Mayeso a EasyAmp ndi Macro & Micro—-Chida Chonyamulika Chowonjezera Mphamvu Yotentha Yochokera ku Isothermal Chogwirizana ndi LAMP/RPA/NASBA/HDA

Magwiridwe Abwino Kwambiri & Kugwiritsa Ntchito Konse

Easy Amp, pogwiritsa ntchito ukadaulo wa ikukulitsa kwa nucleic acid ya sothermalimawonetsedwa ndi mkulukukhudzidwa ndimwachidulekuchitapo kanthunthawi yopanda zofunikira za njira yosinthira kutentha. Chifukwa chake, ili ndikutulukaedmongaambiriukadaulo wokonda kwambiri woyesera mwachangu nucleic acid.Popanda zovutamakina oyendera njinga za zomera, chipangizo chosavuta komanso chonyamulika chowonjezera kutentha kwa thupiakhoza kukwaniritsanjira yonse yochitira zinthu.

Macro & Micro-Test yapangidwa payokhandipo idayambitsidwaEasyAmp,chida chonyamulika cha fluorescent isothermal amplificationpa ntchito zosiyanasiyana.

Pakadali pano,Amp Yosavuta yatsimikiziridwa kuti ndiYogwirizana ndi njira zingapo zokwezera kutentha: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), ndi Helicase Dependent Amplification (HDA)Idzakhala chida china chamakono choyesera mwachangu nucleic acid chomwe chingagwiritsidwe ntchito kwambiri.

· Kukulitsa kwa Isothermal Kozungulira (NYALE):Kukulitsa pa60-65°Cndi zonsekuchitapo kanthunjirazatha mkati mwa mphindi 30.Kuwona Chithunzi 1 cha kukulitsa kwakemfundo:

 

Chithunzi 1. Mfundoof NYALI 

· Kukulitsa kwa Recombinase Polymerase (RPA/RAA/MIRA):Kukulitsa pa37°C-42°Cndi zonsekuchitapo kanthunjirazatha mu mphindi 10-30.Kuwona Chithunzi 2 chamfundo yowonjezerera:

Chithunzi 2. Mfundoof Kukulitsa kwa Recombinase Polymerase

·Kukulitsa kwa Nucleic Acid Sequence Based Amplification (NASBA):Kukulitsa pa42°Cndi zonsekuchitapo kanthunjirazatha mu mphindi 40-120.Kuwona Chithunzi 3 chamfundo yowonjezerera:

Chithunzi 3. Mfundoof Kukulitsa Kochokera ku Nucleic Acid Sequence

· Kukulitsa Kodalira Helicase (HDA):Kukulitsa pa65°Cndi zonsekuchitapo kanthunjirazatha mu mphindi 70-90.Kuwona Chithunzi 4 chamfundo yowonjezerera:

Chithunzi 4. Mfundoof Kukulitsa Kodalira Helicase

· EasyAmpthandizaniKukulitsa kwabwino komanso kokhazikika kwa DNA ndi RNA pogwiritsa ntchito njira zinayi za isothermal zomwe zatchulidwazi. Kumakwaniritsa kukhudzika kwa kukulitsa mpaka makope 200/mL ndipo kumatsimikizira kutsimikizika kwakukulu popanda kusinthasintha pakukulitsa cholinga..(Izomwe zafotokozedwa mu Zithunzi 5 mpaka 7).

Kuzindikira DNA Kuzindikira RNA

Chithunzi 5. Nsanja ya RAA/MIRA

Kuzindikira DNA Kuzindikira RNA

Chithunzi 6. Pulatifomu ya Nyali

 

Kuzindikira DNA Kuzindikira RNA

Chithunzi 7. Nsanja ya NASBA

Ubwino wa Zamalonda:

Mwachangu

· EPIAukadaulo umakwaniritsazotsatira zabwinozapezeka mu mphindi 5.

· Ntchito yosavuta ndi kuchotsa sitepe imodzizokulitsa chida.

Wogwira ntchito bwino

· Ma module 4 odziyimira pawokha oyesera nthawi imodzi a mitundu 4 yosiyanasiyana pakafunikamtundu woyeseras.

· Gawo lililonse lotha kusanthula zitsanzo zinayi nthawi imodzi.

· Wachiwiri/Wachinayifluorescencenjirakuti mupeze zambiri zokhudza zinthu zambiri.

Kugwirizana

· Yogwirizana ndizingaponsanja za isothermal monga LAMP, RPA, NASBA, HDA.

· Yopangidwira kukulitsa kutentha kwambiri pambuyo pa kutentha kochepa.

· Sinthani Mwamakondawokhozakukwaniritsa zosowa za makasitomala enieni.

Yosavuta kugwiritsa ntchito

· Pkapangidwe ka tebulo lokongola(290×245×128mm, 2 kg) kwa mitundu yosiyanasiyana ya ma senarios ogwiritsira ntchito.

· Chojambula chamtundu wa mainchesi 7kuti ntchito ikhale yosavuta komanso kuti iwonetse zotsatira zake momveka bwino.

· Chosindikizira cha kutentha chomangidwa mkati kuti chisindikizidwe nthawi yomweyo.

· Chojambulira cholumikizidwa kuti chizindikirike chokha.