Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Isoniazid Resistance(INH)

Kufotokozera Kwachidule:

Izi ndizoyenera kuzindikira kwamtundu wa Mycobacterium tuberculosis DNA mu zitsanzo za sputum za anthu mu vitro, komanso kusintha kwa homozygous mu 507-533 amino acid codon region (81bp, rifampicin resistance determining region) ya jini ya rpoB yomwe imayambitsa chifuwa chachikulu cha Mycobacterium. rifampicin kukana.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, yomwe imadziwika kuti Tubercle bacillus (TB), ndi tizilombo toyambitsa matenda timene timayambitsa chifuwa chachikulu.Pakali pano, mankhwala omwe amagwiritsidwa ntchito kwambiri pamzere woyamba wa chifuwa chachikulu ndi isoniazid, rifampicin ndi ethambutol, ndi zina zotero. Mankhwala amtundu wachiwiri oletsa chifuwa chachikulu ndi fluoroquinolones, amikacin ndi kanamycin, ndi zina zotero. Mankhwala atsopano opangidwa ndi linezolid, bedaquiline ndi delamani, ndi zina zotero. Komabe, chifukwa chogwiritsa ntchito molakwika mankhwala odana ndi chifuwa chachikulu komanso mawonekedwe a cell khoma la mycobacterium TB, mycobacterium TB imakulitsa kukana kwa mankhwala ku anti-TB, zomwe zimabweretsa zovuta zazikulu pakupewa ndi kuchiza chifuwa chachikulu.

Channel

Dzina Lachindunji			Mtolankhani	Quencher
Reaction BufferA	Reaction BufferB	Reaction BufferC	Mtolankhani	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Palibe
rpoB 520-527	rpoB 527-533	/	CY5	Palibe
/	/	Ulamuliro wamkati	HEX(VIC)	Palibe

Reaction BufferD	Mtolankhani	Quencher
Chigawo cholimbikitsa cha InhA -15C>T, -8T>A, -8T>C	FAM	Palibe
KatG 315 kodi 315G>A,315G>C	CY5	Palibe
Chigawo cholimbikitsa cha AhpC -12C>T, -6G>A	Mtengo ROX	Palibe

Magawo aukadaulo

Kusungirako	≤-18 ℃
Alumali moyo	12 miyezi
Mtundu wa Chitsanzo	makoko
CV	≤5.0%
LoD	LoD ya mycobacterium tuberculosis national reference is 50 bacteria/mL.LoD ya mtundu wakuthengo wolimbana ndi rifampicin ndi 2×10³mabakiteriya/mL, ndipo LoD ya mtundu wosinthika ndi 2×10³mabakiteriya/mL.LoD ya mabakiteriya olimbana ndi isoniazid akutchire ndi 2x10³bacteria/mL, ndipo The LoD ya mabakiteriya osasintha ndi 2x10³mabakiteriya/mL.
Mwatsatanetsatane	Zotsatira zoyesa pamtanda zidawonetsa kuti panalibe njira yolumikizirana pozindikira matupi athu, mabakiteriya ena omwe si a TB ndi tizilombo toyambitsa matenda a chibayo ndi zida izi;Panalibe kusintha komwe kunapezeka pamalo osinthika amitundu ina yosamva mankhwala mumtundu wakuthengo wa Mycobacterium tuberculosis.
Zida Zogwiritsira Ntchito	SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Real-Time PCR System, Tekinoloje ya Hangzhou Bioer QuantGene 9600 Real-Time PCR System, Zithunzi za QuantStudio^®5 Real-Time PCR System.