Kusintha kwa Gene V600E ya Anthu
Dzina la chinthu
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Satifiketi
CE/TFDA
Epidemiology
Mitundu yoposa 30 ya kusintha kwa BRAF yapezeka, yomwe pafupifupi 90% ili mu exon 15, komwe kusintha kwa V600E kumaonedwa kuti ndi kusintha kofala kwambiri, ndiko kuti, thymine(T) yomwe ili pamalo 1799 mu exon 15 imasinthidwa kukhala adenine (A), zomwe zimapangitsa kuti valine (V) ilowe m'malo mwa 600 ndi glutamic acid (E) mu puloteni. Kusintha kwa BRAF kumapezeka kwambiri m'matenda oopsa monga melanoma, khansa ya m'matumbo, khansa ya chithokomiro, ndi khansa ya m'mapapo. Kumvetsetsa kusintha kwa jini la BRAF kwakhala kufunikira kowunika mankhwala a EGFR-TKIs ndi BRAF omwe amawunikira kusintha kwa majini m'mankhwala ochizira odwala omwe angapindule nawo.
Njira
| FAM | Kusintha kwa V600E, kulamulira kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | zitsanzo za minofu yodwala yomwe ili ndi parafini |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Gwiritsani ntchito zidazi kuti mupeze chiwongolero cha khalidwe la LoD chogwirizana. a) Pansi pa 3ng/μL wild-type background, 1% mutation rate ingapezeke mu reaction buffer mokhazikika; b) pansi pa 1% mutation rate, 1×103Makopi/mL kumbuyo kwa mtundu wachilengedwe wa 1×105Makopi/mL amatha kupezeka mokhazikika mu reaction buffer; c) IC Reaction Buffer imatha kuzindikira SW3 yotsika kwambiri yowongolera khalidwe la kampani. |
| Zida Zogwiritsidwa Ntchito: | Machitidwe a Biosystems 7500 a Real-Time PCRMapulogalamu Ogwiritsidwa Ntchito a Biosystems 7300 Real-Time PCR Machitidwe, QuantStudio® 5 Machitidwe a PCR a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo la PCR la BioRad CFX96 Real-Time |
Kuyenda kwa Ntchito
Ma reagents ofunikira ochotsera: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd.
