Enterovirus Universal
Dzina la chinthu
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a mkamwa mwa manja ndi matenda opatsirana omwe amayamba chifukwa cha ma enterovirus (EV). Pakadali pano, mitundu 108 ya ma enterovirus yapezeka, yomwe yagawidwa m'magulu anayi: A, B, C ndi D. Pakati pawo, enterovirus EV71 ndi CoxA16 ndi omwe amayambitsa matenda akuluakulu. Matendawa amapezeka kwambiri mwa ana osakwana zaka 5, ndipo angayambitse mabala m'manja, mapazi, pakamwa ndi ziwalo zina. Ana ochepa amakhala ndi mavuto monga myocarditis, pulmonary edema, ndi aseptic meningoencephalitis.
Njira
| FAM | EV RNA |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chimbudzi cha m'kamwa,Madzi a Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/mL |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500/7500 Machitidwe a PCR Ofulumira Nthawi Yeniyeni, QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Chida Chothandizira Kuchotsa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), iyenera kuchotsedwa motsatira malangizo. Voliyumu ya chitsanzo ndi 200 μL, voliyumu yovomerezeka ya elution ndi 80µL.
Njira yachiwiri.
Chida chotulutsira mankhwala chomwe chimalimbikitsidwa: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), chiyenera kuchotsedwa motsatira malangizo.
Njira 3.
Kiti yotulutsira yomwe ikulangizidwa: QIAamp Viral RNA Mini Kit (52904) kapena Nucleic Acid Extraction or Purification Kit (YDP315-R), iyenera kuchotsedwa motsatira malangizo. Kuchuluka kwa chitsanzo ndi 140 μL, kuchuluka kwa elution komwe kukulangizidwa ndi 60µL.
