Nkhani - Comprehensive Mpox Detection Kits (RDTs, NAATs and Sequencing)

Kuyambira Meyi 2022, milandu ya mpox yakhala ikunenedwa m'maiko ambiri omwe siafala padziko lonse lapansi omwe amafalitsa anthu ammudzi.

Pa Ogasiti 26, World Health Organisation (WHO) idakhazikitsa dziko lonse lapansiKukonzekera Kwadongosolo ndi Mayankhokuletsa kufalikira kwa mpox kuchokera kwa munthu kupita kwa munthu kudzera m'mayesero ogwirizana padziko lonse lapansi, m'chigawo, ndi m'dziko. Izi zikutsatira chilengezo chavuto lazaumoyo wa anthu padziko lonse lapansi ndi Director-General wa WHO pa 14 Ogasiti.

Zindikirani kuti kuphulika kwa mpox nthawi ino ndi yosiyana ndi yomwe inachitika mu 2022, yomwe inkafala kwambiri pakati pa amuna omwe amagonana ndi amuna, ndipo chiwerengero cha imfa za anthu omwe ali ndi kachilomboka chinali chosakwana 1%.

Mtundu waposachedwa wa "Clade Ib", womwe ndi wosiyana wa Clade I, uli ndi chiwopsezo chachikulu cha kufa. Kusintha kwatsopano kumeneku kunayamba kufalikira ku DRC mwezi wa September watha, poyamba pakati pa anthu ochita zachiwerewere, ndipo tsopano zafalikira kumagulu ena, ndi ana omwe ali pachiopsezo.

Bungwe la Africa CDC linanena mu lipoti mwezi watha kuti miliri ya mpox yapezeka m'mayiko 10 a mu Africa chaka chino, kuphatikizapo DRC, yomwe yanena kuti 96.3% ya milandu yonse mu Africa chaka chino ndi 97% ya imfa. Ndizofunikira kudziwa kuti pafupifupi 70% ya milandu ku DRC ndi ana azaka zapakati pa 15, ndipo gulu ili ndi 85% yaimfa mdziko muno.

Mpox ndi matenda a zoonosis omwe amayamba chifukwa cha kachilombo ka mpox ndi nthawi yoyambira masiku 5 mpaka 21, makamaka masiku 6 mpaka 13. Munthu amene ali ndi kachilomboka amakhala ndi zizindikiro monga kutentha thupi, kupweteka kwa mutu ndi kutupa kwa lymph nodes, kenako zidzolo kumaso ndi mbali zina za thupi, zomwe zimayamba pang’onopang’ono n’kukhala ma pustules ndipo zimatha kwa pafupifupi mlungu umodzi musanabadwe. Mlanduwu umapatsirana kuyambira pomwe zizindikiro zimayamba mpaka zipsera zimagwera mwachibadwa.

Macro & Micro-Test ikupereka mayeso ofulumira, zida zama cell ndi njira zotsatirira kuti azindikire kachilombo ka mpox, kuthandizira kuzindikira kwa kachilombo ka mpox munthawi yake, kuyang'anira komwe adachokera, mzere, kufalikira ndi kusiyanasiyana kwa ma genomic:

Antigen ya Monkeypox VirusZida Zozindikira (Immunochromatography)

Zitsanzo zosavuta (zotupa zamadzimadzi / pakhosi) ndi zotsatira zachangu mkati mwa 10-15 min;

Kukhudzika kwakukulu ndi LoD ya 20pg/mL yophimba Clade I & II;

Kukhazikika kwapamwamba kopanda kuyanjananso ndi kachilombo ka nthomba, varicella zoster virus, rubella virus, herpes simplex virus, etc.

OPA ya 96.4% poyerekeza ndi NAATs;

Ntchito zambiri monga miyambo, ma CDC, malo ogulitsa mankhwala, zipatala, zipatala kapena kunyumba.

