Mitundu 4 ya Mavairasi Opumira
Dzina la chinthu
HWTS-RT099- Mitundu 4 ya Mavairasi Opumira Nucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ABI 7500 Machitidwe a PCR Ofulumira Nthawi Yeniyeni/QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni
Mitundu ya HWTS-RT158-4 ya Mavairasi Opumira Nucleic Acid Detection Kit (Fluorescence PCR）-内参Quasar 705
Epidemiology
Matenda a Corona Virus 2019, omwe amatchedwa "COVID-19", amatanthauza chibayo chomwe chimayambitsidwa ndi2019-nCoVmatenda.2019-nCoVndi kachilombo ka corona komwe kali m'gulu la β. COVID-19 ndi matenda opatsirana opatsirana m'mapapo, ndipo anthu ambiri amakhala pachiwopsezo. Pakadali pano, magwero a matendawa ndi odwala omwe ali ndi kachilomboka2019-nCoV, ndipo anthu omwe ali ndi kachilomboka omwe alibe zizindikiro angakhalenso magwero a matendawa. Kutengera kafukufuku wamakono wa matenda, nthawi yoberekera ndi masiku 1-14, makamaka masiku 3-7. Malungo, chifuwa chouma ndi kutopa ndiye zizindikiro zazikulu. Odwala ochepa anali ndi zizindikiromongamphuno yodzaza, mphuno yotuluka madzi, pakhosi lopweteka, myalgia ndi kutsegula m'mimba, ndi zina zotero.
Njira
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | -18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Chidutswa cha m'kamwa |
| Ct | ≤38 |
| LoD | 2019-nCoV: Makopi 300/mLKachilombo ka Influenza A/kachilombo ka Influenza B/kachilombo ka kupuma kogwirizana ndi mpweya: 500Kopi/mL |
| Kufotokozera Mwapadera | a) Zotsatira za kafukufuku wosiyanasiyana zikuwonetsa kuti palibe kusinthana pakati pa zida ndi kachilombo ka corona ka anthu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. b) Mphamvu yoletsa kusokoneza: select mucin (60mg/mL), 10% (v/v) ya magazi ndi phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (kuphatikiza zosungira) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ndi tobramycin (0.6mg/mL) kuti muyesedwe kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zosokoneza zomwe zatchulidwa pamwambapa sizisokoneza zotsatira za mayeso a tizilombo toyambitsa matenda. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-TimeMachitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni |
Kuyenda kwa Ntchito
Njira 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL, ndipo kuchuluka koyenera kwa elution ndi 80μL.
Njira yachiwiri.
QIAamp Viral RNA Mini Kit (52904) yopangidwa ndi QIAGEN kapena Nucleic Acid Extraction kapena Purification Kit (YDP315-R) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. Kuchuluka kwa chitsanzo chomwe chatulutsidwa ndi 140μL, ndipo kuchuluka koyenera kwa elution ndi 60μL.
