Chlamydia Trachomatis, Neisseria Gonorrhoeae ndi Trichomonas vaginalis
Dzina la malonda
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae and Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) ndi mtundu wa tizilombo tating'onoting'ono ta prokaryotic tomwe timakhala ndi parasitic m'maselo a eukaryotic.Chlamydia trachomatis imagawidwa mu AK serotypes malinga ndi njira ya serotype.Urogenital thirakiti matenda makamaka amayamba ndi trachoma kwachilengedwenso mtundu DK serotypes, ndipo amuna makamaka anasonyeza urethritis, amene angathe kumasulidwa popanda mankhwala, koma ambiri a iwo amakhala aakulu, nthawi kukulirakulira, ndipo akhoza pamodzi ndi epididymitis, proctitis, etc.
Channel
FAM | Chlamydia trachomatis |
Mtengo ROX | Neisseria gonorrhoeae |
CY5 | Trichomonal vaginitis |
VIC/HEX | Ulamuliro Wamkati |
Magawo aukadaulo
Kusungirako | -18 ℃ |
Alumali moyo | 12 miyezi |
Mtundu wa Chitsanzo | Mphepo yam'mimba ya mkazi,Kusamba kwa mkazi,Amuna swab mkodzo |
Ct | ≤38 |
CV | <5% |
LoD | 400Makope/mL |
Mwatsatanetsatane | Palibe njira yolumikizirana ndi ma virus ena a STD kunja kwa zida zoyeserera, monga Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, etc. |
Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Zomwe zimalangizidwa m'zigawo: Pipette 1mL ya chitsanzo kuti iyesedwe ku 1.5mL ya DNase / RNase-free centrifuge chubu, centrifuge pa 12000rpm kwa mphindi 3, kutaya supernatant ndi kusunga mpweya.Onjezani 200µL ya saline wamba pamadzi kuti muyimitsenso.Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito.Voliyumu yotulutsidwa ndi 200µL, ndipo voliyumu yovomerezeka ndi 80µL.
Njira 2.
Zomwe zimalangizidwa m'zigawo: Nucleic Acid Extraction kapena Purification Kit (YDP302).The m'zigawo ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito.Voliyumu yovomerezeka ndi 80µL.