Mycobacterium Tuberculosis Nucleic Acid ndi Rifampicin (RIF)，Resistance (INH)

Kufotokozera Kwachidule:

Kiti iyi imagwiritsidwa ntchito pozindikira DNA ya Mycobacterium tuberculosis mu sputum ya anthu, solid culture (LJ Medium) ndi liquid culture (MGIT Medium), bronchial lavage fluid, ndi kusintha kwa majini mu 507-533 amino acid codon region (81bp, rifampicin resistance determining region) ya rpoB gene ya Mycobacterium tuberculosis rifampicin resistance, komanso kusintha kwa majini m'malo akuluakulu a Mycobacterium tuberculosis isoniazid resistance. Imathandiza kuzindikira matenda a Mycobacterium tuberculosis, ndipo imazindikira majini akuluakulu a rifampicin ndi isoniazid resistance, zomwe zimathandiza kumvetsetsa kukana mankhwala kwa Mycobacterium tuberculosis yomwe yagwidwa ndi wodwalayo.

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Dzina la chinthu

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid ndi Rifampicin(RIF), (INH) Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, yomwe imatchedwa Tubercle bacillus (TB), ndi mabakiteriya omwe amayambitsa chifuwa chachikulu, ndipo pakadali pano, mankhwala oyamba oletsa chifuwa chachikulu omwe amagwiritsidwa ntchito kwambiri ndi isoniazid, rifampicin ndi ethambutol, ndi zina zotero.^[1]Komabe, chifukwa cha kugwiritsa ntchito molakwika mankhwala oletsa chifuwa chachikulu komanso mawonekedwe a khoma la maselo a mycobacterium TB yokha, mycobacterium TB yakhala ndi kukana mankhwala oletsa chifuwa chachikulu, ndipo mtundu woopsa kwambiri ndi multidrug-resistant tuberculosis (MDR-TB), womwe sulimbana ndi mankhwala awiri odziwika bwino komanso ogwira ntchito, rifampicin ndi isoniazid.^[2].

Vuto la kusamva mankhwala a chifuwa chachikulu likupezeka m'maiko onse omwe bungwe la WHO linafufuza. Kuti pakhale njira zolondola zochiritsira odwala chifuwa chachikulu, ndikofunikira kuzindikira kusamva mankhwala a chifuwa chachikulu, makamaka kusamva kwa rifampicin, komwe kwakhala njira yodziwira matenda yomwe bungwe la WHO limalimbikitsa pochiza chifuwa chachikulu.^[3]Ngakhale kuti kupezeka kwa rifampicin resistance kuli pafupifupi kofanana ndi kupezeka kwa MDR-TB, kungopeza rifampicin resistance kokha kumanyalanyaza odwala omwe ali ndi mono-resistant INH (kutanthauza kukana kwa isoniazid koma okhudzidwa ndi rifampicin) ndi mono-resistant rifampicin (kukhudzidwa ndi isoniazid koma okhudzidwa ndi rifampicin), zomwe zingapangitse odwala kuti alandire chithandizo choyambirira chosayenera. Chifukwa chake, mayeso a isoniazid ndi rifampicin resistance ndi zofunikira zochepa mu mapulogalamu onse oletsa DR-TB.^[⁴^].

Magawo aukadaulo

Malo Osungirako	≤-18℃
Nthawi yokhalitsa	Miyezi 12
Mtundu wa Chitsanzo	Chitsanzo cha Mafinya, Chikhalidwe Cholimba (LJ Medium), Chikhalidwe Chamadzimadzi (MGIT Medium)
CV	<5.0%
LoD	LoD ya zida zodziwira chifuwa chachikulu cha Mycobacterium ndi mabakiteriya 10/mL;LoD ya zida zodziwira mtundu wa rifampicin ndi mtundu wa mutant ndi mabakiteriya 150/mL; LoD ya kit yodziwira mtundu wachilengedwe wa isoniazid ndi mtundu wa mutant ndi mabakiteriya 200/mL.
Kufotokozera Mwapadera	1) Palibe njira yolumikizirana yomwe imachitika mukamagwiritsa ntchito chidachi kuti mupeze DNA ya majini ya munthu (500ng), mitundu ina 28 ya tizilombo toyambitsa matenda tomwe timapuma, ndi mitundu 29 ya mabakiteriya osayambitsa chifuwa chachikulu (monga momwe tawonetsera pa Table 3).2) Palibe kusinthana kwa majini pogwiritsa ntchito chida ichi kuti mudziwe malo omwe majini ena a rifampicin ndi Mycobacterium tuberculosis sagonjetsedwa ndi mankhwala (monga momwe tawonetsera mu Table 4).3) Zinthu zomwe zimasokoneza zomwe zimapezeka m'masampulo oti ayesedwe, monga rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L) mankhwala, sizikhudza zotsatira za mayeso a kit.
Zida Zogwiritsidwa Ntchito	Makina a PCR a SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.), Dongosolo la PCR la BioRad CFX96 Real-Time