Kodi Ukadaulo Wouma Mozizira Ungapangitse Bwanji Kuzindikira Ma Molekyu Kukhala Kokhazikika, Kotsika Mtengo, Kosavuta, Komanso Kosavuta? Macro & Micro-Test(MMT) Ili ndi Yankho Latsopano!

Popeza kuyezetsa kwa nucleic acid kukukhala kofunikira nthawi zonse, kodi mwakumana ndi mavuto awa: ma reagents omwe amaika pachiwopsezo kuwonongeka panthawi yoyendetsa, njira zotsegulira zomwe zimayambitsa kuipitsidwa, kapena kutayika kwa ntchito chifukwa cha kuzungulira kobwerezabwereza kwa kuzizira ndi kusungunuka?

Ukadaulo wakale wa "zaka zotsutsana"—kuumitsa ndi vacuum—tsopano ikuyendetsa kusintha kwakukulu mu makampani ofufuza za mamolekyu.

Lero, tiyeni tiwone momweMacroMicroTech (MMT)ikulimbana ndi mavuto anayi akuluakulu ndiyogwiritsidwa ntchito kamodzi kokha, yokonzedwa bwino komanso youma mufirijimlengalengaukadaulo.

I. Kubwerera Kodabwitsa kwa Ukadaulo Wakale: Mbiri Yachidule Yowumitsa Madzi Ozizira

Mu 1909, asayansi anayamba kugwiritsa ntchito njira yowumitsa madzi mufiriji kuti asunge seramu; pofika mu 1919, idathandiza kuti mitundu ya mavairasi isungidwe kwa nthawi yayitali. Kale inkadziwika kuti ndi "mtetezi wa zochita zamoyo," njira yowumitsa madzi mufiriji tsopano yabwezeretsedwanso ndipo imagwiritsidwa ntchito kwambiri pofufuza mamolekyu amakono.

1. Ulendo Wovuta wa Ma Reagents Achikhalidwe a Madzi

Kuyambira kwa wopanga mpaka wogwiritsa ntchito, ma reagents amadzimadzi ayenera kusungidwa mosamala kwambiri nthawi yonse yosungidwa ndi kunyamulidwa. Kusagwiritsa ntchito bwino nthawi zambiri kumabweretsa kuwonongeka kwa ma enzyme, kusakhazikika kwa kafukufuku, komanso kulephera kuchitapo kanthu - vuto lomwe limalembedwa mobwerezabwereza m'maphunziro ndi maphunziro a milandu.

2. Moyo wa Zinthu Zoyambitsa Madzi Zoopsa Kwambiri

Bokosi la zinthu zozizira likatsegulidwa, nthawi zambiri limakhala ndi nthawi zambiri zosungunuka, kugwiritsidwa ntchito, ndi kuzizira—mpaka litagwiritsidwa ntchito mokwanira kapena kutayidwa—zomwe zimapangitsa kuti ntchito iwonongeke komanso kutayidwa.

Kuumitsa mufiriji kumathetsa mavutowa pamizu. Mwa kuchotsa madzi pansi pa vacuum pa kutentha kochepa, ma enzyme ndi ma probe "amatsekedwa" mu mkhalidwe wokhazikika - makamaka kukanikiza kuyimitsa pang'onopang'ono pakuwonongeka mpaka nthawi yogwiritsira ntchito.

II. Ubwino Unayi Waukulu: Chifukwa Chake Kuumitsa Mufiriji Ndi Njira Yabwino Kwambiri Yodziwira Matenda a Ma Molekyulu

Ma microspheres a MMT ogwiritsidwa ntchito kamodzi kokha, opangidwa mokwanira kuti achotse zopinga zazikulu zamakampani: 

III. Kupambana kwa Ukadaulo: Chifukwa Chiyani MMT Inasankha Micro Youma Yozizirachozunguliras?

Pakati pa mitundu yosiyanasiyana monga lyophilization ya mkati mwa vial kapena PCR-tube, MMT yakhala ikutsogoleramicro youma mufirijichozunguliramtunduzomwe zimakwaniritsa zinthu zitatu zofunika kwambiri:

iwe"ChimodziMicrosphere= Kuyankha Konse Kokwanira”

Microsphere iliyonse ya 2 mm ili ndi zinthu zonse

—ma primers, ma enzyme, ma dNTPs—kutsimikizira kulondola ndikuchotsa zolakwika pakulemba.

iweKukonzanso kwa Mphindi 1

Zinthu zina zapadera zimathandiza kuti ma microspheres asungunuke mofulumira ndi 50% kuposa mitundu yachizolowezi youma ndi kuzizira.

iweKugwirizana kwa Mawonekedwe Otseguka

Imagwirizana ndi zinthu zosiyanasiyana zogwiritsidwa ntchito ndi zipangizo (monga machubu a PCR a 8-strip, mitundu yogwiritsidwa ntchito kamodzi), kuonetsetsa kuti zinthuzo zikusintha mosavuta pa ntchito zonse.

IV. Tsogolo Lili Tsopano: Momwe Kuumitsa Kozizira Kudzasinthira Kuzindikira Ma Molekyulu

Pamene njira zodziwira matenda a mamolekyu zikuyandikira malo osamalira odwala, kuyezetsa kunyumba, ndi malo oyambira azaumoyo, MMT yakonzeka ndi nsanja yake yolimba yowumitsa.
Mwachitsanzo, poyesa mapanelo opumira, ma microspheres ouma mozizira amapereka zotsatira zodalirika mosavuta, popanda unyolo wozizira kapena kukhazikika kovuta.

Ndondomeko Yachikhalidwe:

Pa AIO800

Kuchokera"Nkhawa ya unyolo wozizira"ku"Kumasuka kutentha kwa chipinda,"kuchokera"Njira zovuta"ku"Kuzindikira kwa sitepe imodzi," Ukadaulo wowumitsa mufiriji ukukonzanso malire a kudalirika pakuwunika mamolekyu.
MMT ipitiliza kupanga zinthu zatsopano panjira yokhazikika, yogwira ntchito bwino, yosavuta, komanso yosavuta—kupatsa mphamvu njira zodziwira matenda molondola kuti zithetse nthawi ndi malo oletsa.