Staphylococcus Aureus ndi Staphylococcus Aureus Nucleic Acid Detection Kit Yosagonjetsedwa ndi Methicillin
Dzina la chinthu
Staphylococcus Aureus ndi Staphylococcus Aureus Yokana Methicillin Nucleic Acid Detection Kit (Fluorescence PCR)
Kugwiritsa Ntchito Koyenera
Chida ichi chimagwiritsidwa ntchito pozindikira bwino staphylococcus aureus ndi staphylococcus aureus nucleic acids yolimbana ndi methicillin m'masampulu a makoswe a anthu, zitsanzo za swab ya m'mphuno ndi zitsanzo za matenda a pakhungu ndi minofu yofewa mu vitro. Kuyesa zitsanzo za sputum ndi swab ya m'mphuno kumagwiritsidwa ntchito m'mabungwe azachipatala kuti apewe ndikuwongolera matenda a MRSA nosocomial mwa odwala omwe ali m'chipatala, kuphatikiza odwala odwala kwambiri, odwala opaleshoni ndi odwala osamalira nthawi yayitali; Ingagwiritsidwenso ntchito pozindikira odwala omwe akukayikiridwa kuti ndi Staphylococcus Aureus ndi Methicillin-Resistant Staphylococcus Aureus; Kapena mogwirizana ndi mayeso ena a labotale monga ma microorganisms kuti athandize kuzindikira matenda a pakhungu ndi minofu yofewa ya MRSA/SA. Zotsatira za mayeso sizigwiritsidwa ntchito mwachindunji kutsogolera kugwiritsa ntchito maantibayotiki.
Epidemiology
Staphylococcus aureus ndi imodzi mwa mabakiteriya ofunikira kwambiri omwe amayambitsa matenda a nosocomial. Staphylococcus aureus (SA) ndi ya staphylococcus ndipo imayimira mabakiteriya a Gram-positive, omwe amatha kupanga poizoni wosiyanasiyana ndi ma enzyme olowa. Mabakiteriyawa ali ndi mawonekedwe ofalikira kwambiri, mphamvu yamphamvu yopatsira matenda komanso kukana kwambiri. Jini la nuclease lotenthetsera (nuc) ndi jini losungidwa bwino la staphylococcus aureus.
M'zaka zaposachedwa, chifukwa cha kugwiritsa ntchito kwambiri mahomoni ndi mankhwala ophera chitetezo chamthupi komanso kugwiritsa ntchito molakwika maantibayotiki a broad-spectrum, matenda a nosocomial omwe amayamba chifukwa cha Methicillin-resistant Staphylococcus aureus (MRSA) mu Staphylococcus akhala akukwera. Chiwerengero chapakati cha MRSA chomwe chimapezeka mdziko lonse chinali 30.2% mu 2019 ku China. MRSA imagawidwa m'magulu awiri: MRSA (HA-MRSA), MRSA (CA-MRSA) ndi MRSA (LA-MRSA). CA-MRSA, HA-MRSA, LA-MRSA ali ndi kusiyana kwakukulu mu microbiology, kukana kwa mabakiteriya (monga, HA-MRSA imasonyeza kukana mankhwala ambiri kuposa CA-MRSA) ndi makhalidwe azachipatala (monga malo omwe matenda amafalikira). Malinga ndi makhalidwe amenewa, CA-MRSA ndi HA-MRSA zitha kusiyanitsidwa. Komabe, kusiyana pakati pa CA-MRSA ndi HA-MRSA kukuchepa chifukwa cha kuyenda kosalekeza kwa anthu pakati pa zipatala ndi madera. MRSA imakana mankhwala ambiri, sikuti imakana mankhwala opha tizilombo toyambitsa matenda a β-lactam okha, komanso imakana aminoglycosides, macrolides, tetracyclines ndi quinolones mpaka madigiri osiyanasiyana. Pali kusiyana kwakukulu m'madera osiyanasiyana pa kuchuluka kwa mankhwala opha tizilombo komanso njira zosiyanasiyana. MRSA imatha kulowa m'thupi kudzera m'mabala a pakhungu, m'mabowo a tsitsi, m'magazi ndi zina zomwe zimayambitsa matenda opatsirana, ndipo odwala matenda a pakhungu ndi kupsa amakhala pachiwopsezo chachikulu chotenga matenda a MRSA. Chibayo ndi chimodzi mwa matenda ofala kwambiri a MRSA, ndipo chithandizo chosayenera ndi chifukwa chachikulu cha imfa zambiri.
Magawo aukadaulo
| Malo Osungirako | ≤ -18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Makodzo, swab ya m'mphuno, chitsanzo cha matenda a khungu ndi minofu yofewa, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Kufotokozera Mwapadera | Mayeso a cross-reactivity akuwonetsa kuti kit iyi ilibe cross reactivity ndi matenda ena opatsirana kupuma monga staphylococcus aureus yomwe imakhudzidwa ndi methicillin, staphylococcus yomwe imatsutsana ndi methicillin, staphylococcus yomwe imakana methicillin, staphylococcus epidermidis yomwe imakana methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zida Zogwiritsidwa Ntchito | Mtundu Woyamba: Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Mtundu Wachiwiri: HWTS-EudemonTM AIO800 |
Kuyenda kwa Ntchito
Ma reagent ndi zida zimafunika koma siziperekedwa
Mtundu Woyamba
(1) Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Chitsanzo cha kusungunula: 4% NaOH kapena Sputasol kuchokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Mchere wamba.
Mtundu Wachiwiri:
Kit ya Macro & Micro-Test General DNA/RNA (HWTS-3019-8) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Zinthu zogwiritsidwa ntchito zimafunika koma siziperekedwa:Malangizo opanda RNase/DNase, Magolovesi otayidwa, machubu a EP opanda RNase/DNase, mipiringidzo ya machubu 8 a PCR.
