Mitundu 14 ya Papillomavirus Yowopsa Kwambiri Yamunthu (16/18/52 Kulemba)
Dzina la malonda
HWTS-CC019A-Mitundu 14 ya Vuto Lowopsa la Human Papillomavirus (16/18/52 Typing) Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Kafukufuku wasonyeza kuti matenda a HPV osalekeza komanso matenda angapo ndi chimodzi mwazomwe zimayambitsa khansa ya pachibelekero.Pakadali pano, chithandizo chodziwika bwino cha khansa ya khomo pachibelekeropo choyambitsidwa ndi HPV sichinapezeke, chifukwa chake kudziwa msanga komanso kupewa matenda a khomo pachibelekero chifukwa cha HPV ndiye chinsinsi chopewera khansa ya pachibelekero.Ndikofunikira kwambiri kukhazikitsa mayeso osavuta, enieni komanso ofulumira a etiology kuti adziwe matenda ndi chithandizo cha khansa ya pachibelekero.
Channel
| Channel | Mtundu |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| Mtengo ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Mtengo wa 705/CY5.5 | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Mkodzo, Khomo la Khomo, Kumaliseche |
| Ct | ≤28 |
| LoD | 300 Makopi / ml |
| Mwatsatanetsatane | Palibe kupatsirana kwapang'onopang'ono ndi zitsanzo zina zopumira monga Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneuvirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
| Zida Zogwiritsira Ntchito | MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System ndi BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
1.Chitsanzo cha mkodzo
A: Tengani1.4mililita ya mkodzo chitsanzo kuyesedwa ndi centrifuge pa 12000rpm kwa mphindi 5;kutaya supernatant (ndi bwino kusunga 10-20μL supernatant kuchokera pansi pa chubu centrifuge), kuwonjezera 200μL chitsanzo kumasulidwa reagent, ndi m'zigawo wotsatira ayenera kuchitidwa molingana ndi malangizo ntchito Macro & yaying'ono-Test Sample Release. Reagent (HWTS-3005-8).
B: Tengani1.4mililita ya mkodzo chitsanzo kuyesedwa ndi centrifuge pa 12,000rpm kwa mphindi 5;kutaya supernatant (ndiko bwino kusunga 10-20μL wa supernatant kuchokera pansi pa chubu cha centrifuge), ndi kuwonjezera 200μL ya saline wamba kuti ayimitsenso, monga chitsanzo kuti ayesedwe.Kutulutsa kotsatira kumatha kuchitidwa ndi Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. motsatira malangizos za ntchito.Voliyumu yovomerezeka ndi 80μL.
C: Tengani1.4mililita ya mkodzo chitsanzo kuyesedwa ndi centrifuge pa 12,000rpm kwa mphindi 5;kutaya supernatant (ndiko bwino kusunga 10-20μL wa supernatant kuchokera pansi pa chubu cha centrifuge), ndi kuwonjezera 200μL ya saline wamba kuti ayimitsenso, monga chitsanzo kuti ayesedwe.Chotsatira chotsatira chikhoza kuchitidwa ndiQIAamp DNA Mini Kit(51304) yolembedwa ndi QIAGEN kapena Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).M'zigawozi ayenera kukonzedwa molingana ndi malangizo ntchito.Voliyumu yoyeserera ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
2. Mphepete mwa khomo lachiberekero
A: Tengani 1mL ya chitsanzo kuti muyesedwe mu 1.5mLof chubu cha centrifuge,ndicentrifuge pa 12000rpm kwa mphindi 5. Discard the supernatant (tikulimbikitsidwa kusunga 10-20μL ya supernatant kuchokera pansi pa chubu cha centrifuge), onjezerani 100μL ya reagent yotulutsa zitsanzo, kenako ndikuchotsani molingana ndi malangizo ogwiritsira ntchito Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Kutulutsa kumatha kuchitidwa ndi Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. motsatira malangizo ogwiritsira ntchito.Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
C: The m'zigawo akhoza kuchitidwa ndi QIAamp DNA Mini Kit (51304) ndi QIAGEN kapena Macro & yaying'ono-Test Viral DNA/RNA Column (HWTS-3020-50).M'zigawozi ayenera kukonzedwa molingana ndi malangizo ntchito.Voliyumu yachitsanzo yochotsa ndi 200 μL, ndipo voliyumu yovomerezeka ya elution ndi80 ml.
3, Khomo la Khomo/Kumaliseche Kwamaliseche
Musanayambe sampuli, ntchito thonje swab mofatsa misozi owonjezera secretions pa khomo pachibelekeropo, ndi ntchito thonje swab analowerera ndi njira kuteteza selo kapena khomo lachiberekero exfoliated selo sampling burashi kukakamira khomo lachiberekero mucosa ndi kutembenukira mozungulira 3-5 mozungulira kupeza. khomo lachiberekero exfoliated maselo.Pang'onopang'ono chotsani thonje swab kapena burashi,ndiIkani mu chubu lachitsanzo ndi 1mL ya saline wosabala wamba. AMukatsuka bwino, finyani zowumitsa thonje kapena burashi pakhoma la chubu ndikutaya, mangani kapu ya chubu, ndikulemba dzina lachitsanzo (kapena nambala) ndikulemba pa chubu chachitsanzo.
