Mitundu 17 ya HPV (16/18/6/11/44 Kulemba)
Dzina la malonda
HWTS-CC015 17 Mitundu ya Human Papillomavirus (16/18/6/11/44 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Khansara ya khomo pachibelekeropo ndi imodzi mwa zotupa zowopsa zomwe zimapezeka kwambiri m'njira zaubereki.Zasonyezedwa kuti matenda a HPV osatha komanso matenda angapo ndi chimodzi mwazinthu zazikulu zomwe zimayambitsa khansa ya pachibelekero.Pakali pano palibe chithandizo chovomerezeka cha khansa ya pachibelekero choyambitsidwa ndi HPV.Choncho, kuzindikira msanga ndi kupewa matenda a khomo pachibelekero chifukwa cha HPV ndi makiyi a kupewa khansa ya pachibelekeropo.Kukhazikitsidwa kwa mayeso osavuta, achindunji komanso othamanga mwachangu kwa tizilombo toyambitsa matenda ndikofunikira kwambiri pakuzindikira matenda a khansa ya pachibelekero.
Channel
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| Mtengo ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ulamuliro wamkati |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| Mtengo ROX | 44 | |
| CY5 | Ulamuliro wamkati |
Magawo aukadaulo
| Kusungirako | -18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Zitsanzo za mkodzo, chitsanzo cha swab ya khomo lachiberekero lachikazi, chitsanzo cha kumaliseche kwa mkazi |
| Ct | ≤28 |
| LoD | 300 Makopi / ml |
| Mwatsatanetsatane | Palibe cross-reactivity ndi Ureaplasma urealyticum, Chlamydia trachomatis of reproductive tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella ndi mitundu ina ya HPV yosaphimbidwa ndi zida. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Zomwe zimalangizidwa m'zigawo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu sitepe 2.1, ndiyeno kuchotsa kuyenera kuchitidwa molingana ndi ku malangizo ogwiritsira ntchito reagent iyi.Voliyumu yovomerezeka ndi 80μL.
Zopangira zopangira zovomerezeka: QIAamp DNA Mini Kit (51304) kapena Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Onjezerani 200μL ya saline wamba kuti muyikenso pellet mu sitepe 2.1, ndiyeno kuchotsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito reagent yochotsa izi.Voliyumu yotulutsidwa ya zitsanzo zonse ndi 200μL, ndipo voliyumu yovomerezeka ndi 100μL.
Alangizidwa m'zigawo reagent: Macro & Micro-Test Chitsanzo Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Onjezerani 200μL ya reagent yotulutsidwa kuti muyimitsenso pellet mu sitepe 2.1, ndiyeno kuchotsa kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito reagent iyi.
