Tizilombo Tisanu ndi Timodzi Tomwe Timapuma
Dzina la chinthu
HWTS-RT175-Chida Chodziwira Matenda Asanu ndi Chimodzi a Nucleic Acid (Fluorescence PCR)
Epidemiology
Matenda opatsirana popuma ndi gulu lofala kwambiri la matenda a anthu lomwe lingathe kuchitika pa msinkhu uliwonse, zaka, ndi dera lililonse ndipo ndi chimodzi mwa zifukwa zazikulu zoyambitsa matenda ndi imfa mwa anthu padziko lonse lapansi. Matenda opatsirana popuma omwe amapezeka kawirikawiri ndi kachilombo koyambitsa matenda opatsirana popuma, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) ndi Mycoplasma pneumoniae. Zizindikiro zachipatala zomwe zimayambitsidwa ndi matenda opatsirana popuma zimafanana, koma chithandizo, mphamvu, ndi nthawi ya matendawa zimasiyana malinga ndi matenda opatsirana popuma. Pakadali pano, njira zazikulu zodziwira matenda opatsirana popuma omwe ali pamwambapa ndi awa: kudzipatula kwa kachilombo, kuzindikira ma antigen ndi kuzindikira nucleic acid. Kit iyi imathandiza kuzindikira matenda opatsirana popuma pozindikira ndi kuzindikira ma nucleic acid enaake mwa anthu omwe ali ndi zizindikiro za matenda opatsirana popuma, kuphatikiza ndi zina zomwe zapezeka kuchipatala ndi m'ma labotale.
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chitsanzo cha swab ya m'kamwa |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | LoD ya Adv, MP, RSV, hMPV, RhV ndi PIV zonse ndi 200Copies/mL. |
| Kufotokozera Mwapadera | Zotsatira za mayeso a cross-reactivity zinasonyeza kuti palibe cross-reactivity pakati pa kit ndi novel coronavirus, fuluwenza A virus, fuluwenza B virus, human bocavirus, cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, EBV, pertussis bacillus, Chlamydophila pneumoniae, Corynebacterium spp, Escherichia coli, Haemophilus influenzae, Lactobacillus spp, Legionella pneumophila, C. catarrhalis, ndi attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus salivarius, Actinobacillus baumannii, Narrow-feeding maltophilic monococci, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspergillus fumigatus, Aspergillus flavus, Pneumatobacteria spp, Candida albicans, Rohypnogonia viscera, oral streptococci, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia q fever ndi human genomic nucleic acids. Mphamvu yoletsa kusokoneza: mucin (60 mg/mL), magazi a munthu, benfotiamine (2 mg/mL), oxymetazoline (2 mg/mL), sodium chloride (20 mg/mL), beclomethasone (20 mg/mL), dexamethasone (20 mg/mL), flunitrazolone (20 μg/mL), triamcinolone acetonide (2 mg/mL), budesonide (1 mg/mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), katemera wa intranasal live flu virus, benzocaine (10%), menthol (10%), zanamivir (20 mg/mL), ribavirin (10 mg/L), paramivir (1 mg/mL), oseltamivir (0.15 mg/mL), mupirocin (20 mg/mL), tobramycin (0.6) mg/mL), UTM, saline, guanidine hydrochloride (5 M/L), Tris (2 M/L), ENTA-2Na (0.6 M/L), trilostane (15%), isopropyl alcohol (20%), ndi potassium chloride (1 M/L) adayesedwa kuti asokonezedwe, zotsatira zake zidawonetsa kuti panalibe kusokoneza komwe kudachitika chifukwa cha kupezeka kwa tizilombo toyambitsa matenda pa kuchuluka kwa zinthu zomwe zasokoneza zomwe zili pamwambapa. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la PCR la Applied Biosystems 7500 Real-Time QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. amalangizidwa kuti atenge zitsanzo ndinjira zotsatirazi ziyenera kutsatiridwamayendedweyakhazikitsidwa motsatira malamulo a IFUya Kit.
