Chlamydia Trachomatis, Neisseria Gonorrhoeae ndi Trichomonas vaginalis
Dzina la chinthu
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae ndi Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) ndi mtundu wa tizilombo toyambitsa matenda ta prokaryotic tomwe timapezeka m'maselo a eukaryotic. Chlamydia trachomatis imagawidwa m'maselo a AK malinga ndi njira ya serotype. Matenda a m'mimba mwa akazi nthawi zambiri amayamba chifukwa cha ma serotype a trachoma biological variant DK, ndipo amuna nthawi zambiri amaonekera ngati urethritis, yomwe imatha kuchiritsidwa popanda chithandizo, koma ambiri mwa iwo amakhala osatha, nthawi zina amakula, ndipo amatha kuphatikizidwa ndi epididymitis, proctitis, ndi zina zotero.
Njira
| FAM | Matenda a Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | -18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Nsalu yochotsa mimba ya mkazi,Nsalu yochotsera ukazi ya akazi,Nsalu ya mkodzo ya amuna |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Makopi/mL |
| Kufotokozera Mwapadera | Palibe njira yolumikizirana ndi matenda ena opatsirana pogonana omwe sapezeka mu kit yoyesera, monga Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, ndi zina zotero. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Chotsukira chovomerezeka: Pipette 1mL ya chitsanzo chomwe chiyenera kuyesedwa ku chubu cha centrifuge cha DNase/RNase cha 1.5mL, centrifuge pa 12000rpm kwa mphindi zitatu, taya supernatant ndikusunga precipitate. Onjezani 200µL ya saline wamba ku precipitate kuti muyimitsenso. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito. Volume ya chitsanzo chochotsedwa ndi 200µL, ndipo volume yovomerezeka ya elution ndi 80µL.
Njira yachiwiri.
Chotsukira chomwe chimalimbikitsidwa: Nucleic Acid Extraction kapena Purification Kit (YDP302). Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 80µL.
