SARS-CoV-2/chimfine A/chimfine B

SARS-CoV-2/influenza A /influenza B Chithunzi Chowonetsedwa

Kufotokozera Kwachidule:

Chidachi ndi choyenera kuzindikiritsa bwino kwa SARS-CoV-2, fuluwenza A ndi fuluwenza B nucleic acid ya nasopharyngeal swab ndi zitsanzo za oropharyngeal swab zomwe mwa anthu omwe amaganiziridwa kuti ali ndi matenda a SARS-CoV-2, fuluwenza A ndi fuluwenza. B. Itha kugwiritsidwanso ntchito poganiziridwa kuti ndi chibayo komanso milandu yamagulu omwe amaganiziridwa kuti ndi yamagulu komanso kudziwa za SARS-CoV-2, fuluwenza A ndi fuluwenza B nucleic acid mu swab ya nasopharyngeal ndi zitsanzo za oropharyngeal swab za matenda a Coronavirus nthawi zina.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT148-SARS-CoV-2/chimfine A / chimfine B Nucleic Acid Combined Detection Kit (Fluorescence PCR)

Channel

Dzina la Channel	Chithunzi cha PCR-Mix 1	Chithunzi cha PCR-Mix 2
FAM Channel	Mtengo wa ORF1ab	IVA
VIC/HEX Channel	Ulamuliro wamkati	Ulamuliro wamkati
Chithunzi cha CY5	N gene	/
Chithunzi cha ROX	E gene	IVB

Magawo aukadaulo

Kusungirako	-18 ℃
Alumali moyo	12 miyezi
Mtundu wa Chitsanzo	nsonga za nasopharyngeal ndi oropharyngeal swabs
Zolinga	Zolinga zitatu za SARS-CoV-2 (mitundu ya Orf1ab, N ndi E)/chimfine A/chimfine B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2:300 Makopi/mL fuluwenza A virus: 500 Copies/mL fuluwenza B HIV: 500 Makopi / mL
Mwatsatanetsatane	a) Zotsatira zoyeserera zidawonetsa kuti zidazo zinali zogwirizana ndi coronavirus yamunthu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kupuma kwa syncytial virus A ndi B, parainfluenza virus 1, 2 ndi 3, rhinovirus A, B ndi C, adenovirus 1, 2, 3, 4, 5, 7 ndi 55, metapneumovirus yaumunthu, enterovirus A, B, C ndi D, kachilombo ka cytoplasmic pulmonary virus, kachilombo ka EB, kachilombo ka chikuku Human cytomegalovirus, Matenda a rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniaeberculos, Casissiella pneumoniae, Casissiella ta Kunalibe kusiyana pakati pa Pneumocystis yersini ndi Cryptococcus neoformans. b) Kutha kusokoneza: sankhani mucin (60mg/mL), 10% (V/V) magazi amunthu, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (yokhala ndi preservative) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL) ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) ndi tobramycin (0.6mg/mL) .Zotsatirazo zinasonyeza kuti zinthu zosokoneza pazigawo zomwe zili pamwambazi zinalibe zosokoneza zokhudzana ndi zotsatira zowonongeka za tizilombo toyambitsa matenda.
Zida Zogwiritsira Ntchito	Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN^®-96P Real-Time PCR Systems QuantStudio™ 5 Real-Time PCR Systems LightCycler^®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System