Mycoabacterium chifuwa chachikulu nuclec acid ndi rifampicin (rif), kukana (ih)

Kufotokozera kwaifupi:

Kityi imagwiritsidwa ntchito pozindikira ku Vitro Centerterium a Mycobaclisis DNA mwa mtundu wa anthu sputum, mawonekedwe a LJ , rifampicin kukana dedera ya RPOB Gini of Mycobacterium chifuwa chachikulu Rifampicin kukana, komanso kusintha kwa masinthidwe akuluakulu a Mycobactericis a Isoniazid a matenda a My Maniazid. Mycobacterium chifuwa chachikulu chomwe wodwalayo ali nacho.

Tumizani imelo kwa ife

Tsatanetsatane wazogulitsa

Matamba a malonda

Dzina lazogulitsa

HWTS-RT147 Mycobacterium Tuberculosiss nucleclic acid ndi rifampicin (rif), (ih) yopendekera)

Epidemiology

Mycoabacterium chifuwa chachikulu, posachedwapa, bacterium ya Tibercle yomwe imayambitsa chifuwa chachikulu, ndipo pakadali pano, ogwiritsa ntchito mankhwala oyamba ndi chifuwa chachikulu^[1]. Komabe, chifukwa chogwiritsa ntchito mankhwala olakwika a anti-chifuwa chachikulu ndi mawonekedwe a khoma la cenebacteria chifuwa chopangidwa ndi mankhwala osokoneza bongo, ndipo mawonekedwe owopsa ndi chifuwa chachikulu (MDR- TB), yomwe imalimbana ndi mankhwala awiri ofala kwambiri, a Rifampicin ndi eyazid^{[Chithunzi patsamba 2]}.

Vuto la matenda ogwiritsa ntchito mankhwala osokoneza bongo omwe ali m'maiko onse omwe amafunsidwa ndi omwe. Pofuna kupereka zosintha zolondola za odwala matenda a chifuwa chachikulu, ndikofunikira kudziwa kukana mankhwala otsutsa, makamaka rifampicin, yomwe yakhala njira yodziwiratu kuti omwe amachizira chifuwa chachikulu^{[Chithunzi patsamba 3]}. Ngakhale kupezeka kwa Rifampicin kukana kuli kofanana ndi kupezeka kwa MDR-TB kokha kunyalanyaza isoniazic) ndi rifampicin (chidwi cha iyoniazid koma kukana Rifampicin), yomwe ingayambitse odwala omwe ali ndi malingaliro osafunikira Chithandizo cha mankhwala. Chifukwa chake, kuyeserera kwa Rifampicin ndi Rifampicin ndi zofunikira zochepa pazofunikira zonse za Dr-TB Control^[⁴^].

Magawo aluso

Kusunga	≤-18 ℃
Alumali-Moyo	Miyezi 12
Mtundu wofanana	Sputum Sputum, chikhalidwe cholimba (lj sing'anga), chikhalidwe chamadzimadzi (mgit sing'anga)
CV	<5.0%
Lodu	Lod wa Kit kuti adziwe chifuwa cha Mycobacterium ndi 10 bacteria / ml;Lod wa Kit kuti adziwe mtundu wa Rifampicin wamtchire komanso mtundu wa Mutant ndi mabakiteriterite / ml; Lod wa Kit kuti adziwe mtundu wamtchire wamtchire ndi mtundu wa khola ndi mabakiteriya / ml.
Chifanizo	1) Palibe mtanda mukamagwiritsa ntchito zida za mtundu wa anthu (500ng), mitundu ina 28 ya mizere ya kupuma, ndi mitundu 29 ya Mycobacteria (monga akuwonetsera mu tebulo 3).2) Palibe mtanda mukamagwiritsa ntchito zida zosinthanirana ndi mitundu ina ya mankhwala a rifampicin ndi isoniazid sobercrial chifuwa chachikulu (monga tawonera pagome 4).3) Zofananira zomwe zimayesedwa, monga rifampicin (9mg / l), eyazi / l), a oxymetrazoline (1mg / l), Mupirocin (20mg / l), pyrazinamide (45mg / l), Zanjamivir (0.5mg / l), dexamethasone (20mg / l) mankhwala, alibe zotsatira za mayeso a zida.
Zida Zogwirizira	Slan-96P yeniyeni PCR Systems (Hongshi Chaumil Technology Co., Ltd.), Biorad Cfx96 PCR PCR System