Mitundu 17 ya HPV (16/18/6/11/44 Kulemba)
Dzina lazogulitsa
Hwts-cc015 Mitundu ya anthu ya papillomavirus (16/18/6/11/44 Kulemba) Nyukiti ya Nucleic Asic (Fluorescence PCR)
Epidemiology
Khansa yazenera ndi imodzi mwazotupa zonyansa kwambiri mu kapepalaka. Zawonetsedwa kuti matenda okhazikika a HPV ndi matenda angapo ndi amodzi mwazifukwa zazikulu za khansa ya khomo. Pakadali pano pali kusowa kwa mankhwala okwanira nthawi zambiri ku khansa ya khomo yoyambitsidwa ndi HPV. Chifukwa chake, kuzindikira koyambirira komanso kupewa matenda am'mimba oyambitsidwa ndi HPV ndi makiyi oletsa kupewa khansa yam'pongwe. Kukhazikitsidwa kwa mayeso osavuta, achindunji komanso mwachangu kwa tizilombo toyambitsa matenda ndi yofunika kwambiri pakuzindikira khansa ya khosi.
Ngalande
| PCR-Dint1 | Banja | 18 |
| Vic / Hex | 16 | |
| Rox | 31,35,35,39,51,52,58,59,66,66,68,68 | |
| Cy5 | Kuwongolera kwamkati |
| PCR-CAST2 | Banja | 6 |
| Vic / Hex | 11 | |
| Rox | 44 | |
| Cy5 | Kuwongolera kwamkati |
Magawo aluso
| Kusunga | -18 ℃ |
| Alumali-Moyo | Miyezi 12 |
| Mtundu wofanana | Mkhalidwe wa mkodzo, ndulu ya akazi achikazi, swab ya akazi |
| Ct | ≤28 |
| Lodu | 300Copees / ml |
| Chifanizo | Palibe chofiyira ndi Ureaplasma Ureafilma, Chlamydia trachimatis ya kubereka, albida albicans, neismonos vaginayis, ma trichomonas mitundu ina ya HPV sinaphimbidwa ndi zida. |
| Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Makupala®5 pcr yell pcr Slan-96P yeniyeni PCR Systems Kuwala®480 pcr pcr systems Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Kulimbikitsidwa Kwambiri: Macro & Bigro-Chefre DNA / RNA-3017-50, HWTS-3017-9, 3017-96) (yomwe ingayesedwe mayeso Zovala za Nucleut Acid & Micro-Tech Coun-Tech Co., ltd. Buku lolimbikitsidwa ndi 80μl.
Kulimbikitsidwa Kutulutsa: Qiamp DNA Mini Kit (51304) kapena macro-micro-mayeso oyeserera a Macro & RNA (HWTTS-3020-50). Onjezani milidi ya 200μl kuti muchepetse pellet mu Gawo 2.1, kenako kuchotsera kuyenera kuchitidwa malinga ndi malangizo omwe amagwiritsidwa ntchito pochotsera ichi. Zitsanzo zowonjezereka za 200μc, ndi kuchuluka kwa Evest Evel ndi 100μL.
Kulimbikitsidwa Kutulutsa: Macro & Macro-Chemile Kutulutsa (Hwts-3005-8I, 3005-8K, HWTS-3005-8L). Onjezani 200μL ya kukweza kwa chikondwerero kuti athetse pellet mu Gawo 2.1, kenako kuchotsera kuyenera kuchitika malinga ndi malangizo omwe amagwiritsa ntchito.
