Kachilombo ka Fuluwenza A/ Kachilombo ka Fuluwenza B
Dzina la chinthu
Kiti Yodziwira Kachilombo ka HWTS-RT174-Influenza A Virus/Influenza B Virus Nucleic Acid (Fluorescence PCR)
Epidemiology
Kutengera kusiyana kwa antigenic pakati pa jini la NP ndi jini la M, mavairasi a chimfine amatha kugawidwa m'mitundu inayi: kachilombo ka chimfine A (IFV A), kachilombo ka chimfine B (IFV B), kachilombo ka chimfine C (IFV C) ndi kachilombo ka chimfine D (IFV D).[1]Kachilombo ka Influenza A kali ndi mitundu yambiri ya tizilombo toyambitsa matenda komanso ma serotypes ovuta, ndipo amatha kufalikira pakati pa tizilombo toyambitsa matenda kudzera mu majini osinthika komanso kusintha kwa majini. Anthu alibe chitetezo chokhazikika ku kachilombo ka influenza A, kotero anthu azaka zonse nthawi zambiri amakhala pachiwopsezo. Kachilombo ka Influenza A ndi kachilombo kamene kamayambitsa miliri ya influenza[2]Kachilombo ka fuluwenza B kamapezeka kwambiri m'dera laling'ono ndipo pakadali pano palibe mitundu ina. Kachilombo kamene kamayambitsa matenda kwa anthu ndi ka mtundu wa B/Yamagata kapena ka mtundu wa B/Victoria. Pakati pa milandu yotsimikizika ya fuluwenza m'maiko 15 m'chigawo cha Asia-Pacific mwezi uliwonse, chiŵerengero chotsimikizika cha kachilombo ka fuluwenza B ndi 0-92%.[3]Mosiyana ndi kachilombo ka chimfine A, magulu enaake monga ana ndi okalamba ali pachiwopsezo chotenga kachilombo ka chimfine B ndipo amakhala pachiwopsezo chotenga mavuto, zomwe zimaika mtolo waukulu pagulu kuposa kachilombo ka chimfine A.[4].
Njira
| FAM | MP nucleic acid |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chitsanzo cha swab ya m'kamwa |
| Ct | Fuluwenza A, Fuluwenza BCt≤35 |
| CV | <5.0% |
| LoD | Fuluwenza A ndi Fuluwenza Bzonse ndi 200Copes/mL |
| Kufotokozera Mwapadera | Kuyanjana pakati pa zida ndi Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, human metapneumovirus, adenovirus, human coronavirus, novel coronavirus, SARS coronavirus, MERS coronavirus, rotavirus, norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, ndi DNA ya majini ya munthu. Mayeso oletsa kusokoneza: Sankhani mucin (60 mg/mL), magazi a munthu (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) yokhala ndi 5% yotetezera, beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L) kuti muyesedwe kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zosokoneza zomwe zili pamwambazi sizikusokoneza kuzindikira kwa zida. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la PCR la Applied Biosystems 7500 Real-Time QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.amalangizidwa kuti atenge zitsanzo ndinjira zotsatirazi ziyenera kutsatiridwamayendedweyakhazikitsidwa motsatira malamulo a IFUya Kit.
