SARS-CoV-2 chimfine A fuluwenza B Nucleic Acid Kuphatikiza

Kufotokozera Kwachidule:

Chidachi ndi choyenera kuzindikiritsa bwino kwa SARS-CoV-2, fuluwenza A ndi fuluwenza B nucleic acid ya nasopharyngeal swab ndi zitsanzo za oropharyngeal swab zomwe mwa anthu omwe amaganiziridwa kuti ali ndi matenda a SARS-CoV-2, fuluwenza A ndi fuluwenza. B.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT060A-SARS-CoV-2 chimfine A B Nucleic Acid Combined Detection Kit (Fluorescence PCR)

Satifiketi

AKL/TGA/CE

Epidemiology

Matenda a Corona Virus 2019 (COVID-19) amayamba ndi SARS-CoV-2 yomwe ndi ya β Coronavirus yamtundu.COVID-19 ndi matenda opatsirana owopsa, ndipo unyinji wa anthu nthawi zambiri umakhala wotengeka.Pakadali pano, odwala omwe ali ndi kachilombo ka SARS-CoV-2 ndiye gwero lalikulu la matendawa, ndipo odwala asymptomatic amathanso kukhala gwero la matenda.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1-14, makamaka masiku 3-7.Zisonyezero zazikulu zinali malungo, youma chifuwa ndi kutopa.Odwala ochepa amakhala ndi zizindikiro monga kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba.

Fuluwenza ndi pachimake kupuma matenda chifukwa fuluwenza HIV.Imapatsirana kwambiri ndipo imafalikira makamaka chifukwa cha kutsokomola ndi kuyetsemula.Nthawi zambiri imayamba masika ndi nyengo yozizira.Pali mitundu itatu ya Influenza, Influenza A (IFV A), Influenza B (IFV B) ndi Influenza C (IFV C), onsewa ndi a banja la ortomyxovirus.Fuluwenza A ndi B, omwe ali amodzi-stranded, segmental RNA mavairasi, ndi amene amayambitsa matenda a anthu.Fuluwenza A ndi pachimake kupuma matenda opatsirana, kuphatikizapo H1N1, H3N2 ndi subtypes ena, n'zosavuta kusintha.Kufalikira kwapadziko lonse lapansi, "kusintha" kumatanthauza kusintha kwa chimfine A, zomwe zimapangitsa kuti pakhale kachilombo ka "subtype".Fuluwenza B imagawidwa m'mizere iwiri: Yamagata ndi Victoria.Influenza B imangokhala ndi antigenic drift, ndipo amazemba kuyang'aniridwa ndi kuthetsedwa ndi chitetezo cha mthupi mwa munthu kudzera mukusintha.Koma ma virus a fuluwenza B amasintha pang'onopang'ono kuposa fuluwenza A, yomwe imayambitsanso matenda opumira komanso miliri mwa anthu.

Channel

FAM

SARS-CoV-2

Mtengo ROX

IFV B

CY5

IFV A

VIC (HEX)

Majini olamulira amkati

Magawo aukadaulo

Kusungirako

Zamadzimadzi: ≤-18 ℃ Mumdima

Lyophilization: ≤30 ℃ mumdima

Alumali moyo

Madzi: 9 miyezi

Lyophilization: miyezi 12

Mtundu wa Chitsanzo

Masamba a Nasopharyngeal, Oropharyngeal swabs

Ct

≤38

CV

≤5.0%

LoD

300 Makopi / ml

Mwatsatanetsatane

Zotsatira zoyesa pamtanda zidawonetsa kuti zidazo zinali zogwirizana ndi coronavirus yamunthu SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kupuma kwa syncytial virus A ndi B, parainfluenza virus 1, 2 ndi 3, rhinovirus A, B ndi C, adenovirus 1, 2, 3, 4, 5, 7 ndi 55, metapneumovirus yaumunthu, enterovirus A, B, C ndi D, kachilombo ka cytoplasmic pulmonary virus, kachilombo ka EB, kachilombo ka chikuku Human cytomegalovirus, rotavirus, norovirus, kachilombo ka mumps, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium furunculosis, Caspital pneumoniae, Mycobacterium pneumoniae, Casperococcus pneumoniae mtanda anachita pakati pa Pneumocystis yersini ndi Cryptococcus neoformans.

Zida Zomwe Zingagwiritsidwe Ntchito:

Itha kufanana ndi zida za fulorosenti za PCR pamsika.

Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Kuyenda Ntchito

Njira 1.

Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Njira 2.

Zomwe zimalangizidwa m'zigawo: Nucleic Acid Extraction kapena Purification Reagent(YDP302) yolembedwa ndi Tiangen Biotech(Beijing) Co.,Ltd.


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