Herpes Simplex Virus Type 2 Nucleic Acid
Dzina lazogulitsa
HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)
Cholinga Chogwiritsidwa Ntchito
Chidachi chimagwiritsidwa ntchito pozindikira zamtundu wa herpes simplex virus mtundu 2 nucleic acid mu swab wamwamuna wa mkodzo ndi zitsanzo za khomo lachiberekero.
Epidemiology
Herpes Simplex Virus Type 2 (HSV2) ndi kachilombo kozungulira kopangidwa ndi tegument, capsid, core, ndi envelopu, ndipo imakhala ndi mizere iwiri ya DNA.Matenda a Herpes amatha kulowa m'thupi mwa kukhudzana mwachindunji kapena kugonana ndi khungu ndi mucous nembanemba, ndipo amagawidwa kukhala oyambirira komanso obwerezabwereza.Matenda a ubereki amayamba makamaka ndi HSV2, odwala amuna amawonetsedwa ngati zilonda za mbolo, ndipo odwala achikazi amawonetsedwa ngati zilonda zam'mimba, vulvar, ndi maliseche.The koyamba matenda a maliseche nsungu HIV nthawi zambiri recessive matenda, kupatula ochepa m`deralo nsungu ndi mucous nembanemba kapena khungu, ambiri amene alibe zizindikiro zachipatala zoonekeratu.Matenda a genital nsungu ali ndi makhalidwe a moyo wonse wonyamula kachilombo ka HIV ndi kubwereza kosavuta, ndipo odwala ndi onyamula ndi omwe amayambitsa matenda.Ku China, kuchuluka kwa serological positive kwa HSV2 kuli pafupifupi 10.80% mpaka 23.56%.Gawo la matenda a HSV2 likhoza kugawidwa kukhala matenda oyamba ndi matenda obwerezabwereza, ndipo pafupifupi 60% ya odwala omwe ali ndi kachilombo ka HSV2 amayambiranso.
Epidemiology
FAM: Herpes Simplex Virus Type 2 (HSV2)·
VIC(HEX): Kulamulira Kwamkati
PCR Amplification Conditions Kukhazikitsa
Khwerero | Zozungulira | Kutentha | Nthawi | SunganiFluorescentSmagalasikapena osati |
1 | 1 kuzungulira | 50 ℃ | 5 mins | No |
2 | 1 kuzungulira | 95 ℃ | 10 mins | No |
3 | 40 kuzungulira | 95 ℃ | 15mphindi | No |
4 | 58 ℃ | 31mphindi | Inde |
Magawo aukadaulo
Kusungirako | |
Madzi | ≤-18 ℃ Mumdima |
Alumali moyo | 12 miyezi |
Mtundu wa Chitsanzo | Mphepete mwa khomo lachiberekero lachikazi, Male swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Makope/machitidwe |
Mwatsatanetsatane | Palibe mtanda-reactivity ndi matenda ena opatsirana pogonana, monga Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium ndi etc. |
Zida Zogwiritsira Ntchito | Itha kufanana ndi zida za fulorosenti za PCR pamsika. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System. |