Staphylococcus Aureus ndi Methicillin-Petant-Staphylococcus Aureus (Mrsta / SA)
Dzina lazogulitsa
HWTS-OT062 Staphylococcus Aureus ndi methicillin-osagwirizana ndi Staphylococcus Aureus (Mrsta / SA) Nucleic Acid Kit (Fluorescence PCR)
Chiphaso
CE
Epidemiology
Staphylococcus Aureus ndi amodzi mwa tizilombo toyambitsa matenda a nosocomial. Staphylococcus Aureus (SA) ndi ya staphylocckus ndipo ndi woimira mabakiteriya oyenera, omwe amatha kupanga zoopsa ndi ma enzyme obwera. Mabakiteriya ali ndi mawonekedwe omwe amagawidwa kwakukulu, pathanthwe olimba komanso kukana kwakukulu. Nyuzi ya Mormostaser Eaclearse (NUC) ndi mtundu wofunikira kwambiri wa Stapphyloccus Aureus.
Ngalande
| Banja | MethIcillin-pokana Meca Gene |
| Rox | Kuwongolera kwamkati |
| Cy5 | Staphylococcus Aureus Nuc Gene |
Magawo aluso
| Kusunga | ≤-18 ℃ & Otetezedwa ku kuwala |
| Alumali-Moyo | Miyezi 12 |
| Mtundu wofanana | Sputum, khungu ndi minofu yamagetsi yofewa, ndi ma swab swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lodu | 1000 Cfu / ml Staphylococcus Aureus, 1000 Cfu / ml methicillin-bacterial-kapena mabakiteriya osagwirizana. Pamene Kit In Azindikire National Lod, 1000 / ml Staphylococcus Aureus ikhoza kupezeka |
| Chifanizo | Kafukufuku woyandikana nawonso akuwonetsa kuti zida izi sizikhala ndi matenda ena opumira monga methicillin Baumannii, propus mirabilis, elobacter zovala, streptococcus chibayo, a Altida a Albilos Albilafila, a Leaxella paramtalis, neissella meningitidis, hamophilus ferudezae. |
| Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Makupala®5 pcr yell pcr Slan-96P yeniyeni PCR Systems Kuwala®480 pcr pcr system Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Njira 1.
Macro & Bigro-Sport DNA / RNA Kit (HWTS-3019) ndi Jiangsu-Mecro-Mecro-Medicle Medic Acid Exctor (HWTS-3006C, 3006b). Onjezani miliri ya 200μl yambiri yotsogola, ndipo njira zotsatirazi ziyenera kutulutsidwa molingana ndi malangizo, ndipo kuchuluka kwa kufalikira ndi 80μl.
Njira 2.
Macro & Mayeso oyeserera a Macro-Micro-Micro-3005-8) ndi Jiangsu Macro & Tean-Medic Med-Tech CO., DTD., sakanizani bwino mchere. Centerrifuge ku 13,000r / min kwa mphindi 5, chotsani sulnatant ya supernant ya supernat ya supernanant), ndikutsatira malangizowo.
Cholinga cholimbikitsidwa: Ecleic Acid Exprection kapena kuyeretsa (ydp302) ndi tiangen biotech (Beijing), LTD. Kuchotsa kuyenera kuchitika molingana ndi gawo 2 la buku la Mabuku. Ndikulimbikitsidwa kugwiritsa ntchito madzi ndi dnase - madzi opanda ma dnase a erlution ndi kuchuluka kwa 100μC.
