Mtundu wa kachilombo ka polio Ⅱ
Dzina la chinthu
HWTS-EV007- Mtundu wa Poliovirus Ⅱ Kiti Yodziwira Nucleic Acid (Fluorescence PCR)
Epidemiology
Kachilombo ka polio ndi kachilombo komwe kamayambitsa poliomyelitis, matenda opatsirana omwe amafalikira kwambiri. Kachilombo kameneka nthawi zambiri kamalowa m'thupi la mitsempha, kumawononga maselo amitsempha omwe ali m'nyanga yakutsogolo ya msana, komanso kumayambitsa ziwalo zofooka, zomwe zimapezeka kwambiri mwa ana, motero zimatchedwanso polio. Kachilombo ka polio ndi ka mtundu wa enterovirus wa m'banja la picornaviridae.
Njira
| FAM | Mtundu wa kachilombo ka polio Ⅱ |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Chitsanzo cha ndowe zomwe zasonkhanitsidwa kumene |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 1000/mL |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time, Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Ma reagents ofunikira ochotsera: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Kuchotsa kuyenera kuchitika motsatira IFU mosamalitsa. Kuchuluka kovomerezeka kwa elution ndi 80μL.
Njira yachiwiri.
Ma reagents ofunikira ochotsera: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Kuchotsa kuyenera kuchitika motsatira IFU mosamalitsa. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 100μL.
