Tizilombo Toyambitsa Kupuma Tophatikizana

Tizilombo Toyambitsa Kupuma Chithunzi Chophatikizidwa

Kufotokozera Kwachidule:

Bokosi ili limagwiritsidwa ntchito pozindikira matenda opatsirana m'mapapo mu nucleic acid yotengedwa kuchokera ku zitsanzo za swab ya oropharyngeal ya anthu.

Chitsanzochi chikugwiritsidwa ntchito pozindikira bwino za 2019-nCoV, kachilombo ka chimfine A, kachilombo ka chimfine B ndi ma nucleic acid a kachilombo ka respiratory syncytial mu zitsanzo za swab ya m'kamwa mwa munthu.

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Dzina la chinthu

HWTS-RT158A Combined Respiratory Pathogens Detection Kit (Fluorescence PCR)

Satifiketi

Epidemiology

Matenda a Corona Virus 2019, otchedwa'COVID 19', amatanthauza chibayo chomwe chimayambitsidwa ndi matenda a 2019-nCoV. 2019-nCoV ndi kachilombo ka corona komwe kali m'gulu la β. COVID-19 ndi matenda opatsirana opatsirana omwe amafalikira mofulumira, ndipo anthu ambiri amakhala pachiwopsezo. Pakadali pano, gwero la matendawa ndi odwala omwe ali ndi kachilombo ka 2019-nCoV, ndipo anthu omwe alibe zizindikiro za matendawa nawonso akhoza kukhala gwero la matendawa. Kutengera kafukufuku wamakono wa matenda, nthawi yoberekera ndi masiku 1-14, makamaka masiku 3-7. Malungo, chifuwa chouma ndi kutopa ndiye zizindikiro zazikulu. Odwala ochepa anali ndi zizindikiro monga kutsekeka kwa mphuno, mphuno yotuluka madzi, pakhosi, myalgia ndi kutsegula m'mimba, ndi zina zotero.

Fuluwenza, yomwe imadziwika kuti "fuluwenza", ndi matenda opatsirana opatsirana omwe amayamba chifukwa cha kachilombo ka fuluwenza. Ndi matenda opatsirana kwambiri. Amafalikira makamaka pokodza ndi kuyetsemula. Nthawi zambiri amafalikira m'nyengo ya masika ndi yozizira. Mavairasi a fuluwenza amagawidwa m'magulu atatu: fuluwenza A (IFV A), fuluwenza B (IFV B), ndi fuluwenza C (IFV C), onse ndi a kachilombo komatira, komwe kumayambitsa matenda a anthu makamaka a fuluwenza A ndi B, ndi kachilombo ka RNA kogawanika kamodzi. Kachilombo ka fuluwenza A ndi matenda opatsirana kupuma, kuphatikizapo H1N1, H3N2 ndi mitundu ina, yomwe imakonda kusintha ndi kufalikira padziko lonse lapansi. "Shift" imatanthauza kusintha kwa kachilombo ka fuluwenza A, komwe kumabweretsa "subtype" yatsopano ya kachilombo. Mavairasi a fuluwenza B amagawidwa m'mizere iwiri, Yamagata ndi Victoria. Kachilombo ka fuluwenza B kamangokhala ndi antigen drift, ndipo kamapewa kuyang'aniridwa ndi kuchotsedwa kwa chitetezo cha mthupi la munthu kudzera mu kusintha kwake. Komabe, liwiro la kusintha kwa kachilombo ka fuluwenza B limachepa kuposa la kachilombo ka fuluwenza A. Kachilombo ka fuluwenza B kangayambitsenso matenda opatsirana m'mapapo mwa anthu ndipo kamayambitsa miliri.

Kachilombo ka Respiratory Syncytial Virus (RSV) ndi kachilombo ka RNA, komwe kali m'gulu la paramyxoviridae. Kachilomboka kamafalikira kudzera m'madontho a mpweya komanso pafupi ndipo ndiye kachilombo kamene kamayambitsa matenda m'mapapo mwa makanda. Makanda omwe ali ndi kachilombo ka RSV amatha kukhala ndi matenda oopsa a bronchiolitis ndi chibayo, zomwe zimagwirizana ndi mphumu mwa ana. Makanda amakhala ndi zizindikiro zoopsa, kuphatikizapo malungo, rhinitis, pharyngitis ndi laryngitis, kenako bronchiolitis ndi chibayo. Ana ochepa odwala amatha kukhala ovuta ndi otitis media, pleurisy ndi myocarditis, ndi zina zotero. Matenda a m'mapapo ndi chizindikiro chachikulu cha matenda mwa akuluakulu ndi ana okulirapo.

Njira

FAM	SARS-CoV-2
VIC(HEX)	RSV
CY5	IFV A
ROX	IFV B
Quasar 705	Kulamulira Kwamkati

Magawo aukadaulo

Malo Osungirako	-18℃
Nthawi yokhalitsa	Miyezi 12
Mtundu wa Chitsanzo	Chidutswa cha m'kamwa
Ct	≤38
LoD	2019-nCoV: Makopi 300/mL Kachilombo ka Influenza A/kachilombo ka Influenza B/kachilombo ka kupuma kogwirizana ndi mpweya: 500Kopi/mL
Kufotokozera Mwapadera	a) Zotsatira za cross-reactivity zikuwonetsa kuti palibe cross reaction pakati pa kit ndi human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci and newborn cryptococcus and human genomic nucleic acid. b) Mphamvu yoletsa kusokoneza: select mucin (60mg/mL), 10% (v/v) ya magazi ndi phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (kuphatikiza zosungira) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ndi tobramycin (0.6mg/mL) kuti muyesedwe kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zosokoneza zomwe zatchulidwa pamwambapa sizisokoneza zotsatira za mayeso a tizilombo toyambitsa matenda.
Zida Zogwiritsidwa Ntchito	Dongosolo la PCR la BioRad CFX96 Real-Time Dongosolo la PCR la Rotor-Gene Q 5plex HRM Platform nthawi yeniyeni