Matenda a Kupuma ku Middle East Coronavirus Nucleic Acid
Dzina la chinthu
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (Fluorescent PCR)
Epidemiology
Matenda a coronavirus a ku Middle East (MERS-CoV), kachilombo ka β-coronavirus komwe kamayambitsaMatenda a kupuma mwa anthu, adapezeka koyamba mwa wodwala wamwamuna wazaka 60 waku Saudi Arabia yemwe anamwalira pa 24 Julayi, 2012. Kuwonetsedwa kwa matenda a MERS-CoV kumayambira pa mkhalidwe wopanda zizindikiro kapena zizindikiro zochepa za kupuma mpaka matenda oopsa a kupuma ngakhale imfa.
Njira
| FAM | RNA ya kachilombo ka MERS |
| VIC(HEX) | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ Mumdima |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Ma swab a m'mphuno omwe asonkhanitsidwa kumene |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | Makope 1000/mL |
| Kufotokozera Mwapadera | Palibe njira yolumikizirana pakati pa ma coronavirus a anthu ndi ma coronavirus a anthu SARSr-CoV ndi matenda ena ofala. |
| Zida Zogwiritsidwa Ntchito: | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Chothandizira kuchotsa chomwe chikulangizidwa: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction kapena Purification Reagent (YDP315-R) yochokera ku Tiangen Biotech (Beijing) Co., Ltd.
Njira yachiwiri.
Chotsukira chomwe chikulangizidwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Magulu a zinthu
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