Zaire wouma ndi wozizira komanso Sudan Ebolavirus Nucleic Acid
Dzina la chinthu
HWTS-FE035-Zaire ndi Sudan zouma ndi Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka Ebola ndi ka Filoviridae, komwe ndi kachilombo ka RNA kopanda magawo kamodzi. Mavairasi ndi ulusi wautali wokhala ndi kutalika kwa virion wapakati wa 1000nm ndi mainchesi pafupifupi 100nm. Jenomu ya kachilombo ka Ebola ndi kachilombo ka RNA kopanda magawo komwe kali ndi kukula kwa 18.9kb, komwe kamakhala ndi mapuloteni 7 omangira thupi ndi puloteni imodzi yosakhala yomangira thupi. Kachilombo ka Ebola kangagawidwe m'magulu monga Zaire, Sudan, Bundibugyo, Tai Forest ndi Reston. Pakati pawo, mtundu wa Zaire ndi mtundu wa Sudan zanenedwa kuti zimayambitsa imfa ya anthu ambiri chifukwa cha matenda. EHF (Ebola Hemorrhagic Fever) ndi matenda opatsirana opatsirana omwe amayambitsidwa ndi kachilombo ka Ebola. Anthu amadwala kwambiri akakhudza madzi amthupi, madzi otuluka m'thupi ndi ndowe za odwala kapena nyama zomwe zili ndi kachilomboka, ndipo zizindikiro zake zimakhala malungo, kutuluka magazi komanso kuwonongeka kwa ziwalo zingapo. EHF ili ndi chiwerengero chachikulu cha imfa cha 50%-90%. Pakadali pano, njira zodziwira matenda a Ebola ndi mayeso a labotale makamaka, kuphatikizapo mbali ziwiri: kuzindikira etiological ndi kuzindikira serological. Kuzindikira etiological kumaphatikizapo kuzindikira ma virus antigens m'magazi pogwiritsa ntchito ELISA, kuzindikira nucleic acids pogwiritsa ntchito njira zowonjezera monga RT-PCR, ndi zina zotero, ndikugwiritsa ntchito maselo Vero, Hela, ndi zina zotero kuti kachilombo kasapezeke komanso kakule. Kuzindikira serological kumaphatikizapo kuzindikira ma antibodies a Serum specific IgM mwa kutenga ELISA, ndi kuzindikira ma antibodies a Serum specific IgG pogwiritsa ntchito ELISA, immunofluorescence, ndi zina zotero.
Magawo aukadaulo
| Malo Osungirako | ≤30℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | seramu, plazitsanzo za sma |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Zida Zogwiritsidwa Ntchito | Ingagwiritsidwe ntchito pa reagent yozindikira mtundu woyamba: Machitidwe a Biosystems Ogwiritsidwa Ntchito 7500 Real-Time PCR, QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni, Makina a PCR a SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.), Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time (FQD-96A, ukadaulo wa Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Dongosolo la BioRad CFX96 Real-Time PCR, Dongosolo la BioRad CFX Opus 96 Real-Time PCR. Yogwiritsidwa ntchito pa reagent yozindikira mtundu wachiwiri: EudemonTMAIO800 (HWTS-EQ007) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kuyenda kwa Ntchito
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kuchotsa kuyenera kuchitika motsatira malangizo, ndipo kuchuluka kwa chitsanzo chochotsedwa ndi 200μL ndipo kuchuluka koyenera kwa elution ndi 80μL.







