Kachilombo ka malungo a magazi ku Xinjiang
Dzina la chinthu
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka HIV ka Xinjiang hemorrhagic fever kanapezeka koyamba m'magazi a odwala omwe ali ndi matenda a hemorrhagic fever ku Tarim Basin, Xinjiang, China ndi nkhupakupa zolimba zomwe zimapezeka m'deralo, ndipo dzina lake linatchedwa. Zizindikiro zachipatala zimaphatikizapo kutentha thupi, mutu, kutuluka magazi, kuthamanga kwa magazi, ndi zina zotero. Kusintha kwakukulu kwa matendawa ndi kufalikira kwa mitsempha yamagazi, kutsekeka kwa magazi, kuwonjezeka kwa kutseguka kwa magazi ndi kufooka, zomwe zimapangitsa kuti pakhale kutsekeka kwa magazi m'thupi lonse komanso m'maselo a ziwalo zosiyanasiyana, komanso kuwonongeka kwa ziwalo zolimba monga chiwindi, adrenal gland, pituitary gland, ndi zina zotero, komanso kutupa kofanana ndi jelly mu retroperitoneum.
Njira
| FAM | Kachilombo ka malungo a magazi ku Xinjiang |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | seramu watsopano |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | Makopi 1000/mL |
| Kufotokozera Mwapadera | Palibe kuyanjana ndi zitsanzo zina za kupuma monga Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, ndi zina zotero. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500 Real-Time PCR, Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chotsukira chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chotsukira ichi. Kuchuluka kwa chitsanzo chochotsedwa ndi 200µL, ndipo kuchuluka kwa chotsukira komwe kukulangizidwa ndi 80µL.
Chothandizira kuchotsa chomwe chikuyembekezeredwa: QIAamp Viral RNA Mini Kit (52904) ndi QIAGEN ndi Nucleic Acid Extraction kapena Purification Reagent (YDP315-R). Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chochotsedwa ndi 140µL, ndipo kuchuluka kwa elution komwe kukuyembekezeredwa ndi 60µL.
