Mycoplasma Pneumoniae (MP)
Dzina la chinthu
HWTS-RT024 Mycoplasma Pneumoniae（MP） Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) ndi mtundu wa tizilombo tating'onoting'ono ta prokaryotic, tomwe tili pakati pa mabakiteriya ndi kachilombo, ndipo tili ndi kapangidwe ka maselo koma tilibe khoma la maselo. MP imayambitsa matenda opatsirana m'njira yopumira mwa anthu, makamaka mwa ana ndi achinyamata. Ingayambitse chibayo cha mycoplasma mwa anthu, matenda opatsirana m'njira yopumira mwa ana komanso chibayo chosazolowereka. Zizindikiro zake ndi zosiyanasiyana, zomwe zambiri ndi chifuwa chachikulu, malungo, kuzizira, mutu, kupweteka pakhosi. Matenda opatsirana m'njira yopumira m'mwamba ndi chibayo cha bronchial ndi omwe amapezeka kwambiri. Odwala ena amatha kudwala matenda opatsirana m'njira yopumira m'mwamba mpaka chibayo chachikulu, kupuma movutikira kwambiri komanso imfa zimatha kuchitika.
Njira
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chiphuphu, swab ya m'kamwa |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makope 200/mL |
| Kufotokozera Mwapadera | a） Kuyanjana kwa mitundu yosiyanasiyana: palibe kuyanjana kwa mitundu yosiyanasiyana ndi Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus ndi human genomic nucleic acid. b）Kutha kuletsa kusokoneza: palibe kusokoneza pamene zinthu zosokonezazo zinayesedwa ndi kuchuluka kotsatira: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) magazi a munthu, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
(1) Chitsanzo cha Mafinya
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200µL ya saline wamba ku precipitate yokonzedwa. Kuchotsa kotsatira kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Voliyumu yovomerezeka ya elution ndi 80µL. Chothandizira kuchotsa chomwe chikulangizidwa: Nucleic Acid Extraction kapena Purification Reagent (YDP315-R). Kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Voliyumu yovomerezeka ya elution ndi 60µL.
(2) Chimbudzi cha m'kamwa
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo komwe kukulangizidwa ndi 200µL, ndipo kuchuluka kwa elution komwe kukulangizidwa ndi 80µL. Chothandizira kuchotsa chomwe chikulangizidwa: QIAamp Viral RNA Mini Kit (52904) kapena Nucleic Acid Extraction or Purification Reagent (YDP315-R). Kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chomwe chimaperekedwa ndi 140µL, ndipo kuchuluka kwa chotsukira chomwe chimaperekedwa ndi 60µL.
