Kiti Yodziwira Kachilombo ka Ebola ya Bundybugyo (Fluorescence PCR)
Dzina la chinthu
Kiti Yodziwira Kachilombo ka Ebola ya Bundybugyo (Fluorescence PCR)
Epidemiology
Filoviridae. Ndi kachilombo kachitatu ka Ebola komwe kangayambitse miliri yayikulu kwa anthu,pambuyo pa Zaire ebolavirus (EBOV) ndi Sudan ebolavirus (SUDV). BDBV ndi kachilombo kamodzi kokhakachilombo ka RNA kamene kali ndi strand yoipa yokhala ndi genome ya pafupifupi 19 kb, yokhala ndi ma encoding 7kapangidwe ka zinthumapuloteni ndi mapuloteni awiri osapangidwa. Nthawi yoberekera matenda a BDBV ndi 2–21masiku (avereji ya masiku 6.3). Zizindikiro zake zimafanana kwambiri ndi za mavairasi ena a Ebolandipo sizingasiyanitsidwe ndi zizindikiro zachipatala zokha. Mu Ogasiti 2007,Kufalikira kwa matenda otupa magazi m'thupi mosadziŵika bwino kunachitika m'boma la Bundibugyo kuKumadzulo kwa Uganda. Chinadziwika kuti ndi kachilombo katsopano ka Ebola ndipo chinatchedwa Bundibugyoebolavirus (BDBV). Panali milandu 93 yomwe ikukayikiridwa, milandu 56 yotsimikizika, ndi imfa 37(chiwerengero cha anthu omwe amwalira ndi 25%). Mu 2012, kufalikira kwina kunachitika ku Orientale Province yaDemocratic Republic of the Congo, yokhala ndi milandu 38 yotsimikizika ndi imfa 13 (imfa ya wodwala)chiwerengero cha 34%. Mu Meyi 2026, bungwe la WHO linalengeza za kufalikira kwa BDBV komwe kukuchitika panoDemocratic Republic of the Congo ndi Uganda ndi Zadzidzidzi pa Zaumoyo wa Anthu OnseInternational Concern (PHEIC). Pofika pa Meyi 20, panali milandu 246 yomwe ikukayikiridwa ndipo 80imfa.
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | 12miyezi |
| Mtundu wa Chitsanzo | magazi athunthu, seramu/plasma,oswab ya ral |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 3Makope 00/mL |
| Zida Zogwiritsidwa Ntchito | Mtundu Woyamba:Dongosolo la PCR la ABI 7500/7500 lachangu nthawi yeniyeni, Dongosolo la PCR la QuantStudio®5 Real-Time, Dongosolo la PCR la QuantStudio®7 Real-Time, Dongosolo Losanthula la PCR la Hongshi SLAN-96P/S Lodzipangira Lokha, Dongosolo la PCR la LightCycler®480 Real-Time, Hangzhou Bioer FQD-96A Real-Time PCR System, Yarui MA-6000 Real-Time PCR System, Dongosolo la BioRad CFX96 Real-Time PCR, BioRad CFX Opus 96 Real-Time PCR System. Mtundu Wachiwiri: Dongosolo Lodziwira Mamolekyulu a Eudemon AIO800 (HWTS-AIO800)
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Kayendedwe ka ntchito:
Mtundu Woyamba:
Mtundu Wachiwiri:
Zodzikonzekeretsa zokha: Saline ya thupi, ma reagents ochotsera nucleic acid
Zogwiritsidwa Ntchito Zodzikonzekeretsa: Ma pipette opanda DNase/RNase, magolovesi otayidwa, 1.5 mL
Machubu a centrifuge opanda DNase/RNase, centrifuge







