EasyAmp by Macro & Micro Test—A Portable Isothermal Fluorescence Amplification Instrument Yogwirizana ndi LAMP/RPA/NASBA/HDA

Kuchita Kwabwino Kwambiri & Ntchito Yonse

Easy Amp, mwaukadaulo wa isothermal nucleic acid amplificationimawonetsedwa ndi mkulusensitivity ndimwachiduleanachitanthawi popanda zofunikira kutentha kusintha ndondomeko. Chifukwa chake, zaterokutulukiraedngatiambiriukadaulo wokondedwa woyesa mwachangu nucleic acid.Popanda zovuta tkachitidwe ka njinga zamtundu wa hermal, chipangizo chosavuta komanso chonyamula cha isothermal amplificationakhoza kukwaniritsalonse reaction process.

Macro & Micro-Test adapanga paokhandipo anayambitsandi EasyAmp,chida chonyamulika cha fulorosenti isothermal amplificationza ntchito zosiyanasiyana.

Pakadali pano, aEasy Amp zatsimikiziridwa kukhalaImagwirizana ndi njira zingapo zokulira za isothermal: Loop-Mediated Isothermal Amplification(LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification(NASBA), ndi Helicase Dependent Amplification(HDA).Chidzakhala chida china chamakono choyesera mwachangu ma nucleic acid ndikugwiritsa ntchito mokulirapo.

Kukweza kwa Isothermal (LAMP) kwa Loop-Mediated:Amplification pa60-65 ° Cndi lonseanachitandondomekoanamaliza mkati mwa mphindi 30.Kuwona Chithunzi 1 pakukulitsa kwakemfundo:

 

Chithunzi 1. Mfundoof LAMP 

Recombinase Polymerase Amplification (RPA/RAA/MIRA):Amplification pa37°C-42°Cndi lonseanachitandondomekoanamaliza mu 10-30 mphindi.Kuwona Chithunzi 2 chakemfundo ya amplification:

Chithunzi 2. Mfundoof Recombinase Polymerase Amplification

·Nucleic Acid Sequence Based Amplification (NASBA):Amplification pa42°Cndi lonseanachitandondomekoanamaliza mu 40-120 mphindi.Kuwona Chithunzi 3 chakemfundo ya amplification:

Chithunzi 3. Mfundoof Nucleic Acid Sequence Based Amplification

· Helicase Dependent Amplification (HDA):Amplification pa65°Cndi lonseanachitandondomekoanamaliza mu 70-90 mphindi.Kuwona Chithunzi 4 chakemfundo ya amplification:

Chithunzi 4.Mfundo yofunikaof Helicase Dependent Amplification

· EasyAmpathekukulitsa kogwira mtima komanso kokhazikika pakuzindikirika kwa DNA ndi RNA pogwiritsa ntchito njira zinayi zomwe zatchulidwazi.Imakwaniritsa kukhudzika kwamphamvu mpaka makope a 200/mL ndikuwonetsetsa kutsimikizika kwakukulu popanda kubwerezanso pakukulitsa chandamale..(Ikufotokozedwa mu Zithunzi 5 mpaka 7).

Kuzindikira kwa DNA RNA

Chithunzi 5.Pulogalamu ya RAA/MIRA

Kuzindikira kwa DNA RNA

Chithunzi 6.Pulogalamu ya LAMP

 

Kuzindikira kwa DNA RNA

Chithunzi 7.NASBA Platform

Ubwino wazinthu:

Mwamsanga

· EPIluso amazindikirazotsatira zabwinozadziwika mu 5 min.

· Ntchito yosavuta yokhala ndi gawo limodzikwa amplication pa chida.

Kuchita bwino

· Ma module 4 odziyimira pawokha pakufunidwa, kuyesa nthawi imodzi kwa 4 osiyanasiyanakuyesa mtundus.

· Mutu uliwonse wokhoza kusanthula zitsanzo zinayi nthawi imodzi.

· Awiri / Quadfluorescencenjirakuti mudziwe zambiri za zolinga zambiri.

Kugwirizana

· Yogwirizana ndizambirinsanja za isothermal monga LAMP, RPA, NASBA, HDA.

· Zapangidwira kuti zisindikizidwe zokulitsa kutentha kwambiri pambuyo pa kutentha pang'ono.

· Customizwokhozakukwaniritsa zosowa za kasitomala.

Yosavuta kugwiritsa ntchito

· Portable kapangidwe(290×245×128 mm, 2kg) pa zosiyanasiyana ntchito masenario.

· 7-inch color touchscreenkuti mugwiritse ntchito mosavuta komanso fanizo lomveka bwino la zotsatira.

· Makina osindikizira otenthetsera kuti asindikize posachedwa.

· Chojambulira chophatikizika chowerengera zinthu zodziwikiratu.