—— Kafukufuku Wogwirizana ndi Zhejiang CDC, Macro & Micro-Test, ndi China CDC Yofalitsidwa mu Frontiers in Cellular and Infection Microbiology
Chidule cha Phunziro
Mu Meyi 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) idasindikiza pepala lotsogozedwa ndi Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), ndi gulu la bioinformatics lochokera ku Beijing Macro & Micro-Test Bio-Tech Co., Ltd. ndi National Institute for Communicable Disease Control and Prevention (China CDC) ngati olemba anzawo. Kafukufukuyu ali ndi mutu wakuti:
"Kuzindikira ndi kusanthula kwa phylogenetic kwa mitundu isanu ndi iwiri ya Brucella abortus ku Zhejiang, China."
Kafukufukuyu akuyimira kusanthula koyamba kokhazikika, kochokera ku genome-based phylogenetic traceability kwa Brucella abortus (B. abortus) ku Zhejiang Province, China. Gululi linasanthula mitundu isanu ndi iwiri ya tizilombo tomwe tinasonkhanitsidwa kuyambira 2015 mpaka 2025 (mitundu inayi yochokera kwa anthu ndi mitundu itatu yochokera ku ng'ombe kuchokera ku Jinhua, Quzhou, ndi Ningbo). Zomwe zapezekazi zikupereka umboni wa majini wa chiyambi ndi njira zofalitsira za "mtundu waukulu wa kumpoto" uwu m'dera la mliri wakum'mwera chakum'mawa kwa China.
Mbiri ndi Kufunika Kwake
Brucellosis ndi matenda a ana omwe amayamba chifukwa cha mabakiteriya a mtundu wa Brucella. Brucella abortus imagwira makamaka ng'ombe koma ingayambitsenso matenda mwa anthu. Ku China, brucellosis imasonyeza kusiyana kwakukulu kwa malo: kuchuluka kwambiri kumachitika m'zigawo zakumpoto (monga Inner Mongolia, Shanxi, Heilongjiang). Mosiyana ndi zimenezi, madera akum'mwera, kuphatikizapo Zhejiang, akhala akulamulidwa ndi Brucella melitensis, ndipo milandu yochepa kwambiri ya B. abortus yanenedwa. Kusiyana kumeneku kwa madera kumapangitsa kuti kufotokozedwa kwa majini ndi komwe B. abortus imachokera ku Zhejiang kukhale kofunika kwambiri pa thanzi la anthu.
Njira ndi Zofunikira Zopezera
Gulu lofufuza linagwiritsa ntchito njira zosiyanasiyana zophatikiza sayansi ya zamoyo ndi sayansi ya zamoyo:
1.Kuzindikira tizilombo toyambitsa matenda ndi kulemba koyambira
BCSP-31 gene PCR ndi AMOS-PCR zatsimikizira kuti ma isolates onse asanu ndi awiri anali B. abortus.
Kulemba kwa Multilocus sequence (MLST) pogwiritsa ntchito majini asanu ndi anayi osamalira ana kunawonetsa kuti mitundu yonse ya majeremusi inali ya mtundu wa ST2, zomwe zikusonyeza kuti pali kufanana kwakukulu pakati pa mitundu ya B. abortus yomwe imafalikira ku Zhejiang.
2.Kufotokozera kwa majini onse
Kusanthula kwa majini onse kunachitika pa nsanja ya Illumina NovaSeq. Kusanthula kwapakati kwa nucleotide identity (ANI) kunawonetsa kuti ma isolate a Zhejiang anali ofanana mpaka 99.99% ndi mtundu wa B. abortus 544.
Kusanthula kwa ma genome kunavumbula kuti panali anthu osungidwa bwino kwambiri: majini 3,084 apakati adapezeka, pamodzi ndi majini 10 okha a chipolopolo, ndipo palibe majini ofewa apakati kapena amtambo omwe adapezeka.
3.Mbiri ya majini olimbana ndi kachilombo ndi maantibayotiki
Zinthu 68 zokhudzana ndi kachilomboka zinanenedweratu, zomwe zikuphatikizapo njira zakale monga LPS biosynthesis, T4SS secretion system, ndi BvrR-BvrS two-component regulatory system. Chodziwika bwino n'chakuti, mitundu yonse ya maselo inalibe majini a adhesin bmaA ndi btaF. Kusanthula kwa majini okana kunapeza jini la mprF lokha mu CARD database, popanda zizindikiro zina zokana zomwe zinapezeka.
4. Kukonzanso kwa phylogenetic ndi kutsatira kufalikira kwa kachilomboka
Kusanthula kwa Core-genome single-nucleotide polymorphism (cgSNP) kunayika ma isolate a Zhejiang pamalo enaake mu mtengo wapadziko lonse wa phylogenetic. Zotsatira zake zinasonyeza kuti mitundu ya Zhejiang imapanga gulu la monophyletic pamodzi ndi mitundu yochokera ku Russia, Mongolia, ndi madera angapo akumpoto kwa China (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Gululi linagawikanso m'magulu atatu osiyana (Clade 1-3), zomwe zikusonyeza zochitika zingapo zodziyimira pawokha.
Mapeto ndi Zotsatira zake
Kafukufukuyu akupereka deta yoyamba yolondola kwambiri ya majini ya B. abortus ku Zhejiang Province ndipo akupereka mfundo zingapo zofunika:
- Clear maziko a majini– Mitundu ya B. abortus yomwe imapezeka ku Zhejiang ndi ya ST2, imasungidwa bwino kwambiri m'majini, ndipo imayimira mtundu wamba wa brucellosis ya ng'ombe.
2. Evidence of cross regional transmission– Kusanthula kwa phylogenetic sikutsimikizira kuti pali mzere wodziyimira pawokha ku Zhejiang. M'malo mwake, deta ikusonyeza kuti mitundu iyi inachokera kumpoto kwa China ndipo ikhoza kukhala ndi mbiri yofanana ya kusintha kwa zinthu ndi mitundu yochokera ku Russia ndi Mongolia. Kupezeka kwa magulu atatu ang'onoang'ono kumatanthauza zochitika zingapo zosiyana zoyambira.
3. Zotsatira za thanzi la anthu- Zomwe zapezekazi zikugogomezera kufunika kwa kuyang'anira matenda a brucellosis m'majini ngakhale m'madera omwe nthawi zambiri samapezeka kwambiri monga Zhejiang. Ngakhale kuti chiwerengero cha milandu yomwe ilipo pano chili chochepa, zida zodziwika bwino monga cgSNP zimatha kupeza komwe kumachokera matenda ochokera kunja ndikupereka umboni wasayansi kuti usokoneze unyolo wofalitsa matenda okhudzana ndi mayendedwe a ziweto pakati pa madera.
Ntchitoyi sikuti imangodzaza mpata wofufuza m'chigawo cha Zhejiang komanso imapereka deta yatsopano yoyambira yowunikira tizilombo toyambitsa matenda komanso kuwunika zoopsa za brucellosis m'chigawo cha Yangtze River Delta.
Zambiri za Pepala:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Kuzindikira ndi kusanthula kwa phylogenetic kwa mitundu isanu ndi iwiri ya Brucella abortus ku Zhejiang, China. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Nthawi yotumizira: Juni-10-2026