Kachilombo ka Ebola ku Zaire

Kufotokozera Kwachidule:

Kiti iyi ndi yoyenera kuzindikira bwino nucleic acid ya kachilombo ka Zaire Ebola mu seramu kapena plasma ya odwala omwe akuganiziridwa kuti ali ndi kachilombo ka Zaire Ebola (ZEBOV).

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Dzina la chinthu

HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Kachilombo ka Ebola ndi ka Filoviridae, komwe ndi kachilombo ka RNA kopanda magawo kamodzi. Mavairasi ndi ulusi wautali wokhala ndi kutalika kwa virion wapakati wa 1000nm ndi mainchesi pafupifupi 100nm. Jenomu ya kachilombo ka Ebola ndi RNA yopanda magawo yokhala ndi kukula kwa 18.9kb, yokhala ndi mapuloteni 7 omangira ndi puloteni imodzi yosakhala yomangira. Kachilombo ka Ebola kangagawidwe m'magulu monga Zaire, Sudan, Bundibugyo, Tai Forest ndi Reston. Pakati pawo, mtundu wa Zaire ndi mtundu wa Sudan zanenedwa kuti zimayambitsa imfa za anthu ambiri chifukwa cha matenda. EHF (Ebola Hemorrhagic Fever) ndi matenda opatsirana opatsirana omwe amayamba chifukwa cha kachilombo ka Ebola. Anthu amadwala kwambiri akakhudza madzi amthupi, madzi otuluka m'thupi ndi ndowe za odwala kapena nyama zomwe zili ndi kachilomboka, ndipo zizindikiro zake zimakhala malungo, kutuluka magazi komanso kuwonongeka kwa ziwalo zingapo. EHF ili ndi chiwerengero chachikulu cha imfa cha 50%-90%.

Njira

FAM	MP nucleic acid
ROX	Kulamulira Kwamkati

Magawo aukadaulo

Malo Osungirako	≤-18℃
Nthawi yokhalitsa	Miyezi 9
Mtundu wa Chitsanzo	Seramu watsopano, Plasma
Tt	≤38
CV	≤5.0%
LoD	Makopi 500/μL
Kufotokozera Mwapadera	Gwiritsani ntchito zidazi kuti muyese maumboni oipa a kampani, zotsatira zake zikukwaniritsa zofunikira.
Zida Zogwiritsidwa Ntchito	Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, ndi BioRad CFX Opus 96 Real-Time PCR System

Kuyenda kwa Ntchito

Njira 1.

Chotsukira chomwe chikulangizidwa: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) yochokera ku Tiangen Biotech (Beijing) Co., Ltd. Iyenera kuchotsedwa motsatira malangizo, ndipo kuchuluka kwa chitsanzo chomwe chikulangizidwa ndi 140μL ndipo kuchuluka kwa chotsukira chomwe chikulangizidwa ndi 60μL.

Njira yachiwiri.

Chotsukira chomwe chikufunidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Chiyenera kuchotsedwa motsatira malangizo. Voliyumu ya chitsanzo chotsukira ndi 200μL, ndipo voliyumu yovomerezeka ya elution ndi 80μL.

Njira 3.

Ma reagents ofunikira ochotsera: Nucleic Acid Extraction Reagent (1000020261) ndi High Throughput Automated Sample Preparation System (MGISP-960) ndi BGI ziyenera kuchotsedwa motsatira malangizo. Kuchuluka kwa kuchotsa ndi 160μL, ndipo kuchuluka kwa elution komwe kumalimbikitsidwa ndi 60μL.