Mitundu 17 ya HPV (16/18/6/11/44 Kulemba)
Dzina la chinthu
HWTS-CC015 Mitundu 17 ya kachilombo ka papilloma ka anthu (16/18/6/11/44 Kulemba) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Khansa ya pachibelekero ndi imodzi mwa zotupa zoopsa kwambiri m'njira yoberekera ya akazi. Zawonetsedwa kuti matenda a HPV osatha komanso matenda ambiri ndi chimodzi mwa zifukwa zazikulu za khansa ya pachibelekero. Pakadali pano palibe njira zovomerezeka zochiritsira khansa ya pachibelekero yomwe imayambitsidwa ndi HPV. Chifukwa chake, kuzindikira msanga ndi kupewa matenda a pachibelekero omwe amayambitsidwa ndi HPV ndiye njira zopewera khansa ya pachibelekero. Kukhazikitsa mayeso osavuta, enieni komanso ofulumira ozindikira matenda ndikofunikira kwambiri pakupeza matenda a pachibelekero.
Njira
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Kulamulira kwamkati |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Kulamulira kwamkati |
Magawo aukadaulo
| Malo Osungirako | -18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chitsanzo cha mkodzo, chitsanzo cha swab ya chiberekero cha akazi, chitsanzo cha swab ya chiberekero cha akazi |
| Ct | ≤28 |
| LoD | Makope 300/mL |
| Kufotokozera Mwapadera | Palibe njira yolumikizirana pakati pa Ureaplasma urealyticum, Chlamydia trachomatis of reproductive tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella ndi mitundu ina ya HPV yomwe siili m'gulu la mankhwala. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Machitidwe 480 a PCR a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu gawo 2.1, kenako kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chothandizira ichi chochotsera. Kuchuluka koyenera kwa elution ndi 80μL.
Chotsukira chomwe chikufunidwa: QIAamp DNA Mini Kit (51304) kapena Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu gawo 2.1, kenako kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chotsukira ichi. Kuchuluka kwa zitsanzo zomwe zachotsedwa ndi 200μL, ndipo kuchuluka kwa zotsukira komwe kukufunidwa ndi 100μL.
Chotsukira chomwe chikulangizidwa: Chotsukira cha Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Onjezani 200μL ya chotsukira cha sample release kuti muyimitsenso pellet mu gawo 2.1, kenako kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chotsukira ichi.
