Mycobacterium Tuberculosis Nucleic Acid ndi Rifampicin(RIF),Kukaniza(INH)

Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Resistance(INH) Chithunzi Chowonetsedwa

Kufotokozera Kwachidule:

Chidachi chimagwiritsidwa ntchito pozindikira mu vitro qualitative ya Mycobacterium tuberculosis DNA mu sputum ya anthu, chikhalidwe cholimba (LJ Medium) ndi chikhalidwe chamadzimadzi (MGIT Medium), bronchial lavage fluid, ndi masinthidwe a 507-533 amino acid codon region (81bp, rifampicin resistance determining region of mycompicium tubercocci) komanso kusintha kwa masinthidwe a malo akuluakulu a Mycobacterium tuberculosis isoniazid resistance.Imathandiza kuzindikira matenda a chifuwa chachikulu cha Mycobacterium, ndipo imazindikira majini akuluakulu a rifampicin ndi isoniazid, omwe amathandiza kumvetsetsa kukana kwa mankhwala kwa Mycobacterium tuberculosis yomwe wodwala ali nayo.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), (INH) Detection Kit (Melting Curve)

Epidemiology

Mycobacterium tuberculosis, yomwe yangotsala pang'ono kutchedwa Tubercle bacillus (TB), ndi tizilombo toyambitsa matenda amene amayambitsa chifuwa chachikulu cha TB, ndipo pakali pano, mankhwala othana ndi chifuwa chachikulu omwe amagwiritsidwa ntchito nthawi zambiri amaphatikizapo isoniazid, rifampicin ndi ethambutol, ndi zina zotero.^[1]. Komabe, chifukwa cha kugwiritsidwa ntchito molakwika kwa mankhwala odana ndi TB ndi makhalidwe a cell khoma dongosolo mycobacterium TB palokha, mycobacterium TB wapanga mankhwala kukana mankhwala odana ndi TB, ndipo mawonekedwe owopsa kwambiri ndi multidrug zosagwira chifuwa chachikulu (MDR-TB), amene kugonjetsedwa ndi mankhwala awiri ambiri komanso ogwira ntchito, rifampicin ndi rifampicin.^[2].

Vuto la chifuwa chachikulu chosamva mankhwala lilipo m’maiko onse amene bungwe la WHO linafufuza. Pofuna kupereka ndondomeko zolondola za chithandizo cha odwala chifuwa chachikulu, m'pofunika kudziwa kukana mankhwala oletsa chifuwa chachikulu, makamaka rifampicin resistance, yomwe yakhala njira yodziwira matenda yomwe bungwe la WHO limalimbikitsa pochiza chifuwa chachikulu.^[3]. Ngakhale kupezeka kwa rifampicin kukana kumakhala kofanana ndi kupezeka kwa MDR-TB, kuzindikira kokha kukana kwa rifampicin kumanyalanyaza odwala omwe ali ndi mono-resistant INH (kutanthauza kukana kwa isoniazid koma kumva rifampicin) ndi mono-resistant rifampicin (kukhudzidwa kwa isoniazid koma kukana rifampicin), zomwe zingapangitse odwala kulephera kulandira chithandizo choyamba. Choncho, kuyezetsa kwa isoniazid ndi rifampicin ndizofunika zochepa pamapulogalamu onse oletsa DR-TB^[⁴^].

Technical Parameters

Kusungirako	≤-18 ℃
Alumali moyo	12 miyezi
Mtundu wa Chitsanzo	Zitsanzo za sputum, Chikhalidwe Chokhazikika (LJ Medium), Chikhalidwe Chamadzimadzi (MGIT Medium)
CV	<5.0%
LoD	LoD ya zida zodziwira Mycobacterium tuberculosis ndi 10 bacteria/mL;LoD ya zida zodziwira mtundu wa rifampicin wakuthengo ndi mtundu wa mutant ndi 150 bacteria/mL; LoD ya zida zodziwira mtundu wa isoniazid wakuthengo ndi mtundu wosinthika ndi 200 bacteria/mL.
Mwatsatanetsatane	1) Palibe njira yolumikizirana mukamagwiritsa ntchito zida kuti muzindikire DNA yamunthu (500ng), mitundu ina ya 28 ya tizilombo toyambitsa matenda opuma, ndi mitundu 29 ya mycobacteria yopanda tuberculous (monga momwe tawonetsera mu Gulu 3).2) Palibe kusintha kosiyanasiyana mukamagwiritsa ntchito zida kuti muzindikire malo osinthika amitundu ina ya rifampicin yosamva mankhwala ndi isoniazid sensitive Mycobacterium tuberculosis (monga momwe tawonetsera mu Gulu 4).3) Zinthu zomwe zimasokoneza nthawi zambiri m'zitsanzo zomwe zimayenera kuyesedwa, monga rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L0), dem pyrazinamide (45mg/L) (20mg/L) mankhwala, alibe zotsatira pa mayeso zida.
Zida Zogwiritsira Ntchito	SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Real-Time PCR System