Dengue Virus, Zika Virus ndi Chikurunanya Virus
Dzina lazogulitsa
Hwts-fengue devis, ma virus a Zika ndi Chikunguny
Epidemiology
Denguue fever (DF), yomwe imaphatikizidwa ndi matenda a dengue (denv), ndi imodzi mwazomwezi ndi gawo lalikulu kwambiri la arbururul kwambiri matenda. Sing'anga yake yotumiza imaphatikizapo Aedes Aegy Aegygy Albopictus. DF imafala makamaka m'malo otentha komanso otentha. Denv ndi wa flavivizis pansi pa flaviviriirus flaviviriiida, ndipo imatha kukhala yolembedwa mu sewerolo 4 molingana ndi antigen wapamwamba. Mawonekedwe azachipatala amaphatikizapo kupweteka mutu, kutentha thupi, zofooka, kukulitsa kwa lymph node, kugwedezeka, kuvulala koopsa kapena kuvulaza milandu yambiri. M'zaka zaposachedwa, kusintha kwa nyengo, kutukuka kwam'mizinda, kukula kwa zinthu zokopa alendo ndi zinthu zina mwaperekatu zinthu mwachangu komanso kufalitsa df, kumapangitsa kukula kwa malo a mliri.
Ngalande
| Banja | Denv nuclectic acid |
| Rox | Kuwongolera kwamkati |
Magawo aluso
| Kusunga | -18 ℃ |
| Alumali-Moyo | Miyezi 9 |
| Mtundu wofanana | Seramu watsopano |
| Ct | ≤38 |
| CV | <5% |
| Lodu | 500 makope / ml |
| Chifanizo | Zotsatira Zoyeserera zikuwonetsa kuti pamene kuchuluka kwa birurubin ku seramu sikopitilira 168.2μMol / ml, ml kuzunzirako ku seramu sikopitilira 5mg / ml, palibe chifukwa cha dengue virus, zika virus kapena Chikuruna virus. Hepatitis, hepatitis b virus, kachilombo ka hepato crus, hantavirus ence ence ence entrus, ma virus a ku West National, ndipo zotsatira zake zikuwonetsa kuti palibe Kuzira pakati pa zinthu izi ndi tizilombo tomwe tafotokozazi. |
| Zida Zogwirizira | Ntchito za bioshasystems 7500 pcr Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Makupala®5 pcr yell pcr Kuwala®480 pcr pcr system Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Njira 1.
Tiams virus DNA / RNA Kit (YDP315-R), ndipo kuchotsera kuyenera kuchitika molingana ndi malangizo ogwiritsira ntchito. Buku la zitsanzo zoyendetsedwa ndi 140μL, ndi mafakitale olimbikitsidwa ndi 60μL.
Njira 2.
Macro & Micro-Direl DNA / RNA Kit (HWTS-3017-50, HWTS-3017-9, Gwts-3017-96) (yomwe itha kugwiritsidwa ntchito ndi macro-3017-96) (zomwe zingagwiritsidwe ntchito . Co., Ltd., ndipo kuchotsera uyenera kuchitidwa malinga ndi malangizo omwe mungagwiritse ntchito. Voliyumu yoyendetsedwa ndi 200μc, ndipo kuchuluka kwa Evest Evel ndi 80μl.
