Mtundu wa braf v600e
Dzina lazogulitsa
HWTS-TM007-TMI007-Churm Braf RAF VE600E Monning Col Kit (Fluorescence PCR)
Chiphaso
CE / tfda
Epidemiology
Mitundu yoposa 30 ya zakuthambo zapezeka, pomwe pafupifupi 90% ili ku Exon 15, pomwe V6IE Vanf wodziwika kwambiri, ndiye kuti, thkisi (T) pa Exon 15 imasinthidwa Adenine (a), zomwe zimapangitsa kuti birine (v) pa malo 600 ndi glutamic acid (e) mu mapuloteni. Braf matition nthawi zambiri amapezeka m'matumba oyipa monga khansa ya mankhwala, khansa ya chithokomiro, chithokomiro, ndi khansa yam'mapapo. Kuzindikira kusinthika kwa Braf Gene wakhala kofunikira kuwunika kwa EGFR-Tkis ndi Braf Morger Venters mankhwala ochiritsira mankhwala ochiritsira odwala omwe angaphunzitse odwala omwe angapindule.
Ngalande
Banja | V6IE BREA, Kuwongolera kwamkati |
Magawo aluso
Kusunga | ≤-18 ℃ |
Alumali-Moyo | Miyezi 9 |
Mtundu wofanana | Paraffin-Combed-Combeld Minofu yaminyewa |
CV | <5.0% |
Ct | ≤38 |
Lodu | Gwiritsani ntchito chipamwamba kuti mudziwe zowongolera zofananira. a) Pansi pa 3ng / μl kapena mtundu wa nthawi yosintha, 1% mabilidwe amatha kupezeka munthawi ya buffer; b) pansi pa nthawi ya madongosolo, kusinthika kwa 1 × 103Makope / ml mu mtundu wamtchire wa 1 × 105Makope / ml amatha kupezeka mu buffer; c) Kutengera kwa IC Buffer kumatha kuzindikira malire otsika kwambiri kuwongolera SW3 ya kampani yamkati. |
Zida Zogwirizira: | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR SystemsAmagwiritsa ntchito bioshasystems 7300 pcr Makina, Anchtudio® 5 PCR Systems Speccycler®480 PCR System Biorad Cfx96 PCR PCR System |
Kuyenda
Kulimbikitsidwa Kutulutsa: Qiagen's Qiagen DNA FFE FRAFT Clower (56404), minyewa yolumikizidwa ndi ma paraffin