Mitundu 14 ya Majeremusi Opumira Ophatikizidwa
Dzina la chinthu
HWTS-RT159B Mitundu 14 ya Majeremusi Opumira Combined Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda opatsirana m'mapapo ndi matenda ofala kwambiri mwa anthu, omwe amatha kuchitika mwa amuna kapena akazi, zaka, ndi dera lililonse. Ndi chimodzi mwa zifukwa zazikulu zomwe zimayambitsa matenda ndi imfa mwa anthu padziko lonse lapansi.[1]Matenda opatsirana omwe amapezeka m'mapapo ndi monga kachilombo ka corona, kachilombo ka Influenza A, kachilombo ka Influenza B, kachilombo ka Respiratory syncytial, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, ndi Streptococcus pneumoniae, ndi zina zotero.[2,3].
Njira
| Malo a Chitsime | Dzina la Yankho la Zokambirana | Tizilombo toyambitsa matenda tomwe tiyenera kuzindikirika |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, ulamuliro wamkati |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, ulamuliro wamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Chifuwa cha m'kamwa, chifuwa cha m'mphuno |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 2Makopi 00/mL |
| Kufotokozera Mwapadera | Zotsatira za mayeso obwerezabwereza zinasonyeza kuti panalibe kusinthana pakati pa zida izi ndi Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii ndi ma genomic nucleic acid a anthu. |
| Zida Zogwiritsidwa Ntchito | Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.)Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chotsukira chomwe chikufunidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchuluka kwa chitsanzo chochotsedwa ndi 200µL. Njira zotsatirazi ziyenera kuchitidwa motsatira malangizo ogwiritsira ntchito chotsukira ichi. Kuchuluka kwa chotsukira komwe kukufunidwa ndi80µL.
