Klebsiella Pneumoniae, Acinetobacter Baumannii ndi Pseudomonas Aeruginosa ndi Drug Resistance Genes (KPC, NDM, OXA48 ndi IMP) Multiplex
Dzina la chinthu
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii ndi Pseudomonas Aeruginosa ndi Drug Resistance Genes (KPC, NDM, OXA48 ndi IMP) Multiplex Detection Kit (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Klebsiella pneumoniae ndi kachilombo kofala kwambiri komwe kamayambitsa matenda opatsirana m'thupi ndipo ndi kachilombo kofala kwambiri komwe kamayambitsa matenda a nosocomial. Pamene mphamvu ya thupi yachepa, mabakiteriya amalowa m'mapapo kuchokera m'njira yopumira, zomwe zimayambitsa matenda m'mbali zosiyanasiyana za thupi, ndipo kugwiritsa ntchito mankhwala opha tizilombo toyambitsa matenda koyambirira ndiye njira yochiritsira [1]. Malo ofala kwambiri a matenda a Acinetobacter baumannii ndi mapapo, omwe ndi kachilombo kofunikira kwambiri pa matenda a chibayo chopezeka kuchipatala (HAP), makamaka chibayo chogwirizana ndi Ventilator (VAP). Nthawi zambiri amatsagana ndi matenda ena a bakiteriya ndi bowa, omwe ali ndi zizindikiro za kuchuluka kwa matenda komanso kuchuluka kwa imfa. Pseudomonas aeruginosa ndi kachilombo kofala kwambiri ka gram-negative bacilli m'machitidwe azachipatala, ndipo ndi kachilombo kofunikira kwambiri pa matenda opatsirana m'chipatala, komwe kumakhala kosavuta kulowa m'thupi, kusiyana kosavuta komanso kukana mankhwala ambiri.
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Kutupa kwa mbolo |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | Makopi 1000/mL |
| Kufotokozera Mwapadera | a) Mayeso a cross-reactivity akuwonetsa kuti kit iyi ilibe reactivity yofanana ndi matenda ena opatsirana popuma, monga Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus ndi sputum samples zopanda zolinga, ndi zina zotero. b) Mphamvu yoletsa kusokoneza: Sankhani mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, ndi roxithromycin, ndi zina zotero. kuti muyese kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zosokoneza zomwe zatchulidwa pamwambapa sizimasokoneza kuzindikira majini a Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa ndi carbapenem resistance KPC, NDM, OXA48 ndi IMP. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500 Real-Time PCR, Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni, QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni, Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Dongosolo la BioRad CFX96 Real-Time PCR, BioRad CFX Opus 96 Real-Time PCR System. |
Kuyenda kwa Ntchito
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. imalimbikitsidwa pochotsa zitsanzo ndipo njira zotsatirazi ziyenera kuchitidwa motsatira IFU ya Kit.