Monkeypox-virus IgM/IgG Antibody Detection Kit(Immunochromatographhy)

Kuchita kosavuta kopanda zida komanso zotsatira zachangu mkati mwa mphindi 10;

Kukhudzika kwakukulu komanso kutsimikizika kwa Clade I & II;

Amazindikira IgM ndi IgG kuti asankhe magawo a matenda a mpox;

Lonse ntchito monga miyambo, CDCs, pharmacies, zipatala, zipatala kapena kunyumba;

Zoyenera pakuwunika kwakukulu kwa matenda omwe akuganiziridwa kuti ali ndi mpox.

Monkeypox Virus Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Kukhudzika kwakukulu ndi LoD ya 200 Copies/mL yokhala ndi IC, yofanana ndi florescence PCR;

Kugwira ntchito kosavuta: Zitsanzo za Lysed zowonjezeredwa ku chubu cha lyophilized reagent kuti chiwonjezeke pakufuna kwachindunji chothandizidwa ndi ma module odziimira a Easy Amp System;

Mkulu mwapadera popanda reactivity mtanda ndi kachilombo ka nthomba, katemera kachilombo, cowpox HIV, mbewa kachilombo, nsungu simplex HIV, varicella-zoster kachilombo, ndi matupi athu anthu, etc.;

Zitsanzo zosavuta (rash fluid / oropharyngeal swab) ndi zotsatira zabwino kwambiri mkati mwa 5 min;

Kuchita bwino kwachipatala komwe kumakhudza Clade I & II yokhala ndi PPA ya 100%, NPA ya 100%, OPA ya 100% ndi Kappa Value ya 1.000 poyerekeza ndi zida za Fluorescence PCR;

Mtundu wa Lyophilized womwe umafuna zoyendera kutentha ndi kusungirako zimathandizira kupezeka m'magawo onse;

Zochitika zosinthika m'zipatala, malo osamalira chipatala, pamodzi ndi Easy Amp kuti muzindikire zomwe mukufuna;

Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Jini yapawiri yolunjika yokhala ndi chidwi chachikulu ndi LoD ya makope 200/mL;

kusinthasintha zitsanzo za zidzolo madzimadzi, swab pakhosi ndi seramu;

Mkulu mwapadera popanda reactivity mtanda ndi kachilombo ka nthomba, katemera kachilombo, cowpox HIV, mbewa kachilombo, nsungu simplex HIV, varicella-zoster kachilombo, ndi matupi athu anthu, etc.;

Kugwira ntchito kosavuta: lysis yofulumira yachitsanzo ndi reagent yotulutsidwa kuti ionjezedwe ku chubu chomwe;

Kuzindikira mwachangu: zotsatira mkati mwa 40 min;

Kulondola kumatsimikiziridwa ndi kuwongolera kwamkati kuyang'anira njira yonse yodziwira;

Kuchita bwino kwachipatala komwe kumakhudza Clade I & II yokhala ndi PPA ya 100%, NPA ya 99.40%, OPA ya 99.64% ndi Kappa Value ya 0.9923 poyerekeza ndi kutsatizana;

Mtundu wa Lyophilized womwe umafuna zoyendera kutentha ndi kusungirako zimathandizira kupezeka m'magawo onse;

Yogwirizana ndi Fluorescence PCR Systems;

Zochitika zosinthika za zipatala, ma CDC ndi ma lab;

Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Kuphimba kwathunthu: kuyesa mavairasi onse a 4 orthopox omwe amatha kupatsira anthu ndi mpox yofala (Clade I & II ikuphatikizidwa) muyeso limodzi kuti asapezeke;

Kukhudzika kwakukulu ndi LoD ya 200 makope / mL;

Mkulu mwapadera popanda reactivity mtanda ndi tizilombo toyambitsa matenda kumayambitsa totupa monga herpes simplex kachilombo, varicella-zoster virus, ndi matupi athu a anthu, etc.;

Ntchito yosavuta: lysis yofulumira yachitsanzo ndi reagent yotulutsidwa kuti ionjezedwe ku buffer imodzi yamachubu;

Kuzindikira mwachangu: kukulitsa mwachangu ndi zotsatira mkati mwa 40 min;

Kulondola kumatsimikiziridwa ndi kuwongolera kwamkati kuyang'anira njira yonse yodziwira;

Yogwirizana ndi Fluorescence PCR Systems;

Zochitika zosinthika za zipatala, ma CDC ndi ma lab;

MonkeypoxVirisi TypingNucleicAcidDetectionKizi (Fluorescence PCR)

Panthawi imodzimodziyo imazindikiritsa Clade I ndi Clade II, yomwe ndiyofunikira pakumvetsetsa momwe kachilomboka kamayambitsa matenda, kutsata momwe kachilomboka kamafalikira, ndikupanga njira zopewera komanso zowongolera.

