Chlamydia Trachomatis yowuma mozizira
Dzina la malonda
HWTS-UR032C/D-Kuwumitsa-kuzizira kwa Chlamydia Trachomatis Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) ndi mtundu wa tizilombo tating'onoting'ono ta prokaryotic tomwe timakhala ndi parasitic m'maselo a eukaryotic.[1].Chlamydia trachomatis imagawidwa mu AK serotypes malinga ndi njira ya serotype.Urogenital thirakiti matenda makamaka amayamba ndi trachoma kwachilengedwenso mtundu DK serotypes, ndipo amuna makamaka anasonyeza urethritis, amene angathe kumasulidwa popanda mankhwala, koma ambiri a iwo amakhala aakulu, nthawi kukulirakulira, ndipo akhoza pamodzi ndi epididymitis, proctitis, etc.[2].Azimayi amatha kuyambitsidwa ndi urethritis, cervicitis, ndi zina zotero, ndi zovuta zina za salpingitis.[3].
Channel
FAM | Chlamydia trachomatis (CT) |
Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
Kusungirako | ≤30 ℃ |
Alumali moyo | 12 miyezi |
Mtundu wa Chitsanzo | Chophimba chachikazi cha khomo lachiberekero Amuna swab mkodzo Mkodzo wachimuna |
Tt | ≤28 |
CV | ≤10.0% |
LoD | 400 Makopi / ml |
Mwatsatanetsatane | palibe kuyanjananso pakati pa zidazi ndi tizilombo toyambitsa matenda a genitourinary tract monga omwe ali pachiwopsezo chachikulu cha Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasmapilogenic epilogenider , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei and human genomic DNA, etc. |
Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System ndi BioRad CFX Opus 96 Real-Time PCR System Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS-1600). |
Kuyenda Ntchito
Njira 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Kuchotsa kuyenera kuchitidwa motsatira IFU.Onjezani chitsanzo cha DNA chotengedwa ndi chotulutsa chotulutsa sampuli muzotchinga ndikuyesa chidacho mwachindunji, kapena zitsanzo zochotsedwa ziyenera kusungidwa pa 2-8 ℃ osapitilira maola 24.
Njira 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kuchotsa kuyenera kuchitidwa motsatira IFU, ndipo voliyumu yovomerezeka ndi 80μL.DNA yachitsanzo yotengedwa ndi njira ya maginito ya bead imatenthedwa pa 95 ° C kwa mphindi zitatu ndipo nthawi yomweyo imasambitsidwa ndi ayezi kwa mphindi ziwiri.Onjezani zitsanzo za DNA zomwe zakonzedwa muzotchinga zomwe zachitika ndikuyesa pa chipangizocho kapena zitsanzo zokonzedwa ziyenera kusungidwa pansi -18°C osapitilira miyezi inayi.Chiwerengero cha kuzizira kobwerezabwereza ndi kusungunuka sayenera kupitirira 4 kuzungulira.