Dengue Virus, Zika Virus and Chikungunya Virus Multiplex
Dzina la malonda
HWTS-FE040 Dengue Virus, Zika Virus and Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Dengue fever (DF), yomwe imayambitsidwa ndi matenda a dengue virus (DENV), ndi amodzi mwa matenda opatsirana kwambiri a arbovirus. Sing'anga yake yotumizira imaphatikizapo Aedes aegypti ndi Aedes albopictus. DF imapezeka kwambiri kumadera otentha komanso otentha. DENV ndi ya flavivirus pansi pa flaviviridae, ndipo akhoza m'gulu 4 serotypes malinga ndi antigen pamwamba. chipatala mawonetseredwe DENV matenda makamaka monga mutu, kutentha thupi, kufooka, kukulitsa mwanabele, leukopenia ndi etc., ndi magazi, mantha, kwa chiwindi kuvulala kapena imfa kwambiri. M'zaka zaposachedwa, kusintha kwa nyengo, kukula kwa mizinda, chitukuko chofulumira cha zokopa alendo ndi zinthu zina zapereka mikhalidwe yofulumira komanso yosavuta yofalitsira ndi kufalikira kwa DF, zomwe zikupangitsa kuti dera la mliri wa DF likukulirakulira.
Channel
| FAM | DENV nucleic acid |
| Mtengo ROX | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | -18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Seramu yatsopano |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Makopi / ml |
| Mwatsatanetsatane | Zotsatira zoyeserera zosokoneza zikuwonetsa kuti kuchuluka kwa bilirubin mu seramu sikupitilira 168.2μmol/ml, kuchuluka kwa hemoglobin komwe kumapangidwa ndi hemolysis sikupitilira 130g/L, kuchuluka kwa lipid m'magazi sikuposa 65mmol/ml, kuchuluka kwa IgG mu seramu sikuposa 5mg/mL, kuzindikirika kwa virus kapena chikungue kulibe kachilomboka. Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus ndi human genomic serum samples amasankhidwa kuti ayesedwe, ndipo zotsatira zake zikuwonetsa kuti palibe kusinthana pakati pa zida izi ndi tizilombo toyambitsa matenda tatchulazi. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
TINamp Virus DNA/RNA Kit (YDP315-R), ndipo kuchotsako kumayenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Voliyumu yachitsanzo yotengedwa ndi 140μL, ndipo voliyumu yovomerezeka ndi 60μL.
Njira 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HCTS-060B) & Micro-Test Med-Tech Co., Ltd., ndi kuchotsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito. Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
