Clostridium Hadul Toxin A / B Gene (C.Diff)
Dzina lazogulitsa
HWTS-OT031A Nucleuc Acid Resoct Kit for Clostridium Hardic Mexin A / B Gene (C.Wiff) (F.Diff)
Chiphaso
CE
Epidemiology
Clostridium Hardile (CD), Gram-Harsobic Sporogenic Clostridium Hastridium, ndi amodzi mwa tizilombo tating'onoting'ono toyambitsa matenda am'matumbo. Zachipatala, pafupifupi 15% ~ 25% ya diarrhea yaying'ono-25% ~ 75% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% ~ 100% Clostridium Hardile ndi pathogen yopanda matenda, kuphatikizapo ma toitaliniki ndi mavuto osadzinamiza.
Ngalande
| Banja | tcdafangano |
| Rox | tcdbfangano |
| Vic / Hex | Kuwongolera kwamkati |
Magawo aluso
| Kusunga | ≤-18 ℃ |
| Alumali-Moyo | Miyezi 12 |
| Mtundu wofanana | chopingasa |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lodu | 200Cfu / ml |
| Chifanizo | gwiritsani ntchito izi kuti muzindikire tizilombo toyambitsa matenda a Escherlia coli, Staphylococcus Aureus, Shigella, Vibridium, fuluwerza, fuluwenza B virus ndi fuko la anthu DNA, zotsatira zake ndi zonse zoipa. |
| Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Makupala®5 pcr yell pcr Slan-96p Real PCR Systems (Hongshi Chaurchirol Technology Co., Ltd.) Kuwala®480 pcr pcr system Stonegene 9600 kuphatikiza njira zenizeni za PCR yowunikira (Fqd-96a,HangizhouUkadaulo wa Bioer) Ma-6000 enieni ophatikizira cycler (Suzhou Molarray Colarray Colary Colarray Colary Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Cor Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Njira 1.
Onjezani 180μl ya lysozyme buffer kupita ku lysozyme mpaka 20mg / ml ya lysozyme arrifuge chubu, ndi kuwonjezera180μl ya kuwongolera bwino komanso kuwongolera koyenera. Onjeza10μL yamkati kwa zitsanzo kuti ziyesedwe, kuwongolera, komanso kuwongolera, ndikugwiritsa ntchito ma encletic acid (ydp302) poyambiranso, ndipo Chonde tsatirani malangizowo ogwiritsira ntchito njira zina. Gwiritsani ntchito DNASE / RANASE Free H2O Kwa elide, ndi mafashoni ogulitsa ndi 100μ.
Njira 2.
Tengani 1.5ml ya rnase / DNASE-Free Centrifuge chubu, ndikuwonjezera 200μl ya kuwongolera bwino komanso kuwongolera kotsatira. Onjeza10μL yamkati kwa zitsanzo kuti ziyesedwe, kuwongolera koyenera, komanso kuwongolera kotsatira, ndikugwiritsa ntchito ma viru a Macro & RNA-3004-48, 3004- 96) ndi macro & micro-micro-ma screc toctic acid exctor (hwts-3006). Kuchotsa kuyenera kuchitika molingana ndi malangizo ogwiritsira ntchito, ndipo kuchuluka kwa Evest Evel ndi 80μl.