Kukhudzika kwakukulu ndi LoD ya makope 200/mL;

kusinthasintha zitsanzo za zidzolo madzimadzi, oropharyngeal swab ndi seramu;

Kukhazikika kwapamwamba popanda reactivity pakati pa Clade I ndi II, tizilombo toyambitsa matenda toyambitsa totupa monga herpes simplex virus, varicella-zoster virus, and human genome, etc.;

Ntchito yosavuta: lysis yofulumira yachitsanzo ndi reagent yotulutsidwa kuti ionjezedwe ku buffer imodzi yamachubu;

Kuzindikira mwachangu: zotsatira mkati mwa 40 min;

Kulondola kumatsimikiziridwa ndi kuwongolera kwamkati kuyang'anira njira yonse yodziwira;

Mtundu wa Lyophilized womwe umafuna zoyendera kutentha ndi kusungirako zimathandizira kupezeka m'magawo onse;

Yogwirizana ndi Fluorescence PCR Systems;

Zochitika zosinthika za zipatala, ma CDC ndi ma lab;

Monkey Virus Universal Genome YonseKuzindikiraZida (Multi-PCR NGS)

Monkeypox Virus Whole Genome Detection Kit Yopangidwa kumene ndi Macro & Micro-Test pazosintha zosiyanasiyana, kuphatikiza ndi sequencer ya ONT nanopore, imatha kupeza MPXV yonse ya genome yokhala ndi kuphimba kosachepera 98% mkati mwa maola 8.

Zosavuta kugwiritsa ntchito: luso lokhala ndi chilolezo cha sitepe imodzi yokulitsa, mndandanda wonse wa majeremusi a mpox virus ukhoza kupezedwa ndi kukulitsa kozungulira;

Zomverera komanso zolondola: zimazindikira zitsanzo zotsika mpaka 32CT, ndipo 600bp amplicon nanopore sequencing imatha kukumana ndi msonkhano wapamwamba kwambiri wa genome;

Kuthamanga kwambiri: ONT imatha kumaliza kusonkhanitsa ma genome mkati mwa maola 6-8;

Kugwirizana kwakukulu: ndi ONT, Qi Carbon, SALUS Pro, lllumina, MGI ndi zina zazikulu 2^ndndi 3^rdzotsatizana za m'badwo.

Zovuta kwambiriMonkey Virus Whole GenomeKuzindikiraZida- Illumina/MGI(Multi-PCR NGS)

Kutengera kuchuluka kwa zomwe zilipo 2^ndma sequencers amtundu padziko lonse lapansi, Macro & Micro-Test yapanganso zida zodziwikiratu kuti zigwirizane ndi ma sequencers odziwika bwino kuti akwaniritse kutsatizana kocheperako kwa ma virus ma genome;

Kukulitsa koyenera: 1448 mapeyala a 200bp amplicon ultra-dense primer design kuti azitha kukulitsa bwino komanso kuphimba yunifolomu;

Kugwira ntchito kosavuta: Laibulale ya Mpox virus llumina/MGI ikhoza kupezedwa kudzera pakukulitsa maulendo awiri mu maola a 4, kupewa njira zomangira laibulale zovuta komanso ndalama za reagent;

Kukhudzika kwakukulu: kumazindikira zitsanzo zotsika mpaka 35CT, kupewa bwino zotsatira zabodza zomwe zimayambitsidwa ndi kuwonongeka kwa zidutswa kapena nambala yotsika;

Kulumikizana kwakukulu ndi mainstream 2^ndotsata mibadwo monga lllumina, Salus Pro kapena MGI;Mpaka pano, milandu yopitilira 400 yachipatala yamalizidwa.

Nthawi yotumiza: Aug-28-2024

Comprehensive Mpox Detection Kits (RDTs, NAATs ndi Sequencing)