Staphylococcus Aureus ndi Staphylococcus Aureus Yosagonjetsedwa ndi Methicillin (MRSA/SA)
Dzina la chinthu
HWTS-OT062 Staphylococcus Aureus ndi Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Staphylococcus aureus ndi imodzi mwa mabakiteriya ofunikira kwambiri omwe amayambitsa matenda a nosocomial. Staphylococcus aureus (SA) ndi ya staphylococcus ndipo imayimira mabakiteriya a Gram-positive, omwe amatha kupanga poizoni wosiyanasiyana ndi ma enzyme olowa. Mabakiteriyawa ali ndi mawonekedwe ofalikira kwambiri, mphamvu yamphamvu yopatsira matenda komanso kukana kwambiri. Jini la nuclease lotenthetsera (nuc) ndi jini losungidwa bwino la staphylococcus aureus.
Njira
| FAM | jini la mecA losagonjetsedwa ndi methicillin |
| ROX | Kulamulira Kwamkati |
| CY5 | staphylococcus aureus nuc gene |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ & yotetezedwa ku kuwala |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | zitsanzo za makoswe, matenda a khungu ndi minofu yofewa, ndi zitsanzo za swab ya m'mphuno |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL mabakiteriya osagonja ku methicillin. Pamene chipangizocho chizindikira chizindikiro cha dziko lonse cha LoD, 1000/mL staphylococcus aureus imatha kupezeka. |
| Kufotokozera Mwapadera | Mayeso a cross-reactivity akuwonetsa kuti kit iyi ilibe cross reactivity ndi matenda ena opatsirana kupuma monga staphylococcus aureus yomwe imakhudzidwa ndi methicillin, staphylococcus yomwe imatsutsana ndi methicillin, staphylococcus yomwe imakana methicillin, staphylococcus epidermidis yomwe imakana methicillin, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Onjezani 200µL ya saline wamba ku precipitate yokonzedwa, ndipo njira zotsatirazi ziyenera kuchotsedwa motsatira malangizo, ndipo voliyumu yovomerezeka ya elution ndi 80µL.
Njira yachiwiri.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 1mL ya saline wamba ku precipitate mutatsuka ndi saline wamba, kenako sakanizani bwino. Centrifuge pa 13,000r/min kwa mphindi 5, chotsani supernatant (sungani 10-20µL ya supernatant), ndikutsatira malangizo oti muchotse pambuyo pake.
Chothandizira kuchotsa chomwe chikulangizidwa: Nucleic Acid Extraction or Purification Reagent (YDP302) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd. Kuchotsa kuyenera kuchitika motsatira gawo lachiwiri la buku la malangizo. Ndikofunikira kugwiritsa ntchito madzi opanda RNase ndi DNase kuti muchotse madzi okhala ndi voliyumu ya 100µL.
