Clostridium difficile toxin A/B gene (C.diff)
Dzina la chinthu
HWTS-OT031A Nucleic Acid Detection Kit ya Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Clostridium difficile (CD), yomwe ndi gram-positive anaerobic sporogenic Clostridium difficile, ndi imodzi mwa matenda akuluakulu omwe amayambitsa matenda a m'mimba a nosocomial. Malinga ndi zachipatala, pafupifupi 15% ~ 25% ya matenda otsegula m'mimba okhudzana ndi maantibayotiki, 50% ~ 75% ya matenda otupa omwe amabwera chifukwa cha maantibayotiki ndi 95% ~ 100% ya matenda a pseudomembranous enteritis amayamba chifukwa cha matenda a Clostridium difficile (CDI). Clostridium difficile ndi matenda omwe amabwera chifukwa cha mabakiteriya, kuphatikizapo mitundu ya poizoni ndi mitundu yosayambitsa poizoni.
Njira
| FAM | tcdAjini |
| ROX | tcdBjini |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | mpando |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Kufotokozera Mwapadera | Gwiritsani ntchito chida ichi kuti mupeze matenda ena a m'mimba monga Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Gulu B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus ndi human genomic DNA, zotsatira zake zonse ndi zoipa. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR (FQD-96A，HangzhouUkadaulo wa Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Onjezani 180μL ya lysozyme buffer ku precipitate (chepetsani lysozyme kufika pa 20mg/mL ndi lysozyme diluent), pipette kuti isakanizike bwino, ndikuikonza pa 37°C kwa mphindi zoposa 30. Tengani 1.5mL ya chubu cha RNase/DNase-free centrifuge, ndikuwonjezera180μL ya ulamuliro wabwino ndi ulamuliro woipa motsatizana. Onjezani10μL ya ulamuliro wamkati ku chitsanzo chomwe chiyenera kuyesedwa, ulamuliro wabwino, ndi ulamuliro woipa motsatizana, ndikugwiritsa ntchito Nucleic Acid Extraction kapena Purification Reagent (YDP302) ya Tiangen Biotech (Beijing) Co., Ltd. kuti mupeze chitsanzo china cha DNA, ndipo chonde tsatirani malangizo ogwiritsira ntchito pazinthu zinazake. Gwiritsani ntchito DNase/RNase free H2O ya elution, ndipo voliyumu yovomerezeka ya elution ndi 100μL.
Njira yachiwiri.
Tengani 1.5mL ya chubu cha RNase/DNase chopanda centrifuge, ndipo onjezerani 200μL ya positive control ndi negative control motsatizana.10μL ya ulamuliro wamkati ku chitsanzo chomwe chiyenera kuyesedwa, ulamuliro wabwino, ndi ulamuliro woipa motsatizana, ndikugwiritsa ntchito Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito, ndipo kuchuluka koyenera kwa elution ndi 80μL.
