Mycobacterium Tuberculosis INH Mutation
Dzina la malonda
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Mkhota Wosungunuka)
Epidemiology
Mycobacterium tuberculosis, yomwe imadziwika kuti Tubercle bacillus (TB), ndi tizilombo toyambitsa matenda timene timayambitsa chifuwa chachikulu. Panopa, ambiri ntchito mzere woyamba odana ndi TB mankhwala monga INH, rifampicin ndi hexambutol, etc. Mzere wachiwiri odana ndi TB mankhwala monga fluoroquinolones, amikacin ndi kanamycin, etc. Mankhwala atsopano opangidwa ndi linezolid, bedaquiline ndi delamani, etc. Komabe, chifukwa cha kugwiritsidwa ntchito molakwika kwa anti-TB komanso mawonekedwe a chifuwa chachikulu cha chifuwa chachikulu cha mycobacteriosis, ndi zina zotero. mycobacterium TB imayambitsa kukana kwa mankhwala ku mankhwala odana ndi TB, zomwe zimabweretsa zovuta zazikulu pakupewa ndi kuchiza chifuwa chachikulu.
Channel
| FAM | MP nucleic acid |
| Mtengo ROX | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | makoko |
| CV | ≤5% |
| LoD | Malire ozindikira mabakiteriya amtundu wa INH ndi 2x103 mabakiteriya/mL, ndipo malire ozindikira mabakiteriya osasinthika ndi 2x103 mabakiteriya/mL. |
| Mwatsatanetsatane | a. Palibe njira yolumikizirana pakati pa ma genome amunthu, mabakiteriya ena omwe sianthuberculous mycobacteria ndi chibayo omwe apezeka ndi zida izi.b. Malo osinthika a majini ena osamva mankhwala mumtundu wakuthengo wa Mycobacterium tuberculosis, monga momwe jini ya rifampicin rpoB imakana, adadziwika, ndipo zotsatira zoyesa zidawonetsa kuti palibe kukana kwa INH, zomwe zikuwonetsa kuti palibe kusinthananso. |
| Zida Zogwiritsira Ntchito | SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR SystemsLightCycler480®Real-Time PCR System |
Kuyenda Ntchito
Ngati mugwiritsa ntchito Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., L. sputum chitsanzo kuyesedwa motsatizana, ndi kuwonjezera 10μL ya ulamuliro mkati padera mu ulamuliro zoipa, kukonzedwa sputum chitsanzo kuyezetsa, ndi masitepe wotsatira ayenera mosamalitsa ikuchitika molingana ndi malangizo m'zigawo. Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 100μL.
