Zaire Ebola Virus
Dzina la malonda
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka Ebola ndi ka Filoviridae, kachirombo ka RNA kamene kali ndi chingwe chimodzi.Ma virus ndi ma filaments aatali okhala ndi kutalika kwa virion pafupifupi 1000nm ndi m'mimba mwake pafupifupi 100nm.Ebola virus genome ndi RNA yosagawika yosagawika yokhala ndi kukula kwa 18.9kb, ndikuyika mapuloteni 7 opangidwa ndi puloteni imodzi yopanda mawonekedwe.Vuto la Ebola likhoza kugawidwa m'magulu monga Zaire, Sudan, Bundibugyo, Tai Forest ndi Reston.Mwa iwo, mtundu wa Zaire ndi mtundu wa Sudan akuti akupha anthu ambiri chifukwa cha matenda.EHF (Ebola Hemorrhagic Fever) ndi matenda opatsirana omwe amayamba chifukwa cha kachilombo ka Ebola.Anthu amakhudzidwa makamaka ndi kukhudzana ndi madzi a m'thupi, zotuluka ndi zimbudzi za odwala kapena nyama zomwe zili ndi kachilombo, ndipo mawonetseredwe a matendawa amakhala akutuluka malungo, kutuluka magazi komanso kuwonongeka kwa ziwalo zingapo.EHF ili ndi chiwopsezo chachikulu cha kufa kwa 50% -90%.
Channel
| FAM | MP nucleic acid |
| Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Seramu yatsopano, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi / μL |
| Mwatsatanetsatane | Gwiritsani ntchito zida kuti muyese mafotokozedwe olakwika a kampani, zotsatira zimakwaniritsa zofunikira. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, ndi BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Zopangira zopangira zovomerezeka: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) yolembedwa ndi Tiangen Biotech (Beijing) Co.,Ltd.Iyenera kuchotsedwa mosamalitsa malinga ndi malangizo, ndipo voliyumu yotulutsa yovomerezeka ndi 140μL ndipo voliyumu yovomerezeka ndi 60μL.
Njira 2.
Zomwe zimalangizidwa m'zigawo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). ziyenera kuchotsedwa motsatira malangizo.Voliyumu yotulutsa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
Njira 3.
Zopangira zopangira zovomerezeka: Nucleic Acid Extraction Reagent (1000020261) ndi High Throughput Automated Sample Preparation System(MGISP-960) yopangidwa ndi BGI iyenera kuchotsedwa molingana ndi malangizo.Voliyumu yotulutsa ndi 160μL, ndipo voliyumu yovomerezeka ndi 60μL.
