Geni Yotsutsa Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Dzina la chinthu
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescence PCR)
Epidemiology
Mankhwala opha tizilombo a Carbapenem ndi mankhwala opha tizilombo a β-lactam omwe sagwiritsidwa ntchito kawirikawiri omwe ali ndi mabakiteriya ambiri komanso mphamvu yamphamvu kwambiri yopha tizilombo toyambitsa matenda. Chifukwa cha kukhazikika kwake ku β-lactamase komanso poizoni wochepa, akhala amodzi mwa mankhwala opha tizilombo ofunikira kwambiri pochiza matenda oopsa a bakiteriya. Carbapenems ndi okhazikika kwambiri ku plasmid-mediated extended-spectrum β-lactamases (ESBLs), ma chromosome, ndi plasmid-mediated cephalosporinases (AmpC enzymes).
Njira
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Kulamulira Kwamkati | Kulamulira Kwamkati |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Makoswe, madera oyera, swab ya rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Kufotokozera Mwapadera | a) Chidachi chimazindikira ma referensi okhazikika a kampani, ndipo zotsatira zake zimakwaniritsa zofunikira za ma referensi ogwirizana. b) Zotsatira za mayeso obwerezabwereza zikusonyeza kuti chida ichi sichinagwirizane ndi matenda ena opatsirana popuma, monga Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kapena zitsanzo zomwe zili ndi majini ena osagwira mankhwala a CTX, mecA, SME, SHV, TEM, ndi zina zotero. c) Mankhwala oletsa kusokoneza: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin amasankhidwa kuti ayesere kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zomwe zatchulidwa pamwambapa sizikusokoneza kuzindikira majini otsutsa carbapenem KPC, NDM, OXA48, OXA23, VIM, ndi IMP. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira Ma PCR a LineGene 9600 Plus Real-Time (FQD-96A，HangzhouUkadaulo wa Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Reagent yotulutsira yomwe ikulangizidwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Onjezani 200μL ya saline wamba ku thallus precipitate. Njira zotsatirazi ziyenera kutsatira malangizo ochotsera, ndipo kuchuluka kwa elution komwe kumalimbikitsidwa ndi100μL.
Njira yachiwiri.
Chothandizira kuchotsa chomwe chikulangizidwa: Nucleic Acid Extraction or Purification Reagent (YDP302) ndi Tiangen Biotech (Beijing) Co., Ltd. Kuchotsa kuyenera kuyambika motsatira gawo lachiwiri la malangizo ogwiritsira ntchito (onjezerani 200μL ya buffer GA ku thallus precipitate, ndikugwedeza mpaka thallus itayimitsidwa kwathunthu). Gwiritsani ntchito madzi opanda RNase/DNase kuti muchotse, ndipo voliyumu yochotsera yomwe ikulangizidwa ndi 100μL.
Njira 3.
Chotsukira chomwe chikulangizidwa: Chotsukira cha Macro & Micro-Test Sample Release Reagent. Chitsanzo cha sputum chiyenera kutsukidwa powonjezera 1mL ya saline wamba ku thallus precipitate yomwe yatchulidwa pamwambapa, ndikuyikidwa mu centrifuge pa 13000r/min kwa mphindi 5, ndipo supernatant imatayidwa (sungani 10-20µL ya supernatant). Kuti mupeze colony yoyera ndi rectal swab, onjezani 50μL ya sample release reagent mwachindunji ku thallus precipitate yomwe yatchulidwa pamwambapa, ndipo njira zotsatirazi ziyenera kuchotsedwa malinga ndi malangizo ogwiritsira ntchito.
