Enterovirus Universal
Dzina la malonda
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a Hand-foot-mouth ndi matenda opatsirana omwe amayamba chifukwa cha enteroviruses (EV).Pakalipano, mitundu 108 ya serotypes ya enteroviruses yapezeka, yomwe imagawidwa m'magulu anayi: A, B, C ndi D. Pakati pawo, enterovirus EV71 ndi CoxA16 ndizo tizilombo toyambitsa matenda.Matendawa nthawi zambiri amapezeka mwa ana osakwana zaka 5, ndipo angayambitse nsungu pamanja, mapazi, pakamwa ndi mbali zina.Ana ochepa amatha kukhala ndi zovuta monga myocarditis, pulmonary edema, ndi aseptic meningoencephalitis.
Channel
| FAM | EV RNA |
| Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Oropharyngeal swab,Herpes madzimadzi |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi / ml |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500/7500 Fast Real-Time PCR Systems, Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Limbikitsani Zida Zotsitsa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), iyenera kuchotsedwa mosamalitsa motsatira malangizo.Voliyumu yachitsanzo ndi 200 μL, voliyumu yovomerezeka ndi 80µL.
Njira 2.
Zida zopangira zovomerezeka: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), ziyenera kuchotsedwa mosamalitsa malinga ndi malangizo.
Njira 3.
Zida zotsatsira zolangizidwa: QIAamp Viral RNA Mini Kit (52904) kapena Nucleic Acid Extraction or Purification Kit (YDP315-R), ziyenera kutulutsidwa motsatira malangizo.Voliyumu yachitsanzo ndi 140 μL, voliyumu yovomerezeka ndi 60µL.
