Fuluwenza ya SARS-CoV-2 Fuluwenza ya A Fuluwenza ya B Nyukiliya Yophatikizika
Dzina la chinthu
HWTS-RT060A-SARS-CoV-2 fuluwenza A fuluwenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Satifiketi
AKL/TGA/CE
Epidemiology
Matenda a Corona Virus 2019 (COVID-19) amayamba chifukwa cha SARS-CoV-2 yomwe ndi ya β Coronavirus ya mtundu wa β. COVID-19 ndi matenda opatsirana opatsirana m'mapapo, ndipo anthu ambiri amakhala pachiwopsezo. Pakadali pano, odwala omwe ali ndi SARS-CoV-2 ndi omwe amayambitsa matendawa, ndipo odwala omwe alibe zizindikiro amathanso kukhala magwero a matendawa. Kutengera kafukufuku wamakono wa matenda, nthawi yoberekera ndi masiku 1-14, makamaka masiku 3-7. Zizindikiro zazikulu zinali malungo, chifuwa chouma komanso kutopa. Odwala ochepa ali ndi zizindikiro monga kutsekeka kwa mphuno, mphuno yotuluka madzi, pakhosi, myalgia ndi kutsegula m'mimba.
Fuluwenza ndi matenda opatsirana opatsirana omwe amayamba chifukwa cha kachilombo ka fuluwenza. Ndi matenda opatsirana kwambiri ndipo amafalikira makamaka kudzera mu chifuwa ndi kuyetsemula. Nthawi zambiri amafalikira m'nyengo ya masika ndi yozizira. Pali mitundu itatu ya Fuluwenza, Fuluwenza A (IFV A), Fuluwenza B (IFV B) ndi Fuluwenza C (IFV C), onsewa ndi a m'banja la ortomyxovirus. Fuluwenza A ndi B, omwe ndi ma RNA virus omwe ali ndi mzere umodzi, ndi omwe amayambitsa matenda a anthu. Fuluwenza A ndi matenda opatsirana opatsirana opatsirana, kuphatikizapo H1N1, H3N2 ndi mitundu ina, ndi osavuta kusintha. Kufalikira kwa dziko lonse lapansi, "kusintha" kumatanthauza kusintha kwa fuluwenza A, zomwe zimapangitsa kuti pakhale "mtundu wina" watsopano wa kachilomboka. Fuluwenza B imagawidwa m'mizere iwiri: Yamagata ndi Victoria. Fuluwenza B imangokhala ndi ma antigenic drift, ndipo imapewa kuyang'aniridwa ndi chitetezo cha mthupi cha anthu kudzera mu kusintha kwa majini. Koma mavairasi a fuluwenza B amakula pang'onopang'ono kuposa fuluwenza A ya anthu, yomwe imayambitsanso matenda opuma ndi miliri mwa anthu.
Njira
| FAM | SARS-CoV-2 |
| ROX | IFV B |
| CY5 | IFV A |
| VIC(HEX) | Majini a Kulamulira kwa Mkati |
Magawo aukadaulo
| Malo Osungirako | Madzi: ≤-18℃ Mumdima |
| Kupopera kwa mpweya: ≤30℃ mumdima | |
| Nthawi yokhalitsa | Madzi: miyezi 9 |
| Kuchepetsa mpweya m'thupi: miyezi 12 | |
| Mtundu wa Chitsanzo | Ma swab a m'mphuno, ma swab a m'mphuno |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makope 300/mL |
| Kufotokozera Mwapadera | Zotsatira za mayeso osiyanasiyana zasonyeza kuti zidazi zikugwirizana ndi kachilombo ka corona ka anthu SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, kachilombo ka respiratory syncytial virus A ndi B, parainfluenza virus 1, 2 ndi 3, rhinovirusA, B ndi C, adenovirus 1, 2, 3, 4, 5, 7 ndi 55, human metapneumovirus, enterovirus A, B, C ndi D, human cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Panalibe kusiyana pakati pa Pneumocystis yersini ndi Cryptococcus neoformans. |
| Zida Zogwiritsidwa Ntchito: | Ikhoza kufanana ndi zida zazikulu za PCR za fluorescent zomwe zili pamsika. Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Chotsukira chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Njira yachiwiri.
Chothandizira kuchotsa chomwe chikulangizidwa: Nucleic Acid Extraction kapena Purification Reagent (YDP302) ndi Tiangen Biotech (Beijing) Co., Ltd.
