Kachilombo ka Encephalitis ka m'nkhalango
Dzina la chinthu
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a encephalitis a m'nkhalango (FE), omwe amadziwikanso kuti nkhupakupa (nkhupakupa) ndi matenda opatsirana kwambiri a m'mitsempha yapakati omwe amayamba chifukwa cha kachilombo ka forest encephalitis. Kachilombo ka encephalitis ka m'nkhalango kali m'gulu la Flavivirus la banja la Flaviviridae. Tinthu ta tizilombo toyambitsa matenda ndi tozungulira ndipo m'mimba mwake ndi 40-50nm. Kulemera kwa molekyulu ndi pafupifupi 4×10.6Da, ndipo genome ya kachilomboka ndi RNA yokhala ndi positive-sense, yokhala ndi chingwe chimodzi[1]. Mwachipatala, imadziwika ndi kutentha thupi kwambiri, mutu, chikomokere, kuyabwa kwa meningeal mwachangu, komanso kufooka kwa minofu ya khosi ndi miyendo, ndipo imapha anthu ambiri. Kuzindikira msanga kachilombo ka encephalitis ya m'nkhalango ndiye chinsinsi cha chithandizo cha encephalitis ya m'nkhalango, ndipo kukhazikitsa njira yosavuta, yeniyeni komanso yachangu yodziwira matenda ndikofunikira kwambiri pakuwunika matenda a encephalitis ya m'nkhalango.[1,2].
Njira
| FAM | nthenda ya nucleic acid ya kachilombo ka encephalitis |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | seramu watsopano |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/mL |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chotsukira chomwe chikulangizidwa: Nucleic Acid Extraction kapena Purification Kit (YDP315-R) chopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd., kutsukira kuyenera kuchitika motsatira malangizo mosamala. Voliyumu yovomerezeka yotsukira chitsanzo ndi 140μL ndipo voliyumu yovomerezeka yotulutsira ndi 60μL.
Chotsukira chomwe chikufunidwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Kuchotsa kuyenera kuchitika motsatira malangizo mosamala. Voliyumu yovomerezeka yochotsa chitsanzo ndi 200μL ndipo voliyumu yovomerezeka yochotsera ndi 80μL.
