Zaire Ebola
Dzina lazogulitsa
HWTS-Fe008 Zaire Ebola Virus Nucleic acid Pulogalamu (Fluorescence PCR)
Epidemiology
Ebola Virus ndi a filviviridae, yomwe ndi virus yosagwedezeka yopanda kanthu RNA. Mavairasi amatalika kwambiri kutalika kwa 1000nm ndi mainchesi pafupifupi 100nm. Mtundu wa Ebola Virus ndi RNA yosagwedezeka yokhala ndi kukula kwa 18.9kb, malo ojambula 7 ndi mapuloteni 1 osakhala makina. Ebola Virus ikhoza kugawidwa ngati Zaire, Sudan, Bundbugyo, nkhalango ndi reskon. Pakati pawo, mtundu wa Zaire ndi mtundu wa Sudan wanenedwa kuti athetse imfa ya anthu ambiri. EHF (Ebola wa hemorrhagic mankhwala matenda matenda otupa oyambitsidwa ndi matenda a Ebola. Anthu amatenga kachilomboka makamaka polumikizana ndi madzi amthupi, zotchinga ndi ziweto za odwala kapena ziweto zodwala, ndipo mawonetseredwe azachipatala amawononga malungo, kuwonongeka kwa magazi ndi ziwalo zingapo. EHF ili ndi zoopsa za 50% -90%.
Ngalande
| Banja | Mp nuclec acid |
| Rox | Kuwongolera kwamkati |
Magawo aluso
| Kusunga | ≤-18 ℃ |
| Alumali-Moyo | Miyezi 9 |
| Mtundu wofanana | Seramu watsopano, ma plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lodu | 500 makope / μl |
| Chifanizo | Gwiritsani ntchito zipamba kuti zisayesere mafotokozedwe a kampani, zotsatira zake zikwaniritse zofunika. |
| Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Ma pcr-5 a PCR Slan-96p Real PCR Systems (Hongshi Chaurchirol Technology Co., Ltd.) Speccycler®480 PCR System Stonegene 9600 kuphatikiza njira zenizeni za PCR (FQD-96A, maluso a bioriou bioer) Ma-6000 enieni ophatikizira cycler (Suzhou Molarray Colarray Colary Colarray Colary Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Cor Biorad Cfx96 PCRS PCR System, ndi Biorad Cfx Opos 96 Real PCR Systems |
Kuyenda
Njira 1.
Kulimbikitsidwa Kutulutsa: Qaamp Viral Rna Kit Kit (52904), ecleiction acid exagection kapena choyeretsa (ydp315-r), ltd. Iyenera kuperekedwa molingana ndi malangizo, ndipo mawu ovomerezeka a chitsanzo ndi 140μl ndi mafakitale olimbikitsa ndi 60μl.
Njira 2.
Kulimbikitsidwa Kwambiri: Macro & Bigro-Mati Kil DNA / RNTS (HWTS-3004-32, HWTS-3004-96) ndi Micro-3006). ziyenera kuchotsedwa molingana ndi malangizo. Buku la zitsanzo ndi 200μl, ndipo kuchuluka kwa Evest Evel ndi 80μl.
Njira 3.
Kulimbikitsidwa Kutulutsa: Nyukiliya ya nucleic acid Exprection (1000020261) ndi Kutulutsa Kwapamwamba Koyambira (Mgisp-960) Wolemba BGI iyenera kuchotsedwa molingana ndi malangizo. Buku la Kuzikuza ndi 160μl, ndi mafakitale olimbikitsidwa ndi 60.
