Kutsutsa kwa carbapenem
Dzina lazogulitsa
HWTS-OT045 Carbapenem Run (KPC / NDM / OXA 48 / Oxa 23 / Oxa 23
Epidemiology
Maantibayotiki a carbapenem ndi aypical β-lacticam maantibayotiki ndi antibacterial wofatsa komanso wamphamvu wa antibacterial. Chifukwa cha kukhazikika kwake kwa β-lactamase ndi zoopsa zochepa, zakhala imodzi mwa mankhwala ofunika kwambiri antibacterice othandizira bapiteri. Carbapenems imakhazikika kwambiri ku plasmid-yolumikizidwa-yactaming β-lactamases (esblosomes), ma chsmosomes, ndi ma cesmid-cepmid-cepmisosmes (ampr enzymes).
Ngalande
| PCR-Sakanizani 1 | PCR-Sakanizani 2 | |
| Banja | Engoli | Vim |
| Vic / Hex | Kuwongolera kwamkati | Kuwongolera kwamkati |
| Cy5 | Ndm | Kpc |
| Rox | Oxa48
| Oxa23 |
Magawo aluso
| Kusunga | ≤-18 ℃ |
| Alumali-Moyo | Miyezi 12 |
| Mtundu wofanana | Sputum, madera angwiro, swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lodu | 103CFU / ml |
| Chifanizo | a) Kit Ino imazindikira kuti kampaniyo idasiyidwa, ndipo zotsatira zake zikwaniritse zofunikira zomwe zikugwirizana. b) Zotsatira za kuyesa kwa mtanda zikuwonetsa kuti zida izi zilibe gawo linalake, monga klebanoboccus baumanniae, staudocococcus aerunguae, staudococccus barusi, Haemophilus influnzae, acninothobacter Junii, Acnidobacter konse Meca, SM, Shv, tem, etc. c) anti-kulowerera: Mucin, minocycline, clavimycin, rovomnem, cevoperazomycin, rovoperamycin, a cevoperamycin, a cevoperazone Kufufuza kwa Kutsutsa kwa carbapenem |
| Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Ma pcr-5 a PCR Slan-96p Real PCR Systems (Hongshi Chaurchirol Technology Co., Ltd.) Speccycler®480 PCR System Stonegene 9600 kuphatikiza njira zenizeni za PCR yowunikira (Fqd-96a,HangizhouUkadaulo wa Bioer) Ma-6000 enieni ophatikizira cycler (Suzhou Molarray Colarray Colary Colarray Colary Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Cor Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Njira 1.
Kulimbikitsidwa Kwambiri: Macro & Micro-Seled DNA / RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-Arm) Thallus urlus. Njira zotsatila ziyenera kutsata malangizowo pochotsera, ndipo kuchuluka kwa elery100μl.
Njira 2.
Kulimbikitsidwa komwe kumalimbikitsa: ecleic ya acidiclection kapena kuyeretsa (ydp302) ndi ma piijin), ltd. , ndikugwedezeka mpaka thaluluyi kuyimitsidwa kwathunthu). Gwiritsani ntchito rdase / dnase madzi aulere a erlution, ndipo kuchuluka kwa evest excomed ndi 100μ.
Njira 3.
Kulimbikitsidwa Kutulutsa: Macro & Mayeso oyeserera omasulira. Zitsanzo za sputum zimafunikira kutsukidwa ndi kuwonjezera 1Ml ya mchere wambiri ku Thallingo wotchulidwa pamwambapa, pa 13000r / mphindi kwa mphindi 5, ndipo superblele wa supernat). Kwa swab yangwiro ndi swab yoyenerera, onjezerani 50μl ya zitsanzo zomasulidwa mwachindunji ku thallus yotchulidwa pamwambapa, ndipo njira zotsatiridwa ziyenera kutulutsidwa malinga ndi malangizo omwe mungagwiritse ntchito.
