Mycoplasma Pneumoniae (MP)
Dzina la malonda
HWTS-RT024 Mycoplasma Pneumoniae (MP) Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) ndi mtundu wa tizilombo tating'onoting'ono ta prokaryotic, tomwe tili pakati pa mabakiteriya ndi ma virus, okhala ndi ma cell koma opanda khoma la cell. MP makamaka imayambitsa matenda amtundu wa kupuma kwa anthu, makamaka kwa ana ndi achinyamata. Zingayambitse anthu mycoplasma chibayo, ana kupuma thirakiti matenda ndi atypical chibayo. The matenda mawonetseredwe zosiyanasiyana, ambiri amene ali kwambiri chifuwa, malungo, kuzizira, mutu, zilonda zapakhosi. Matenda a m'mwamba ndi chibayo cha bronchial ndizofala kwambiri. Odwala ena amatha kukhala ndi matenda am'mwamba kupita ku chibayo chachikulu, kupuma movutikira komanso kufa.
Channel
| FAM | Mycoplasma chibayo |
| VIC/HEX | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Makopi / ml |
| Mwatsatanetsatane | a） Cross reactivity: palibe mtanda reactivity ndi Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriudomococcus tuberlamonase aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. b) Kuthekera koletsa kusokoneza: palibe kusokoneza pamene zinthu zosokoneza zinayesedwa ndi zotsatirazi: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) magazi amunthu, levofloxacin (10μg/mL), moxiflogxacin/0. azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
(1) Chitsanzo cha Sputum
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid (CTS) Extra3006W HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200µL ya saline wamba pa mafunde okonzedwa. Wotsatira m'zigawo ayenera kuchitidwa molingana ndi malangizo ntchito. Voliyumu yovomerezeka ya elution ndi 80µL. Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP315-R). The m'zigawo ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito. Voliyumu yovomerezeka ndi 60µL.
(2) Mphuno ya Oropharyngeal
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid (CTS) Extra3006W HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The analimbikitsa m'zigawo voliyumu ya chitsanzo ndi 200µL, ndipo analimbikitsa elution voliyumu ndi 80µL. The m'zigawo ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito. Voliyumu yotulutsa yovomerezeka ndi 140µL, ndipo voliyumu yovomerezeka ndi 60µL.
