Mitundu 4 ya Ma virus Opumira
Dzina la malonda
HWTS-RT099- 4 Mitundu ya Ma virus Opumira Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a Corona Virus 2019, omwe amatchedwa "COVID-19", amatanthauza chibayo chomwe chimayambitsa2019-nCoVmatenda.2019-nCoVndi coronavirus wamtundu wa β.COVID-19 ndi matenda opatsirana kwambiri opatsirana, ndipo anthu nthawi zambiri amatengeka.Pakali pano, gwero la matenda makamaka odwala ndi2019-nCoV, ndipo anthu opanda zizindikiro angakhalenso magwero a matenda.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1-14, makamaka masiku 3-7.Kutentha thupi, chifuwa chowuma ndi kutopa ndizo zizindikiro zazikulu.Odwala ochepa anali ndi zizindikiros mongakutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsegula m'mimba, ndi zina.
Channel
| FAM | 2019-nCoV |
| VIC (HEX) | RSV |
| CY5 | IFV A |
| Mtengo ROX | IFV B |
| NED | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | -18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLInfluenza A virus/Influenza B virus/Respiratory syncytial virus: 500Copies/mL |
| Mwatsatanetsatane | a) Zotsatira zaposachedwa zikuwonetsa kuti palibe kulumikizana pakati pa zida ndi coronavirus yamunthu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus yaumunthu, enterovirus A, B, C, D, kachilombo ka anthu am'mapapo, epstein-barr virus, chikuku, kachilombo ka cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium chifuwa chachikulu, utsi aspergillus, candida albicans, candida glabrata glabrata chibayo ndi pnecoccus genocicoccus gerobe ndi pnecoccus gerococcus pneucocus, pnecoccus pneucoccus ndi pnecoccus pneucoccus. b) Kutha kusokoneza: sankhani mucin (60mg/mL), 10% (v/v) magazi ndi phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (kuphatikiza zoteteza) (20 mg/mL) beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL) Histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ndi tobramycin (0.6mg/mL) mL) poyesa kusokoneza, ndipo zotsatira zake zikuwonetsa kuti zinthu zosokoneza zomwe zatchulidwa pamwambapa sizisokoneza zotsatira za mayeso a tizilombo toyambitsa matenda. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems |
Kuyenda Ntchito
Njira 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) yopangidwa ndi Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. Voliyumu yotengedwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
Njira 2.
QIAamp Viral RNA Mini Kit (52904) yopangidwa ndi QIAGEN kapena Nucleic Acid Extraction or Purification Kit (YDP315-R) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. Voliyumu yotengedwa ndi 140μL, ndipo voliyumu yovomerezeka ndi 60μL.
