Kachilombo ka Dengue, Kachilombo ka Zika ndi Chikungunya Virus Multiplex
Dzina la chinthu
HWTS-FE040 Dengue Virus, Zika Virus ndi Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a Dengue Fever (DF), omwe amayamba chifukwa cha matenda a dengue virus (DENV), ndi amodzi mwa matenda opatsirana a arbovirus omwe amafalikira kwambiri. Njira yake yofalitsira matendawa imaphatikizapo Aedes aegypti ndi Aedes albopictus. Matenda a DF amapezeka kwambiri m'madera otentha komanso otentha. Matenda a DENV ndi a flavivirus omwe ali pansi pa flaviviridae, ndipo amatha kugawidwa m'magulu anayi malinga ndi antigen pamwamba. Zizindikiro zachipatala za matenda a DENV zimaphatikizapo mutu, malungo, kufooka, kukulira kwa lymph node, leukopenia ndi zina zotero, komanso kutuluka magazi, kugwedezeka, kuvulala kwa chiwindi kapena imfa pazochitika zoopsa. M'zaka zaposachedwapa, kusintha kwa nyengo, kufalikira kwa mizinda, chitukuko chachangu cha zokopa alendo ndi zinthu zina zapereka njira zofulumira komanso zosavuta zofalitsira ndi kufalitsa matenda a DF, zomwe zimapangitsa kuti dera la DF lipitirire kukula.
Njira
| FAM | DENV nucleic acid |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | -18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Seramu watsopano |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Makope 500/mL |
| Kufotokozera Mwapadera | Zotsatira za mayeso oletsa zikusonyeza kuti pamene kuchuluka kwa bilirubin mu seramu sikupitirira 168.2μmol/ml, kuchuluka kwa hemoglobin komwe kumapangidwa ndi hemolysis sikupitirira 130g/L, kuchuluka kwa mafuta m'magazi sikupitirira 65mmol/ml, kuchuluka konse kwa IgG mu seramu sikupitirira 5mg/mL, palibe chomwe chimakhudza kachilombo ka dengue, kupezeka kwa kachilombo ka Zika kapena chikungunya. Kachilombo ka Hepatitis A, kachilombo ka Hepatitis B, kachilombo ka Hepatitis C, kachilombo ka Herpes, kachilombo ka Eastern equine encephalitis, Hantavirus, kachilombo ka Bunya, kachilombo ka West Nile ndi zitsanzo za seramu ya majini a anthu zimasankhidwa kuti ziyesedwe mosiyanasiyana, ndipo zotsatira zake zikusonyeza kuti palibe kusinthana pakati pa kit iyi ndi tizilombo toyambitsa matenda tatchulidwa pamwambapa. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), ndipo kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chochotsedwa ndi 140μL, ndipo kuchuluka koyenera kwa elution ndi 60μL.
Njira yachiwiri.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ndipo kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Voliyumu ya chitsanzo chochotsedwa ndi 200μL, ndipo voliyumu yovomerezeka ya elution ndi 80μL.
