Kuchuluka kwa majini a ALDH
Dzina la chinthu
HWTS-GE015Kiti Yodziwira Kuchuluka kwa Majini a ALDH (ARMS -PCR)
Epidemiology
Jini la ALDH2 (acetaldehyde dehydrogenase 2), limapezeka pa chromosome ya munthu 12. ALDH2 ili ndi esterase, dehydrogenase ndi reductase activity nthawi imodzi. Kafukufuku wasonyeza kuti ALDH2 ndi enzyme ya kagayidwe kachakudya ya nitroglycerin, yomwe imasintha nitroglycerin kukhala nitric oxide, motero imamasula mitsempha yamagazi ndikukonza mavuto oyenda magazi. Komabe, pali ma polymorphisms mu jini la ALDH2, omwe makamaka amapezeka ku East Asia. ALDH2*1/*1 GG ya mtundu wachilengedwe ili ndi mphamvu yayikulu ya kagayidwe kachakudya, pomwe mtundu wa heterozygous uli ndi 6% yokha ya ntchito ya enzyme ya mtundu wachilengedwe, ndipo mtundu wa homozygous mutant uli ndi pafupifupi ntchito ya enzyme, pomwe kagayidwe kachakudya kali kofooka kwambiri ndipo sikungathe kukwaniritsa zomwe mukufuna, zomwe zimapangitsa kuvulaza thupi la munthu.
Njira
| FAM | ALDH2 |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Magazi oletsa magazi kuundana a EDTA |
| CV | <5.0％ |
| LoD | 103Makopi/mL |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Ma reagents ofunikira ochotsera: gwiritsani ntchito Blood Genome DNA Extraction Kit (DP318) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. kapena Blood Genome Extraction Kit (A1120) yopangidwa ndi Promega kuti muchotse EDTA yoletsa magazi kuundana.
Ma reagents ofunikira ochotsera: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo mosamala. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi100μL.
