Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Kufotokozera Kwachidule:

Chidachi ndi choyenera kuzindikira zamtundu waukulu wa masinthidwe amtundu wa sputum wa anthu omwe amasonkhanitsidwa kuchokera ku Tubercle bacillus positive odwala omwe amatsogolera ku mycobacterium tuberculosis isoniazid kukana: InhA promotioner region -15C>T, -8T>A, -8T>C;Chigawo cholimbikitsa cha AhpC -12C>T, -6G>A;kusintha kwa homozygous kwa KatG 315 codon 315G>A, 315G>C.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Dzina la malonda

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Mkhota Wosungunuka)

Epidemiology

Mycobacterium tuberculosis, yomwe imadziwika kuti Tubercle bacillus (TB), ndi tizilombo toyambitsa matenda timene timayambitsa chifuwa chachikulu.Pakalipano, mankhwala omwe amagwiritsidwa ntchito kwambiri pamzere woyamba wa chifuwa chachikulu ndi isoniazid, rifampicin ndi hexambutol, ndi zina zotero. Mankhwala amtundu wachiwiri oletsa chifuwa chachikulu ndi fluoroquinolones, amikacin ndi kanamycin, ndi zina zotero. Mankhwala atsopano opangidwa ndi linezolid, bedaquiline ndi delamani, ndi zina zotero. Komabe, chifukwa chogwiritsa ntchito molakwika mankhwala odana ndi chifuwa chachikulu komanso mawonekedwe a cell khoma la mycobacterium TB, mycobacterium TB imakulitsa kukana kwa mankhwala ku anti-TB, zomwe zimabweretsa zovuta zazikulu pakupewa ndi kuchiza chifuwa chachikulu.

Channel

FAM	MP nucleic acid
Mtengo ROX	Ulamuliro Wamkati

Magawo aukadaulo

Kusungirako	≤-18 ℃
Alumali moyo	12 miyezi
Mtundu wa Chitsanzo	makoko
CV	≤5%
LoD	Malire ozindikira mabakiteriya olimbana ndi isoniazid akuthengo ndi 2x103 mabakiteriya/mL, ndipo malire ozindikira mabakiteriya osasintha ndi 2x103 mabakiteriya/mL.
Mwatsatanetsatane	a.Palibe njira yolumikizirana pakati pa ma genome amunthu, mabakiteriya ena omwe sianthuberculous mycobacteria ndi chibayo omwe apezeka ndi zida izi.b.Malo osinthika a majini ena osamva mankhwala mumtundu wakuthengo wa Mycobacterium tuberculosis, monga momwe jini ya rifampicin rpoB imakanikira, adapezeka, ndipo zotsatira zoyesa zidawonetsa kuti palibe kukana kwa isoniazid, zomwe zikuwonetsa kuti palibe kusinthananso.
Zida Zogwiritsira Ntchito	SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR Systems LightCycler480® Real-Time PCR System

Kuyenda Ntchito

Ngati mugwiritsa ntchito Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. kuti muchotse, onjezani 200μL ya kuwongolera koyipa ndi kusinthidwa kwa sputum kuyesedwa motsatizana, ndikuwonjezera 10μL ya mkati ulamuliro payokha mu ulamuliro zoipa, kukonzedwa sputum chitsanzo kuyezetsa, ndi wotsatira masitepe ayenera mosamalitsa ikuchitika motsatira malangizo m'zigawo.Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 100μL.