KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Dzina la chinthu
KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Epidemiology
Kusintha kwa mfundo mu jini ya KRAS kwapezeka m'mitundu ingapo ya khansa ya anthu, pafupifupi 17% ~ 25% ya kusintha kwa khansa, 15% ~ 30% ya kusintha kwa khansa ya m'mapapo, 20% ~ 50% ya kusintha kwa khansa ya m'matumbo. Chifukwa puloteni ya P21 yomwe imalembedwa ndi jini ya K-ras ili pansi pa njira yolumikizirana ya EGFR, pambuyo pa kusintha kwa jini ya K-ras, njira yolumikizirana yotsika nthawi zonse imayendetsedwa ndipo sikhudzidwa ndi mankhwala otsogola pa EGFR, zomwe zimapangitsa kuti maselo achuluke mosalekeza. Kusintha kwa jini ya K-ras nthawi zambiri kumapereka kukana kwa EGFR tyrosine kinase inhibitors mwa odwala khansa ya m'mapapo komanso kukana mankhwala oletsa anti-EGFR antibody mwa odwala khansa ya m'matumbo [1, 2, 3]. Mu 2008, National Comprehensive Cancer Network (NCCN) idapereka malangizo azachipatala okhudza khansa ya m'matumbo, omwe adanenanso kuti malo omwe amachititsa kuti K-ras iyambe kugwira ntchito amapezeka makamaka mu codons 12 ndi 13 ya exon 2, ndipo adalimbikitsa kuti odwala onse omwe ali ndi khansa ya m'matumbo yofalikira kwambiri athe kuyesedwa kusintha kwa K-ras asanalandire chithandizo [4]. Chifukwa chake, kuzindikira mwachangu komanso molondola kwa kusintha kwa majini a K-ras ndikofunikira kwambiri pakuwongolera mankhwala azachipatala. Kit iyi imagwiritsa ntchito DNA ngati chitsanzo chowunikira kuti ipereke kuwunika koyenera kwa momwe munthu alili ndi kusintha kwa majini, komwe kungathandize asing'anga pofufuza khansa ya m'matumbo, khansa ya m'mapapo ndi odwala ena otupa omwe amapindula ndi mankhwala omwe akufuna. Zotsatira za mayeso a kit ndi zachipatala zokha ndipo siziyenera kugwiritsidwa ntchito ngati maziko okhawo ochizira odwala payekha. Madokotala ayenera kupanga zigamulo zonse pazotsatira za mayeso kutengera zinthu monga momwe wodwalayo alili, zizindikiro za mankhwala, momwe chithandizo chimayankhidwira ndi zizindikiro zina zoyesera za labotale.
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | magawo a matenda omwe amaphatikizidwa ndi parafini mwa anthu |
| CV | ≤5.0% |
| LoD | a) K-ras Reaction Buffer A ndi K-ras Reaction Buffer B amatha kuzindikira mosavuta 1% ya kusintha kwa 3ng/μL pansi pa mtundu wa wild-type background; b) Kusintha kwa 1×103Makopi/mL amatha kupezeka mokhazikika kumbuyo kwa 1 × 105Makopi/mL pamene chiŵerengero cha kusintha kwa majini chili 1%; c) Pamene kampani ya LoD reference SW3 yayesedwa, palibe Ct value kapena Ct value=0 ya Reaction Buffer A ndi Reaction Buffer B |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500 Machitidwe a PCR Ogwiritsidwa Ntchito Nthawi Yeniyeni, Machitidwe a Biosystems Ogwiritsidwa Ntchito 7300 Machitidwe a PCR Ogwiritsidwa Ntchito Nthawi Yeniyeni, QuantStudio®5 Machitidwe a PCR Ogwiritsidwa Ntchito Nthawi Yeniyeni, Dongosolo la LightCycler® 480 la PCR Yogwiritsidwa Ntchito Nthawi Yeniyeni, Dongosolo la BioRad CFX96 la PCR Yogwiritsidwa Ntchito Nthawi Yeniyeni. |
Kuyenda kwa Ntchito
Reagent ikufunika koma siinaperekedwe:DNase/RNase yopanda H2O, ethanol yopanda madzi. Poyesa chitsanzo cha minofu yolumikizidwa ndi paraffin, ndi bwino kugwiritsa ntchito QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) ndi Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd.
Zinthu zofunika koma zosaperekedwa:Malangizo opanda DNase/RNase, magolovesi otayidwa, chubu cha Centrifuge chopanda DNase/RNase, mipiringidzo ya machubu 8, centrifuge.
