Chlamydia Trachomatis youma mufiriji
Dzina la chinthu
HWTS-UR032C/D-Chida Chodziwira Chlamydia Trachomatis Nucleic Acid Chouma Chozizira (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) ndi mtundu wa tizilombo toyambitsa matenda ta prokaryotic tomwe timapezeka m'maselo a eukaryotic.[1]Matenda a Chlamydia trachomatis amagawidwa m'magulu a AK serotypes malinga ndi njira ya serotype. Matenda a m'mimba mwa akazi nthawi zambiri amayamba chifukwa cha trachoma biological variant DK serotypes, ndipo amuna nthawi zambiri amaonekera ngati urethritis, yomwe imatha kuchiritsidwa popanda chithandizo, koma ambiri mwa iwo amakhala osatha, nthawi zina amakula, ndipo amatha kuphatikizidwa ndi epididymitis, proctitis, ndi zina zotero.[2]Akazi amatha kuyambitsidwa ndi urethritis, cervicitis, ndi zina zotero, komanso mavuto ena akuluakulu a salpingitis.[3].
Njira
| FAM | Chlamydia trachomatis (CT) |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤30℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Nsalu ya pachibelekero cha mkazi Nsalu ya mkodzo ya amuna Mkodzo wa amuna |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Makope 400/mL |
| Kufotokozera Mwapadera | Palibe kusinthana kwa kachilomboka pakati pa zida izi ndi matenda ena opatsirana m'njira ya genitourinary monga mtundu wa papillomavirus wa munthu 16, mtundu wa papillomavirus wa munthu 18, mtundu wa kachilombo ka Herpes simplex Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, kachilombo ka immunodeficiency ka munthu, Lactobacillus casei ndi DNA ya genomic ya munthu, ndi zina zotero. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System ndi BioRad CFX Opus 96 Real-Time PCR System Dongosolo Losavuta la Kuzindikira Kuwala kwa Kuwala kwa Nthawi Yeniyeni()HWTS-1600）. |
Kuyenda kwa Ntchito
Njira 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kuchotsa kuyenera kuchitika motsatira malamulo a IFU. Onjezani DNA ya chitsanzo yotengedwa ndi sample release release reagent mu reaction buffer ndikuyesa pa chipangizocho mwachindunji, apo ayi zitsanzo zochotsedwa ziyenera kusungidwa pa 2-8℃ kwa maola osapitirira 24.
Njira yachiwiri.
Kiti ya DNA/RNA Yoyesera Macro ndi Micro-Test General (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kuchotsa kuyenera kuchitika motsatira IFU, ndipo kuchuluka koyenera kwa elution ndi 80μL. DNA ya chitsanzo yotulutsidwa ndi njira ya magnetic bead imatenthedwa pa 95°C kwa mphindi zitatu kenako nthawi yomweyo itsukidwe mu ayezi kwa mphindi ziwiri. Onjezani DNA ya chitsanzo yokonzedwa mu reaction buffer ndipo yesani pa chipangizocho kapena zitsanzo zokonzedwa ziyenera kusungidwa pansi pa -18°C kwa miyezi yosapitirira 4. Chiwerengero cha kuzizira ndi kusungunuka mobwerezabwereza sichiyenera kupitirira ma cycle anayi.
