Kusintha kwa Mycobacterium Tuberculosis INH
Dzina la chinthu
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, yomwe imatchedwa Tubercle bacillus (TB), ndi mabakiteriya omwe amayambitsa chifuwa chachikulu. Pakadali pano, mankhwala oyamba oletsa chifuwa chachikulu omwe amagwiritsidwa ntchito kwambiri ndi INH, rifampicin ndi hexambutol, ndi zina zotero. Mankhwala achiwiri oletsa chifuwa chachikulu ndi fluoroquinolones, amikacin ndi kanamycin, ndi zina zotero. Mankhwala atsopano omwe apangidwa ndi linezolid, bedaquiline ndi delamani, ndi zina zotero. Komabe, chifukwa cha kugwiritsa ntchito molakwika mankhwala oletsa chifuwa chachikulu komanso mawonekedwe a khoma la maselo a mycobacterium tuberculosis, mycobacterium tuberculosis imakhala ndi kukana mankhwala ku mankhwala oletsa chifuwa chachikulu, zomwe zimabweretsa zovuta zazikulu popewa ndi kuchiza chifuwa chachikulu.
Njira
| FAM | MP nucleic acid |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | sputum |
| CV | ≤5% |
| LoD | Malire ozindikira mabakiteriya a mtundu wa INH wachilengedwe ndi 2x103 mabakiteriya/mL, ndipo malire ozindikira mabakiteriya osinthika ndi 2x103 mabakiteriya/mL. |
| Kufotokozera Mwapadera | a. Palibe kusinthana kwa majini a anthu, mabakiteriya ena osayambitsa chifuwa chachikulu komanso matenda a chibayo omwe apezeka ndi chida ichi.b. Malo osinthira majini ena osagwiritsa ntchito mankhwala mu Mycobacterium tuberculosis yamtundu wakuthengo, monga dera lomwe limazindikira kukana kwa jini ya rifampicin rpoB, adapezeka, ndipo zotsatira za mayeso sizinasonyeze kukana kwa INH, zomwe sizikusonyeza kuti pali kusintha kulikonse. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a SLAN-96P a Nthawi YeniyeniMachitidwe a PCR a BioRad CFX96 Real-TimeLightCycler480®Dongosolo la PCR la Nthawi Yeniyeni |
Kuyenda kwa Ntchito
Ngati mugwiritsa ntchito Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. kuti muchotse, onjezani 200μL ya negative control ndi sputum sample yokonzedwa kuti muyesedwe motsatizana, ndikuwonjezera 10μL ya internal control padera mu negative control, sputum sample yokonzedwa kuti muyesedwe, ndipo njira zotsatirazi ziyenera kuchitidwa mosamala motsatira malangizo ochotsera. Volume ya chitsanzo yochotsedwa ndi 200μL, ndipo volume yovomerezeka yochotsera ndi 100μL.
