Gulu B Streptococcus nucleic acid kuzindikira zida (Enzymatic Probe Isothermal Amplification)
1.Kuzindikira tanthauzo
Gulu B la streptococcus (GBS) nthawi zambiri limakhala mu nyini ndi matumbo a amayi, zomwe zingayambitse matenda oyambilira (GBS-EOS) mwa makanda obadwa kumene kudzera mwa kufalikira kuchokera kwa mayi kupita kwa mwana, ndipo ndizomwe zimayambitsa chibayo cha akhanda, meningitis, septicemia ndi ngakhale imfa.Mu 2021, a Expert Consensus on Clinical Management of High-Risk Infection with Early-onset Infection in Same Room of Mothers and Infants of China Maternal and Child Health Association inanena kuti GBS iwonetsere masabata 35-37 asanabadwe komanso kupewa intrapartum antibiotic (IAP). ) anali njira zothandiza kuteteza GBS-EOS mwa ana obadwa kumene.
2. Mavuto omwe akukumana nawo ndi njira zamakono zodziwira
Kuphulika msanga kwa nembanemba (PROM) kumatanthauza kung'ambika kwa nembanemba isanabale, zomwe ndizovuta zomwe zimachitika nthawi yobereka.Kuphulika msanga kwa nembanemba Chifukwa cha kusweka kwa nembanemba, GBS mu nyini ya amayi obadwa nthawi zambiri imafalikira mmwamba, zomwe zimapangitsa kuti pakhale matenda a intrauterine.Chiwopsezo chotenga kachilomboka chimayenderana mwachindunji ndi kakulidwe kamene kakulidwe ka nembanemba (> 50% ya amayi apakati amabala mkati mwa maola 1-2 pambuyo pa kuphulika kwa nembanemba, kapena maola 1-2).
Njira zodziwira zomwe zilipo sizingathe kukwaniritsa zofunikira pa nthawi yake (< 1h), kulondola komanso kuzindikira kwa GBS pa foni panthawi yobereka.
| Zida zodziwira | Bakiteriya chikhalidwe | Chikhalidwe nthawi: 18-24hNgati mankhwala tilinazo mayeso : kuwonjezeka 8-16h | 60% yodziwika bwino;poyesa, amatha kutenga mabakiteriya monga Enterococcus faecalis mozungulira nyini ndi anus, zomwe zimapangitsa kuti pakhale zotsatira zabodza / zabodza. |
| Immunochromatography | Nthawi yozindikira: 15min. | Kumverera kumakhala kochepa, ndipo n'kosavuta kuphonya kuzindikira, makamaka pamene kuchuluka kwa mabakiteriya kuli kochepa, n'kovuta kuzindikira, ndipo malangizowo sakulimbikitsidwa. | |
| PCR | Nthawi yozindikira: 2-3h | Nthawi yodziwikiratu ndi yoposa maola a 2, ndipo chida cha PCR chiyenera kuyesedwa m'magulu, ndipo sizingatheke kutsatira mayesero. |
3. Macro & Micro-Test zinthu zazikulu
Kuzindikira mwachangu: Pogwiritsa ntchito njira yoyezera kutentha kwa enzyme yokhazikika, odwala omwe ali ndi chiyembekezo amatha kudziwa zotsatira zake pakangotha mphindi zisanu.
Kuzindikira nthawi iliyonse, palibe chifukwa chodikirira: imakhala ndi kutentha kosalekeza kwa nucleic acid amplification analyzer Easy Amp, ndipo ma modules anayi amayendetsa paokha, ndipo zitsanzo zimayesedwa pamene zikufika, kotero palibe chifukwa chodikirira zitsanzo.
Mitundu yambiri ya zitsanzo: kutsekemera kwa nyini, swab ya rectal kapena swab yosakanikirana ya vaginal imatha kuzindikirika, yomwe imagwirizana ndi malangizo a GBS, imapangitsa kuti anthu azidziwika bwino komanso amachepetsa chiwerengero cha matenda olakwika.
Kuchita bwino kwambiri: kutsimikizira kwachipatala kwamitundu yambiri (> milandu 1000), kumva 100%, kutsimikizika 100%.
Open reagent: yogwirizana ndi chipangizo chaposachedwa cha fluorescence quantitative PCR.
4. Zambiri zamalonda
| Nambala Yogulitsa | Dzina lazogulitsa | Kufotokozera | Nambala ya satifiketi yolembetsa |
| Chithunzi cha HWTS-UR033C | Gulu B Streptococcus nucleic acid kuzindikira zida (Enzymatic Probe Isothermal Amplification) | 50 mayeso / zida | China Machinery Kulembetsa 20243400248 |
| Mbiri ya HWTS-EQ008 | HWTS-1600P (4-channel) | China Machinery Kulembetsa 20233222059 | |
| HWTS-1600S (2-channel) | |||
| Mbiri ya HWTS-EQ009 |
Nthawi yotumiza: Mar-07-2024