Kachilombo ka Hantaan Virus Nucleic
Dzina la chinthu
HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hantavirus ndi mtundu wa kachilombo ka RNA kozungulira, kogawanika, komanso kopanda ma strand. Hantavirus imagawidwa m'magulu awiri: imodzi imayambitsa Hantavirus pulmonary syndrome (HPS), ndipo inayo imayambitsa Hantavirus hemorrhagic fever yokhala ndi renal syndrome (HFRS). Yoyamba imapezeka kwambiri ku Europe ndi United States, ndipo yomaliza ndi hemorrhagic fever yokhala ndi renal syndrome yomwe imayambitsidwa ndi kachilombo ka Hantaan, komwe kamapezeka ku China. Zizindikiro za hantavirus hantaan type zimawonekera makamaka ngati hemorrhagic fever yokhala ndi renal syndrome, yomwe imadziwika ndi kutentha thupi kwambiri, hypotension, kutuluka magazi, oliguria kapena polyuria ndi zina zomwe zimayambitsa impso. Ndi matenda kwa anthu ndipo ayenera kusamalidwa mokwanira.
Njira
| FAM | hantavirus hantaan type |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | seramu watsopano |
| Ct | ≤38 |
| CV | ＜5.0% |
| LoD | Makopi 500/μL |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Ma reagents ofunikira ochotsera: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira IFU. Kuchuluka kwa chitsanzo chochotsera ndi 200μL. Kuchuluka kwa elution komwe kukulimbikitsidwa ndi 80μL.
Ma reagents ofunikira ochotsera: Nucleic Acid Extraction kapena Purification Kit (YDP315-R). Kuchotsa kuyenera kuchitika motsatira IFU. Kuchuluka kwa chitsanzo chochotsera ndi 140μL. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 60μL.
