Kusintha kwa majini a TEL-AML1 a anthu
Dzina la chinthu
HWTS-TM016 Human TEL-AML1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a khansa ya m'magazi (ALL) ndi khansa yofala kwambiri paubwana. M'zaka zaposachedwapa, khansa ya m'magazi (AL) yasintha kuchokera ku mtundu wa MIC (mawonekedwe, chitetezo chamthupi, cytogenetics) kupita ku mtundu wa MICM (kuwonjezera mayeso a biology ya molecular). Mu 1994, zidapezeka kuti kusakanikirana kwa TEL paubwana kudachitika chifukwa cha kusintha kwa chromosome t(12;21)(p13;q22) mu B-lineage acute lymphoblastic leukemia (ALL). Kuyambira pomwe jini ya AML1 fusion idapezeka, jini ya TEL-AML1 fusion ndiyo njira yabwino kwambiri yodziwira matenda a ana omwe ali ndi khansa ya m'magazi (acute lymphoblastic leukemia).
Njira
| FAM | Jini yosakanikirana ya TEL-AML1 |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | chitsanzo cha mafuta a m'mafupa |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | Makopi 1000/mL |
| Kufotokozera Mwapadera | Palibe kusinthana kwa majini pakati pa ma kits ndi majini ena osakanikirana monga BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Kiti Yochotsera RNA Yoyera ya Magazi Onse a RNA (DP433).
