Mtundu wa braf v600e

Kufotokozera kwaifupi:

Makina oyesererawa amagwiritsidwa ntchito pofuna kudziwa mtundu wa braf v600e mu minofu ya helaffin, khansa yapadera, chithokomiro cha chithokomiro, chithokomiro cham'mapapo ndi khansa yam'mapapo ku Vitro.


Tsatanetsatane wazogulitsa

Matamba a malonda

Dzina lazogulitsa

HWTS-TM007-TMI007-Churm Braf RAF VE600E Monning Col Kit (Fluorescence PCR)

Chiphaso

CE / tfda

Epidemiology

Mitundu yoposa 30 ya zakuthambo zapezeka, pomwe pafupifupi 90% ili ku Exon 15, pomwe V6IE Vanf wodziwika kwambiri, ndiye kuti, thkisi (T) pa Exon 15 imasinthidwa Adenine (a), zomwe zimapangitsa kuti birine (v) pa malo 600 ndi glutamic acid (e) mu mapuloteni. Braf matition nthawi zambiri amapezeka m'matumba oyipa monga khansa ya mankhwala, khansa ya chithokomiro, chithokomiro, ndi khansa yam'mapapo. Kuzindikira kusinthika kwa Braf Gene wakhala kofunikira kuwunika kwa EGFR-Tkis ndi Braf Morger Venters mankhwala ochiritsira mankhwala ochiritsira odwala omwe angaphunzitse odwala omwe angapindule.

Ngalande

Banja V6IE BREA, Kuwongolera kwamkati

Magawo aluso

Kusunga

≤-18 ℃

Alumali-Moyo

Miyezi 9

Mtundu wofanana

Paraffin-Combed-Combeld Minofu yaminyewa

CV

<5.0%

Ct

≤38

Lodu

Gwiritsani ntchito chipamwamba kuti mudziwe zowongolera zofananira. a) Pansi pa 3ng / μl kapena mtundu wa nthawi yosintha, 1% mabilidwe amatha kupezeka munthawi ya buffer; b) pansi pa nthawi ya madongosolo, kusinthika kwa 1 × 103Makope / ml mu mtundu wamtchire wa 1 × 105Makope / ml amatha kupezeka mu buffer; c) Kutengera kwa IC Buffer kumatha kuzindikira malire otsika kwambiri kuwongolera SW3 ya kampani yamkati.

Zida Zogwirizira:

Imagwiritsa ntchito ma biosyystems 7500 pcr PCR SystemsAmagwiritsa ntchito bioshasystems 7300 pcr

Makina, Anchtudio® 5 PCR Systems

Speccycler®480 PCR System

Biorad Cfx96 PCR PCR System

Kuyenda

Kulimbikitsidwa Kutulutsa: Qiagen's Qiagen DNA FFE FRAFT Clower (56404), minyewa yolumikizidwa ndi ma paraffin


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