Mankhwala a Leukocyte Antigen B27 Nucleic Acid a Anthu
Dzina la chinthu
HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ankylosing spondylitis (AS) ndi matenda otupa omwe amafalikira kwambiri omwe amalowa msana ndipo amatha kukhudza ziwalo za sacroiliac ndi ziwalo zozungulira mosiyanasiyana. Zawululidwa kuti AS ikuwonetsa kusonkhana kwa mabanja ndipo imagwirizana kwambiri ndi antigen ya leukocyte ya munthu HLA-B27. Mwa anthu, mitundu yoposa 70 ya mitundu ya HLA-B27 yapezeka ndipo yadziwika, ndipo mwa iwo, HLA-B*2702, HLA-B*2704 ndi HLA-B*2705 ndi mitundu yodziwika kwambiri yokhudzana ndi matendawa. Ku China, Singapore, Japan ndi Taiwan district ku China, mtundu wodziwika kwambiri wa HLA-B27 ndi HLA-B*2704, womwe uli pafupifupi 54%, kutsatiridwa ndi HLA-B*2705, yomwe ili pafupifupi 41%. Kiti iyi imatha kuzindikira DNA m'magulu a HLA-B*2702, HLA-B*2704 ndi HLA-B*2705, koma siisiyanitsa pakati pawo.
Njira
| FAM | HLA-B27 |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | Madzi: ≤-18℃ Mumdima |
| Nthawi yokhalitsa | Madzi: miyezi 18 |
| Mtundu wa Chitsanzo | zitsanzo za magazi athunthu |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| Kufotokozera Mwapadera
| Zotsatira za mayeso zomwe zapezeka ndi kit iyi sizidzakhudzidwa ndi hemoglobin (<800g/L), bilirubin (<700μmol/L), ndi mafuta m'magazi/triglycerides (<7mmol/L) m'magazi. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a Biosystem Ogwiritsidwa Ntchito StepOne Real-Time PCR Systems Dongosolo la LightCycler®480 Real-Time PCR Dongosolo la Agilent-Stratagene Mx3000P Q-PCR |
