Clostridium difficile poizoni A/B jini (C.diff)
Dzina la malonda
HWTS-OT031A Nucleic Acid Detection Kit for Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Clostridium difficile (CD), gram-positive anaerobic sporogenic Clostridium difficile, ndi imodzi mwa tizilombo toyambitsa matenda timene timayambitsa matenda a m'mimba.Zachipatala, pafupifupi 15% ~ 25% ya matenda otsekula m'mimba okhudzana ndi antimicrobial, 50% ~ 75% a antimicrobial-related colitis ndi 95% ~ 100% ya pseudomembranous enteritis amayamba ndi matenda a Clostridium difficile (CDI).Clostridium difficile ndi tizilombo toyambitsa matenda, kuphatikizapo tizilombo toyambitsa matenda komanso tizilombo toyambitsa matenda.
Channel
| FAM | tcdAjini |
| Mtengo ROX | tcdB ndijini |
| VIC/HEX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | chopondapo |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Mwatsatanetsatane | gwiritsani ntchito chida ichi kuti muzindikire tizilombo toyambitsa matenda a m'mimba monga Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Gulu B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, fuluwenza, fuluwenza virus, ndi fuluwenza DNA, zotsatira zake zonse ndi zoipa. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Hangzhouukadaulo wa bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Onjezani 180μL ya lysozyme buffer ku precipitate (kuchepetsa lysozyme ku 20mg/mL ndi lysozyme diluent), pipette kusakaniza bwino, ndi kukonza pa 37 ° C kwa mphindi zoposa 30. Tengani 1.5mL ya RNase / DNase-free centrifuge chubu, ndi kuwonjezera180μL ya kuwongolera kwabwino ndi kuwongolera koyipa motsatizana.Onjezani10μL yaulamuliro wamkati ku chitsanzo kuti chiyesedwe, kuwongolera koyenera, ndi kuwongolera koyipa motsatizana, ndikugwiritsa ntchito Nucleic Acid Extraction kapena Purification Reagent (YDP302) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd. chonde tsatirani mosamalitsa malangizo ogwiritsira ntchito masitepe enieni.Gwiritsani ntchito DNase/RNase yaulere ya H2O kuti asinthe, ndipo voliyumu yovomerezeka ndi 100μL.
Njira 2.
Tengani 1.5mL ya RNase/DNase-free centrifuge chubu, ndikuwonjezera 200μL ya kuwongolera koyenera ndi kuwongolera koyipa motsatizana.Onjezani10μL yoyang'anira mkati mwachitsanzo kuti chiyesedwe, kuwongolera koyenera, ndikuwongolera koyipa motsatizana, ndikugwiritsa ntchito Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito, ndipo voliyumu yovomerezeka ndi 80μL.
