Mycoplasma hominis, Ureaplasma urealyticum ndi Gardnerella vaginalis Nucleic Acid
Dzina la chinthu
HWTS-UR044-Mycoplasma hominis, Ureaplasma urealyticum ndi Gardnerella vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Mycoplasma hominis (MH) ndi mtundu wa mycoplasma womwe umapezeka m'njira ya mkodzo ndi ziwalo zoberekera ndipo ungayambitse matenda a mkodzo ndi kutupa kwa ziwalo zoberekera. Mycoplasma hominis imapezeka kwambiri mwachilengedwe ndipo imagwirizanitsidwa ndi matenda osiyanasiyana a m'mimba monga nongonococcal urethritis, female cervicitis, adnexitis, infertility, etc. Ureaplasma urealyticum (UU) ndi kachilombo kakang'ono kwambiri ka prokaryotic celled microorganism komwe kamakhala pakati pa mabakiteriya ndi mavairasi ndipo kamakhala paokha, komanso ndi kachilombo kakang'ono kamene kamayambitsa matenda oberekera ndi ziwalo zoberekera mosavuta. Kwa amuna, kangayambitse prostatitis, urethritis, pyelonephritis, ndi zina zotero; kwa akazi, kamayambitsa kutupa m'njira yoberekera monga vaginitis, cervicitis, ndi matenda otupa m'chiuno, ndipo ndi imodzi mwa matenda omwe amayambitsa kusabereka ndi kupititsa padera. Choyambitsa vaginitis chofala kwambiri mwa akazi ndi bacterial vaginosis, ndipo mabakiteriya ofunikira kwambiri a bacterial vaginosis ndi Gardnerella vaginalis. Gardnerella vaginalis (GV) ndi kachilombo koyambitsa matenda komwe sikumayambitsa matenda kakakhala kochepa. Komabe, pamene mabakiteriya akuluakulu a m'mimba a Lactobacilli achepa kapena kuchotsedwa, zomwe zimapangitsa kuti pakhale kusalingana m'malo obisika a m'mimba, Gardnerella vaginalis imachulukana kwambiri, zomwe zimapangitsa kuti pakhale bakiteriya vaginosis. Nthawi yomweyo, tizilombo tina (monga Candida, Neisseria gonorrhoeae, Mycoplasma hominis, ndi zina zotero) timakhala ndi mwayi wolowa m'thupi la munthu, zomwe zimayambitsa vaginitis yosakanikirana ndi cervicitis. Ngati vaginitis ndi cervicitis sizipezeka ndi kuchiritsidwa mwachangu komanso moyenera, pakhoza kukhala matenda opatsirana kudzera m'matenda obereketsa omwe amafalikira m'mbali mwa mucosa ya m'mimba, zomwe zimapangitsa kuti pakhale matenda opatsirana m'mimba monga endometritis, salpingitis, tubo-ovarian abscess (TOA), ndi pelvic peritonitis, zomwe zingayambitse mavuto aakulu monga kusabereka, mimba yotuluka m'mimba komanso zotsatira zoyipa za mimba.
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | swab ya mkodzo ya amuna, swab ya mkodzo ya akazi, swab ya akazi yochokera ku urethra |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | UU, GV 400Kopi/mL; MH 1000Kopi/mL |
| Zida Zogwiritsidwa Ntchito | Ingagwiritsidwe ntchito pa reagent yozindikira mtundu woyamba: Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500 Real-Time PCR, QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni, Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.), Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time (FQD-96A, ukadaulo wa Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Dongosolo la BioRad CFX96 Real-Time PCR, BioRad CFX Opus 96 Real-Time PCR System. Yogwiritsidwa ntchito pa reagent yozindikira mtundu wachiwiri: EudemonTMAIO800 (HWTS-EQ007) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kuyenda kwa Ntchito
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ndi Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (yomwe ingagwiritsidwe ntchito ndi EudemonTM AIO800 (HWTS-EQ007)) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL ndipo kuchuluka kwa elution komwe kumalimbikitsidwa ndi 150μL.
