.* 17 (rs12248560) imatha kukulitsa ntchito ya enzyme yosungidwa ndi jini ya CYP2C19, kupanga ma metabolites yogwira, komanso kukulitsa kuletsa kuphatikizika kwa mapulateleti ndikuwonjezera chiwopsezo chamagazi.Kwa anthu omwe ali ndi kagayidwe kake ka mankhwala osokoneza bongo, kumwa Mlingo wabwinobwino kwa nthawi yayitali kumayambitsa poizoni komanso zotsatirapo zoyipa: makamaka kuwonongeka kwa chiwindi, kuwonongeka kwa dongosolo la hematopoietic, kuwonongeka kwa dongosolo lamanjenje, ndi zina zambiri, zomwe zingayambitse imfa kwambiri.Malinga ndi kusiyanasiyana kwa kagayidwe kake ka mankhwala, nthawi zambiri amagawidwa m'magulu anayi, omwe ndi ultra-fast metabolism (UM, *17/*17,*1/*17), kagayidwe kachakudya (RM,*1/*1) ), metabolism yapakatikati (IM, *1/*2, *1/*3), slow metabolism (PM, *2/*2, *2/*3, *3/*3).

Channel

FAM CYP2C19*2
CY5 CYP2C9*3
Mtengo ROX CYP2C19*17
VIC/HEX IC

Magawo aukadaulo

Kusungirako Madzi: ≤-18 ℃
Alumali moyo 12 miyezi
Mtundu wa Chitsanzo Mwazi watsopano wa EDTA anticoagulated
CV ≤5.0%
LoD 1.0ng/μL
Mwatsatanetsatane Palibe kuyanjananso komwe kumayenderana kwambiri (CYP2C9 jini) mu genome yamunthu.Kusintha kwa CYP2C19 * 23, CYP2C19 * 24 ndi CYP2C19 * 25 malo omwe ali kunja kwa mndandanda wa zidazi alibe mphamvu pakuwona kwa chida ichi.
Zida Zogwiritsira Ntchito Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Kuyenda Ntchito

Regent yovomerezeka yochotsa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The m'zigawo ayenera yotengedwa malinga ndi malangizo.Voliyumu yotulutsa ndi 200μL, ndipo voliyumu yovomerezeka ndi 100μL.

Zopangira zopangira zovomerezeka: Wizard® Genomic DNA Purification Kit (Catalog No.: A1120) by Promega, Nucleic Acid Extraction or Purification Reagent(YDP348) yolembedwa ndi Tiangen Biotech(Beijing) Co.,Ltd.ziyenera kuchotsedwa molingana ndi malangizo a m'zigawo, ndipo voliyumu yotulutsa yovomerezeka ndi 200 μL ndipo voliyumu yovomerezeka ndi 160 μL.


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