Mitundu 14 ya Tizilombo Zoyambitsa Kupuma Zophatikizidwa
Dzina la malonda
HWTS-RT159B Mitundu 14 ya Tizilombo Zopuma Zophatikiza Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda opumira ndi matenda omwe amapezeka kwambiri mwa anthu, omwe amatha kuchitika mumtundu uliwonse, zaka komanso dera. Ichi ndi chimodzi mwazinthu zomwe zimayambitsa matenda komanso kufa kwa anthu padziko lonse lapansi[1]. Tizilombo toyambitsa matenda toyambitsa matenda timaphatikizira kachilombo ka coronavirus, kachilombo ka fuluwenza A, kachilombo ka fuluwenza B, kupuma kwa syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, St.[2,3].
Channel
| Chabwino Position | Rection Solution Dzina | Tizilombo toyambitsa matenda topezeka |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, control mkati |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, control mkati |
Technical Parameters
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Oropharyngeal swab, nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 Makopi / ml |
| Mwatsatanetsatane | Zotsatira za mayeso a cross-reactivity zidawonetsa kuti panalibe kulumikizana pakati pa zida izi ndi Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella-zoster virus, Legionellauttenal phracistral ph. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stekhomal bacteria, Cococcus, striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlaciimic nucleic acid pneumoniae, Chlatimic nucleic acid ndi Colamydia pneuella. |
| Zida Zogwiritsira Ntchito | SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Regent yovomerezeka yochotsa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) yolembedwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Masitepe wotsatira ayenera kuchitidwa molingana ndi malangizo ntchito m'zigawo reagent. The analimbikitsa elution voliyumu ndi80µl pa.
