Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Dzina la malonda
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kit (Fluorescence PCR)
Epidemiology
Maantibayotiki a Carbapenem ndi ma β-lactam osawoneka bwino omwe ali ndi antibacterial yotakata kwambiri komanso antibacterial amphamvu kwambiri.Chifukwa cha kukhazikika kwake ku β-lactamase komanso kuchepa kwa kawopsedwe, yakhala imodzi mwamankhwala ofunikira kwambiri ochizira matenda oopsa a bakiteriya.Carbapenems ndi okhazikika kwambiri ku plasmid-mediated extended-spectrum β-lactamases (ESBLs), ma chromosome, ndi plasmid-mediated cephalosporinases (AmpC enzymes).
Channel
| Chithunzi cha PCR-Mix 1 | Chithunzi cha PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Ulamuliro Wamkati | Ulamuliro Wamkati |
| CY5 | NDM | KPC |
| Mtengo ROX | OXA48
| OXA23 |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Sputum, m'matumbo oyera, swab ya rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Mwatsatanetsatane | a) Chidacho chimazindikira mafotokozedwe olakwika a kampani, ndipo zotsatira zake zimakwaniritsa zofunikira zomwe zimafanana. b) Zotsatira za mayeso a cross-reactivity zikuwonetsa kuti chidachi sichimayenderana ndi ma virus ena opumira, monga Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Haephilus aureus, Klexytobacinella, Klexytobacinella, Klexytobacinella oreus, Klexytobacinella junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, kapena zitsanzo zomwe zili ndi mitundu ina yosamva mankhwala CTX, mecVA, TEM, etc. c) Kuletsa kusokoneza: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin amasankhidwa kuti ayesetse kusokoneza, ndipo zotsatira zimasonyeza kuti zinthu zosokoneza zomwe tatchulazi zilibe zosokoneza. kuti azindikire mitundu ya carbapenem yotsutsa KPC, NDM, OXA48, OXA23, VIM, ndi IMP. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A,Hangzhouukadaulo wa bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Zopangira zopangira zovomerezeka: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200μL ya saline wamba ku mphamvu ya thallus.Masitepe wotsatira ayenera kutsatira malangizo m'zigawo, ndi analimbikitsa elution voliyumu ndi100μl pa.
Njira 2.
Zomwe zimalangizidwa m'zigawo: Nucleic Acid Extraction kapena Purification Reagent (YDP302) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd. Kuchotsa kuyenera kuyambika motsatira gawo lachiwiri la malangizo oti mugwiritse ntchito (onjezani 200μL ya buffer GA ku mphepo yamkuntho. , ndi kugwedeza mpaka thallus itayimitsidwa kwathunthu).Gwiritsani ntchito madzi aulere a RNase/DNase kuti mumve zambiri, ndipo voliyumu yovomerezeka ndi 100μL.
Njira 3.
Zomwe zimalimbikitsidwa m'zigawo: Macro & Micro-Test Sample Release Reagent.Chitsanzo cha sputum chiyenera kutsukidwa powonjezera 1mL ya saline wamba ku thallus precipitate yomwe yatchulidwa pamwambapa, centrifuged pa 13000r/min kwa mphindi 5, ndipo chapamwamba chimatayidwa (sungani 10-20µL ya supernatant).Pazakudya zoyera komanso zathanzi, onjezani 50μL ya zotulutsa zotulutsa zomwe zatchulidwa pamwambapa, ndipo masitepe otsatirawa atengedwe molingana ndi malangizo ogwiritsira ntchito.
