Kiti Yodziwira Matenda a Dengue I/II/III/IV (Fluorescence PCR)-RUO youma ndi yozizira
Dzina la chinthu
Kiti Yodziwira Matenda a Dengue I/II/III/IV (Fluorescence PCR)-RUO youma ndi yozizira
Epidemiology
Matenda a Dengue Fever (DF), omwe amayamba chifukwa cha matenda a denguevirus (DENV), ndi amodzi mwa matenda opatsirana a arbovirus omwe amafalikira kwambiri. Njira yake yofalitsira matendawa imaphatikizapo Aedes aegypti ndi Aedes albopictus. Matenda a DF amapezeka kwambiri m'madera otentha komanso otentha. DENV ndi ya flavivirus yomwe ili pansi pa flaviviridae, ndipo imatha kugawidwa m'magulu anayi malinga ndi antigen pamwamba. Zizindikiro zachipatala za matenda a DENV zimaphatikizapo mutu, malungo, kufooka, kukulira kwa lymph node, leukopenia ndi zina zotero, komanso kutuluka magazi, kugwedezeka, kuvulala kwa chiwindi kapena imfa m'milandu yoopsa. M'zaka zaposachedwa, kusintha kwa nyengo, kufalikira kwa mizinda, chitukuko chachangu cha zokopa alendo ndi zinthu zina zapereka mikhalidwe yachangu komanso yosavuta yofalitsira ndi kufalikira kwa DF, zomwe zimapangitsa kuti dera la mliri wa DF lipitirire kukula. Chifukwa chake, kukhazikitsidwa kwa njira yosavuta, yeniyeni komanso yachangu yodziwira matenda a DENV ndikofunikira kwambiri pakuzindikira matenda a DF.
Magawo aukadaulo
| Malo Osungirako | 2-8℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | seramu |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Makope 500/mL |
| Kufotokozera Mwapadera | Mayeso ndi oipa pa zitsanzo za seramu ya dengue virus negative; zotsatira za mayeso osokoneza zimasonyeza kuti pamene kuchuluka kwa bilirubin mu seramu sikupitirira 168.2μmol/mL, kuchuluka kwa hemoglobin komwe kumapangidwa ndi hemolysis sikupitirira 130g/L, kuchuluka kwa mafuta m'magazi sikupitirira 65mmol/mL, ndipo kuchuluka konse kwa IgG mu seramu sikupitirira 5mg/mL, palibe chomwe chimapangitsa kuti kachilombo ka dengue kapezeke; gwiritsani ntchito kachilombo ka Hepatitis A, kachilombo ka Hepatitis B, kachilombo ka Hepatitis C, kachilombo ka Herpes, kachilombo ka Eastern equine encephalitis, kachilombo ka Hantaan, kachilombo ka Bunia, kachilombo ka West Nile, kachilombo ka Zika ndi zitsanzo za seramu ya anthu kuti muyesedwe mosiyanasiyana, ndipo zotsatira zake zikusonyeza kuti palibe kusinthana pakati pa zida izi ndi tizilombo toyambitsa matenda pamwambapa. |
| Zida Zogwiritsidwa Ntchito | Mtundu Woyamba: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Mtundu Wachiwiri: EudemonTMAIO800 (HWTS-EQ007) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Kuyenda kwa Ntchito
Ma reagents amafunika koma osaperekedwa:
Mtundu Woyamba: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Mtundu Wachiwiri: Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), ingagwiritsidwe ntchito ndi EudemonTM AIO800 (HWTS-EQ007) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Musanagwiritse ntchito Type II test reagent, kasitomala ali ndi reagent yothandizira kuchotsa ndi chida chowonjezera, ndipo mainjiniya adzatsogolera kasitomala kuti achite ntchito yoyesera akagwiritsa ntchito koyamba.
Zinthu zogwiritsidwa ntchito zimafunika koma siziperekedwa:Malangizo a DNase/RNase Free, magolovesi otayidwa, machubu a DNase/RNase Free EP, centrifuge, choyimilira cha maginito, mipiringidzo ya machubu 8 a PCR.
